MACA extract, preparation method and application thereof

A maca extract and sub-extraction technology, which is applied in the direction of drug combination, disease, organic chemistry, etc., can solve the problems of low content of active ingredients, complex extraction and purification process of macamide, and low use potency, so as to achieve easy industrialization The effect of strong adaptability of production and production equipment and simple process

Active Publication Date: 2015-04-15
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The utilization of maca in the prior art mostly adopts maca raw powder or simple crude extract, and there is little research on the purification of active ingredients in maca, even existing maca raw powder or crude extract also exists Low content of active ingredients, low use potency and other disadvantages
Existing extraction and purification process of macamide is complicated, it is difficult to carry out a large amount of industrial production, etc.

Method used

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  • MACA extract, preparation method and application thereof
  • MACA extract, preparation method and application thereof
  • MACA extract, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A preparation method of maca extract, comprising the steps of:

[0052] (1) Weigh 200g of dried maca roots and crush them, pass through a 40-mesh sieve, add 1000mL of 20% ethanol, stir and extract at 20°C for 24 hours, and centrifuge at 5000rpm for 10 minutes; after the residue is extracted and centrifuged again, combine the two extraction supernatants; above The clear liquid was concentrated under reduced pressure to a viscous shape to obtain a crude Maca extract;

[0053] (2) Add 100mL of water to dissolve, then add an equal volume of ethyl acetate, shake vigorously for 5 minutes and let it stand for 30 minutes, then take the ethyl acetate layer; repeat the extraction of the water layer 3 times; combine the ethyl acetate layers, concentrate and dry under reduced pressure to obtain coffee primary extract as raw material for purification;

[0054] (3) Purify the maca primary extract:

[0055] ①Dissolve the extract in 50mL of methanol and put it on a reversed-phase C18 c...

Embodiment 2

[0058] A preparation method of maca extract, comprising the steps of:

[0059] (1) Weigh 10g of dried maca roots and crush them, pass through a 40-mesh sieve, add 1000mL of pure ethanol, extract with 800W ultrasonic at 50°C for 30min, and centrifuge at 5000rpm for 10min; Extract;

[0060] (2) Add 20mL of water to dissolve, then add an equal volume of petroleum ether, shake vigorously for 5 minutes, and then stand still for 30 minutes to take the petroleum ether layer; repeat the extraction of the water layer for 3 times, combine the petroleum ether layer, concentrate and dry under reduced pressure to obtain the primary extract of maca , as a raw material for purification;

[0061] (3) Purify the maca primary extract:

[0062] ①Use 20 mL of methanol to dissolve the extract and put it on a reversed-phase C18 column, and perform 3-stage isocratic washing with three concentrations of methanol aqueous solution with 6 column volumes of 30%, 6 column volumes of 50% and 1 column vol...

Embodiment 3

[0065] A preparation method of maca extract, comprising the steps of:

[0066] (1) Weigh 10g of dried maca root, crush it, pass through a 20-mesh sieve, add 50mL of 55% ethanol, extract with 800W ultrasonic wave at 20°C for 30min, and centrifuge at 5000rpm for 10min; the supernatant is concentrated under reduced pressure until viscous to obtain maca crude extract;

[0067] (2) Add 20 mL of water to dissolve, then add an equal volume of petroleum ether, shake vigorously for 5 minutes, and then stand still for 30 minutes to take the petroleum ether layer; repeat the extraction of the water layer 3 times, combine the petroleum ether layer, concentrate and dry under reduced pressure to obtain maca once extracted as raw materials for purification;

[0068] (3) Purify the maca primary extract:

[0069] ①Dissolve the extract in 20mL of methanol and put it on a reversed-phase C18 column, and perform 3-stage isocratic washing with three concentrations of methanol aqueous solution wit...

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Abstract

The invention relates to a preparation method of an MACA extract. The preparation method comprises following steps: (1) extracting MACA with a solvent to obtain a primary MACA extract as a purification raw material; and (2) continuously performing purification to obtain the MACA extract being high in the content of MACAmide, wherein the purification is carried out in a column chromatography manner. The column chromatographic purification comprises following steps: (1) performing impurity washing to remove impurities; and (2) performing elution to obtain the MACAmide. The MACA extract is high in the content of the MACAmide so that the MACA extract is excellent in effects of enhancing male fertility and treating male sexual dysfunction. The preparation method is simple in extraction technology, is easy to operate, has a strong adaptability in production equipment and is easy to achieve industrialized production.

Description

technical field [0001] The invention belongs to the field of biological preparation, and relates to a macamide extract, its preparation method and its application, and in particular to a maca amide extract with relatively high macamide content, its preparation method and its application. Background technique [0002] Maca (Lepidium meyenii Walp.) is an annual herbaceous plant belonging to the genus Brassicaceae. It is native to the Andes Mountains in South America at an altitude of 3500-4500m. my country began to introduce and plant maca in Lijiang, Yunnan in 2002, and it has become the world's largest maca planting base. Maca has rich nutritional value and medicinal value. Studies have shown that Maca has the functions of improving the fertility of humans and animals, improving sexual function, inhibiting prostate enlargement, anti-fatigue, anti-depression, improving osteoporosis, and improving memory. [0003] Macaamide is a unique secondary metabolite in Maca. It is a s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C231/24C07C233/05C07C233/09C07C235/76C07C233/20A61P15/08A61P15/00
Inventor 赵兵陈金金赵庆生董贝涛
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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