Group A rotavirus chromatography test paper strip based on low-noise excitation fluorescent label

A group A rotavirus, fluorescent labeling technology, applied in analytical materials, biological tests, material inspection products, etc., can solve the problem of difficult and rapid quantitative detection of rotavirus, and achieve high signal-to-noise ratio, improved sensitivity, and high detection. The effect of sensitivity

Inactive Publication Date: 2015-04-08
BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] For this reason, the technical problem to be solved by the present invention lies in the problem that it is difficult to rapidly quantitatively detect rotavirus in the prior art, and then provide a kind of A group rotavirus immunochromatographic test strip based on low-noise excitation fluorescent labeling, and It further discloses its application

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  • Group A rotavirus chromatography test paper strip based on low-noise excitation fluorescent label
  • Group A rotavirus chromatography test paper strip based on low-noise excitation fluorescent label
  • Group A rotavirus chromatography test paper strip based on low-noise excitation fluorescent label

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Embodiment 1

[0041] The group A rotavirus immunochromatographic test strips based on low-noise excitation fluorescent labeling described in this embodiment are as follows: figure 1 As shown, it includes a bottom plate 7 and a sample pad 1, a binding pad 2, an antibody-carrying membrane 3 and a water-absorbent pad 6 that are sequentially adhered to the bottom plate along the length direction of the bottom plate 7, and the sample pad 1 , binding pad 2, antibody-carrying membrane 3 and water-absorbing pad 6, which are sequentially in contact with and only partially overlap adjacent parts; the antibody-carrying membrane 3 is adhered to the middle part of the bottom plate 7, and A quality control line 5 with spaced detection lines 4 is provided, the detection line 4 is close to the binding pad 2 , and the quality control line 5 is close to the water-absorbing pad 6 . The quality control line 4 is arranged parallel to the detection line 5, and the distance between the detection line and the qual...

Embodiment 2

[0056] This embodiment provides a method for qualitatively detecting rotavirus by using the above-mentioned group A rotavirus immunochromatography test strip based on low-noise excitation fluorescent labeling, including the following steps:

[0057] a) Take 25 g of strawberry samples, dilute the samples with 225 mL of PBS buffer solution containing 5% BSA and 0.1% Tween 20, take the supernatant as a test sample, and draw 1 ml of the supernatant for use. Draw 50 μL of the pretreated test sample and drop it on the sample pad 1, after the absorption is dry, add 50 μL of the chromatographic solution dropwise, and take the buffer as a negative control, and use the test strip to perform immunochromatography;

[0058] b) After standing at room temperature for 15 minutes, use a portable low-noise excitation fluorescence scanner (Beijing Borun Fude Technology Development Co., Ltd.) to measure the fluorescence intensity of the detection line and the quality control line area respectively...

Embodiment 3

[0061] This embodiment provides a method for quantitatively detecting rotavirus by using the rotavirus immunochromatography test strip based on low-noise excitation fluorescent labeling.

[0062] (a) Preparation of standard curve: take 100 μg / ml of Group A rotavirus capsid protein (Beijing Huada Protein Company), and dilute the described solution with 5% BSA, 0.1% Tween 20, and PBS (pH7.2) diluent The protein was serially diluted to make 0.5, 1, 2.5, 5, 10, 20 μg / ml samples.

[0063] (b) Take more than 50 μL of sample and drop it on the sample pad 1. After the sample is absorbed, add 50 μL of chromatographic solution dropwise, let it stand at room temperature for 10 minutes, and put the above test strip into a portable low-noise excitation fluorescence scanner (Beijing Readings in Borun Fude Technology Development Co., Ltd.). The above-mentioned serially diluted rotavirus standard products of group A were detected respectively, and the fluorescence values ​​of the detection l...

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Abstract

The invention belongs to the field of food detection, and particularly relates to a group A rotavirus chromatography test paper strip based on low-noise excitation fluorescent label as well as a preparation method and application of the chromatography test paper strip. According to the invention, quantum dots are adopted as the label, the immunochromatography technology is adopted, so that the chromatography test paper strip targeting to group A rotavirus is prepared, during detection, a special portable low-noise excitation fluorescent scanner is adopted for scanning a quality control line and a detection line, and qualitative and quantitative determination of a sample is realized through a fluorescence intensity detection value of the detection line. The test paper strip is used for immediate detection of group A rotavirus in food and a pathological sample, and has the advantages of rapidness, high sensitivity, qualitative, accurate and quantitative detection, and portability.

Description

technical field [0001] The invention belongs to the field of food (medicine) detection, and in particular relates to a group A rotavirus immunochromatographic test strip based on low-noise excitation type fluorescent dye labeling and its preparation method and application. Background technique [0002] Rotavirus is one of the main pathogens that cause diarrhea in infants and young children. It mainly infects small intestinal epithelial cells, causing cell damage and diarrhea. Rotavirus is prevalent in summer, autumn and winter every year. The route of infection is the fecal-oral route. The clinical manifestations are acute gastroenteritis and osmotic diarrhea. It exists widely in nature. The rotavirus in food is dangerous to human safety. The bacterium can still grow and reproduce at low temperature. It is one of the main pathogenic bacteria that threaten human health in refrigerated food. Therefore, in the microbiological inspection of food hygiene , must be paid attention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/533
CPCG01N33/68G01N33/533
Inventor 张捷张岩杨向莹李锦丰张惠媛刘洁张晓光舒咬根陈广全
Owner BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT
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