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Quality control method of sweet tea

A technology of sweet tea and its detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low yield, low purity of sweet tea, high production cost, etc., achieve fast separation speed, simple and easy method, and short production cycle short effect

Inactive Publication Date: 2017-01-18
广西新桂环保科技集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The object of the present invention is to provide a preparation and quality control method of high-purity sweet tea in order to overcome the existing shortcomings of low purity, low yield or high production cost of sweet tea, as well as the non-systematic sweet tea extraction and purification and quality control methods , the sweet tea prepared by this method has high purity and good quality, and can be used as a chemical reference substance or standard substance, and the quality control method is effective, stable and uniform

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  • Quality control method of sweet tea

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1: the preparation of high-purity sweet tea

[0044] Take the dried leaves of sweet tea, add water to decoct and extract 3 times, the amount of water added each time is 8 times the weight of the medicinal materials, and the extraction time is 2 hours each time, the extracts are combined, filtered, and the filtrate is concentrated to obtain an extract, which is absorbed by a macroporous resin column Use ethanol-water (0:100~60:40) gradient elution, collect ethanol-water (60:40) eluted part, concentrate, and obtain crude rubabu crystals.

[0045] The crude crystals are prepared by preparative RP-HPLC (chromatographic column: C-18 column; mobile phase: methanol: water volume ratio is 65:35; detection wavelength is 205nm; flow velocity is 5ml / min), and the collected purity is 90 The eluate between %-98% and purity greater than 98% is concentrated under reduced pressure to obtain sweet tea with a purity between 90%-98% and greater than 98%.

[0046] All preparati...

Embodiment 2

[0049] Embodiment 2: the preparation of high-purity sweet tea

[0050] Take the dried leaves of sweet tea, add water to decoct and extract 2 times, each time add 10 times the weight of the medicinal materials, each time the extraction time is 3 hours, filter, combine the filtrate, concentrate, get the extract, the extract is absorbed by the macroporous resin column , using ethanol-water (0:100-70:30) gradient elution, collecting ethanol-water (40:70) eluted fractions, concentrating, and obtaining crude sweet tea crystals.

[0051] The crude crystals are prepared by preparative RP-HPLC (chromatographic column: C-18 column; mobile phase methanol: water volume ratio is 60:40; detection wavelength is 205nm; flow rate is 5ml / min), and the collected purity is 90% The eluate between -98% and purity greater than 98% is concentrated under reduced pressure to obtain sweet tea with a purity between 90% -98% and greater than 98%.

[0052] All preparation liquids are detected by HPLC anal...

Embodiment 3

[0053] Embodiment 3: the preparation of high-purity sweet tea

[0054] Take the dried leaves of sweet tea, add water to decoct and extract 8 times, the amount of water added each time is twice the weight of the medicinal materials, and the extraction time is 1 hour each time, filter, combine the filtrates, concentrate, and obtain extracts, and the extracts are absorbed by macroporous resin columns , using ethanol-water (0:100-50:50) gradient elution, collecting ethanol-water (80:20) eluted fractions, concentrating, and obtaining rough crystals of sweet tea.

[0055] Detect with HPLC, collect the fractions containing sweet tea, combine, concentrate, and obtain sweet tea crude crystals. The crude crystals are prepared by preparative RP-HPLC (chromatographic column: C-18 column; mobile phase methanol: water volume ratio is 50:50; detection wavelength is 205nm; flow rate is 5ml / min), and the collected purity is 90% The eluate between -98% and purity greater than 98% is concentrat...

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Abstract

The invention discloses a preparation method and a quality control method of high-purity rubusoside by use of rubusoside rosaceae rubus plants as raw materials. The method is characterized in that the method comprises the steps of extracting rubusoside plants, adsorbing with a macroporous resin column, detecting through the HPLC (High Performance Liquid Chromatography), collecting the flow ingredient containing rubusoside, separating by a reverse-phase preparative high-performance liquid phase method, detecting each part of the collected eluant by use of analytical type HPLC, mixing and keeping the high-purity rubusoside component, and carrying out quality control by the thin-layer chromatography and the liquid chromatogram. According to the method, the purity of the rubusoside can be improved and the production cost can be reduced.

Description

technical field [0001] The invention relates to the field of a method for quality control of medicinal materials, in particular to a method for quality control of sweet tea. Background technique [0002] Rubeta is a diterpene glycoside chemical component, one of the active components of plants, and an index component for standard quality control of many plants and medicines. At present, there is no corresponding national drug standard substance, and there is no report on the systematic research on the reference substance of sweet tea at home and abroad. With reference to the technical requirements of the chemical reference substance of traditional Chinese medicine (for content determination), the chemical reference substance of sweet tea is studied, and the establishment of sweet tea Batch extraction process, purity and content, and analysis and determination methods for chemical reference substances, as well as impurity inspection, so as to establish technical standards for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/36G01N30/88
Inventor 黄艳刘布鸣莫建光冯军柴玲
Owner 广西新桂环保科技集团有限公司
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