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A kind of exopolysaccharide of Lactobacillus rhamnosus and its preparation method and application

A technology of Lactobacillus rhamnosus and exopolysaccharides, applied in the direction of microorganism-based methods, biochemical equipment and methods, food science, etc., can solve the problems of insufficient variety of lactic acid bacteria exopolysaccharides and product functions, and achieve remarkable technology Advantages, broad application prospects, and clear application range

Active Publication Date: 2017-10-27
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a new exopolysaccharide of lactic acid bacteria, specifically a kind of exopolysaccharide of Lactobacillus rhamnosus, aiming at the current situation that the types of exopolysaccharides of lactic acid bacteria and product functions are not rich enough, the present invention Also provide the preparation method and application of the exopolysaccharide

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  • A kind of exopolysaccharide of Lactobacillus rhamnosus and its preparation method and application
  • A kind of exopolysaccharide of Lactobacillus rhamnosus and its preparation method and application
  • A kind of exopolysaccharide of Lactobacillus rhamnosus and its preparation method and application

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Embodiment 1

[0053] The preparation of embodiment 1 exopolysaccharide

[0054] Pick freshly cultured Lactobacillus rhamnosus CGMCCNo.6430 colony from the MRS agar plate of 37 ℃, anaerobic culture for 48h, and transfer to 12% (w / w) no Bacteria skimmed milk medium, cultured at 32°C for 20h, to obtain seed liquid. Then, the inoculum was transferred to the above-mentioned aseptic skim milk medium according to the volume ratio of 1%, and fermented at 36° C. for 40 hours to obtain fermented milk of Lactobacillus rhamnosus CGMCC NO.6430. Heat the fermented milk at 95°C for 30min, and after cooling to room temperature, centrifuge (4°C, 14,000g, 20min) to remove bacteria and coagulated protein; add 85% (w / w) trichloroacetic acid to the supernatant until final Concentration of 8.0% (w / v) was allowed to stand overnight, centrifuged (4°C, 14,000g, 20min) to remove precipitated protein; the supernatant was added to 4 times the volume of 80% ethanol, allowed to stand overnight at 4°C, centrifuged (4°C,...

Embodiment 2

[0056] The preparation of embodiment 2 extracellular polysaccharides

[0057] Pick freshly cultivated Lactobacillus rhamnosus CGMCCNo.6430 colony from the MRS agar plate cultured at 37°C and anaerobic for 48h, transfer it to sterile skim milk medium, cultivate at 32°C for 20h, and obtain seed liquid. The aseptic skim milk culture medium is the aseptic skim milk culture medium obtained by dissolving 10 parts by weight of skim milk and 0.8 parts by weight of glucose in 8 parts by weight of water, sterilizing at 115° C. for 20 minutes, and cooling to 25° C. Then, the inoculation amount of 3% by volume was transferred to the above-mentioned aseptic skim milk medium, and fermented at 32° C. for 30 h to obtain fermented milk of Lactobacillus rhamnosus CGMCC NO.6430. Heat fermented milk at 100°C for 10min, and after cooling to room temperature, centrifuge (4°C, 14,000g, 20min) to remove bacteria and coagulated protein; add 80% (w / w) trichloroacetic acid to the supernatant until final...

Embodiment 3

[0059] The preparation of embodiment 3 extracellular polysaccharides

[0060] Pick freshly cultivated Lactobacillus rhamnosus CGMCCNo.6430 colony from the MRS agar plate cultured at 37°C and anaerobic for 48h, transfer it to sterile skim milk medium, cultivate at 32°C for 20h, and obtain seed liquid. The aseptic skim milk culture medium is the aseptic skim milk culture medium obtained by dissolving 12 parts by weight of skim milk and 1.2 parts by weight of glucose in 90 parts by weight of water, sterilizing at 125° C. for 15 minutes, and cooling to 15° C. Then, the inoculation amount of 5% by volume was transferred to the above-mentioned aseptic skim milk medium, and fermented at 28° C. for 24 hours to obtain fermented milk of Lactobacillus rhamnosus CGMCC NO.6430. Heat the fermented milk at 98°C for 20min, and after cooling to room temperature, centrifuge (4°C, 14,000g, 20min) to remove bacteria and coagulated protein; add 75% (w / w) trichloroacetic acid to the supernatant unt...

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Abstract

The invention discloses an exopolysaccharide of Lactobacillus rhamnosus, a preparation method and application thereof. The exopolysaccharide is composed of 45.13-47.80% glucose, 35.26-36.17% rhamnose and 16.94-18.70% galactose in a mass fraction ratio. The exopolysaccharide is composed of repeating structural units shown in formula I. The average weight and molecular weight of exopolysaccharide is 980,000-1.47 million Daltons. It is a new type of polysaccharide with a clear composition and certain immune regulation effect. It can be used as an additive in pharmaceutical and clinical fields, and its application prospect is very broad.

Description

[0001] This application claims the priority of the Chinese patent application CN201310743058.0 with the filing date of December 27, 2013. This application cites the full text of the Chinese patent application. technical field [0002] The invention relates to an exopolysaccharide of Lactobacillus rhamnosus, a preparation method and application thereof. Background technique [0003] Lactic acid bacteria is a general term for bacteria that can use fermentable sugars to produce a large amount of lactic acid. There are at least 23 taxonomic genera of such bacteria that have been found in nature. The lactic acid bacteria that are widely used in the fields of food and medicine mainly include Lactobacillus, Streptococcus, Enterococcus, Lactococcus, Pediococcus and Leuconostoc. Lactic acid bacteria are the main source of probiotics. Many lactic acid bacteria are inherent probiotics in the human intestinal tract. They have important physiological activities such as improving the hum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12P19/04A23L33/125A61P35/00A61P37/02C12R1/225A23L29/30
Inventor 吴正钧郭本恒韩瑨邵丽杭锋刘振民
Owner BRIGHT DAIRY & FOOD CO LTD
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