Cell culture medium beneficial to in-vitro culture of autologous bone marrow mesenchymal stem cells

A bone marrow mesenchymal, in vitro culture technology, applied in the direction of bone/connective tissue cells, animal cells, vertebrate cells, etc., can solve problems such as unfavorable therapeutic applications, avoid exogenous infectious diseases, simple preparation process, guarantee The effect of proliferative capacity

Inactive Publication Date: 2015-03-25
黄相杰
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, the cell culture medium used in the process of culturing and expanding bone marrow mesenchymal stem cells generally needs to add fetal calf serum, and some also add some other cell growth factors to maintain the culture and expansion of cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell culture medium beneficial to in-vitro culture of autologous bone marrow mesenchymal stem cells
  • Cell culture medium beneficial to in-vitro culture of autologous bone marrow mesenchymal stem cells
  • Cell culture medium beneficial to in-vitro culture of autologous bone marrow mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A cell culture medium conducive to the in vitro culture and expansion of autologous bone marrow mesenchymal stem cells:

[0025] The complete medium used for primary cell culture has a final concentration as follows: PL platelet lysate 5-20%, heparin 2-5U / ml, doxycycline 5-10 μg / ml, solvent as basal medium, The basic medium is DMEM medium;

[0026] The complete medium used for cell culture in the first generation and after the first generation has a final concentration as follows: PL platelet lysate 5-20%, heparin 2-5U / ml, doxycycline 5-10 μg / ml, The solvent is the basal medium, and the basal medium is AMEM culture fluid.

[0027] The percentage of PL in the complete medium of cells is added according to the age of the person, the percentage of PL added is 5-10% for those less than or equal to 45 years old, and the percentage of PL added for those older than 45 years is 10-20%.

[0028] A method for preparing a cell culture medium conducive to in vitro culture and exp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of cell culture media, and discloses a cell culture medium beneficial to in-vitro culture of autologous bone marrow mesenchymal stem cells. The cell culture medium is characterized in that an autologous PL blood platelet lysis buffer is added during preparation. The autologous bone marrow mesenchymal stem cells are cultured by using the cell culture medium added with the PL blood platelet lysis buffer so as to ensure that the cell proliferation capability of cells in in-vitro culture; and meanwhile, a phenomenon that the mesenchymal stem cells cannot be affected by exogenous pollution in in-vitro culture and amplification can be ensured, the immunological rejection of allogeneic cells generated in a disease treatment process can also be avoided, and an application of treating self-diseases by using autologous cells clinically can be facilitated.

Description

technical field [0001] The invention relates to the technical field of cell culture medium, in particular to a kind of cell culture medium which is beneficial to the in vitro culture and expansion of autologous bone marrow mesenchymal stem cells. Add the cell culture medium of autologous PL platelet lysate to the cell culture medium. Background technique [0002] In recent years, the cell culture medium used in the process of culturing and expanding bone marrow mesenchymal stem cells generally needs to add fetal calf serum, and some also add some other cell growth factors to maintain the culture and expansion of cells. When fetal bovine serum is added to the cell culture medium, the fetal bovine serum will contain foreign proteins, and may carry bacteria, viruses, protein infectious diseases or prions into the cells, which will not be conducive to clinical treatment application. The present invention uses autologous PL platelet lysate to replace fetal bovine serum and some...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/0775
Inventor 黄诚黄相杰姜红江王玉鹤
Owner 黄相杰
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap