Visualization calibration laser-induced fluorescence detection device

Abstract

The invention discloses a visualization calibration laser-induced fluorescence detection device. The visualization calibration laser-induced fluorescence detection device comprises a dichroscope which is obliquely arranged in a dichroscope frame, a laser incidence device which is arranged in a laser incidence direction of the dichroscope, a lens which is arranged in a laser refraction direction of the dichroscope and a transmitting filter plate which is arranged in a fluorescence receiving direction of the dichroscope, wherein a capillary tube with a window is arranged on a focal point at the outer side of the lens, an adjustable slit and a photomultiplier tube are sequentially arranged at the outer side of the transmitting filter plate, the dichroscope frame is provided with a cross division plate in the laser transmission direction of the dichroscope, and an endoscope is arranged at the outer side of the cross division plate. The visualization calibration laser-induced fluorescence detection device has advantages that the laser incidence light is collimated, the laser incidence light position is displayed online in real time, and the visualization calibration can be realized. The replacement of a variable-wavelength laser-induced fluorescence detection device can be realized, the stability of an output light path system when different lasers with different wavelengths are used can be guaranteed, and the applicability and stability of the detector can be improved.

Description

Technical field [0001] The invention belongs to a detection and analysis instrument, and particularly relates to a laser-induced fluorescence detection device that is mainly suitable for the detection of trace samples in the fields of drug clinical and biological sciences. Background technique [0002] In the 1970s, Zare et al. first proposed the laser-induced fluorescence detection technology (LIF). Due to its high detection sensitivity and the characteristics of being suitable for micro-area detection, the LIF detection system has achieved rapid development and wide application. With the rapid development of micro-separation technology in recent years, scientific and technological workers have higher requirements for the separation efficiency, analysis speed, and detection sensitivity of existing analysis methods. Especially for the demand for detection technology, they tend to be more sensitive. , Highly selective direction. Laser-induced fluorescence detection technology (LI...

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Problems solved by technology

However, the laser, dichroic mirror and filter set will cause the shift of the optical path of the entire system during the replacement process, and there is no reference standard for optical path calibration in the adjustment of the optical path

The previous method was to observe the brightness of the fluorescence excited by the fluorescent substance in the detection window. At this time, the laser light directly irradiates the capillary window to generate a large amount of scattered light. It is difficult to distinguish the position with the highest brightness, and it is easy to damage the eyes, and the operation is troublesome; Calibrate the optical path according to the magnitude of the output electrical signal of the photomultiplier tube. At this time, the laser position can be confirmed according to the highest point of the Gaussian curve of the electrical signal, but the position where the laser irradiates on the capillary cannot be observed. If the optical path offset is large, The operating time is also extended accordingly

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