Hybridoma cell strain secreting monoclonal antibody against cucumber mosaic virus and application of monoclonal antibody
A technology of cucumber mosaic virus and hybridoma cell lines, applied in the direction of antiviral immunoglobulin, analytical materials, instruments, etc., can solve the problems of low efficiency and achieve the effect of sensitive detection
Active Publication Date: 2015-04-15
ZHEJIANG UNIV
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[0004] In order to grasp the incidence and hazards of cucumber mosaic virus (CMV) disease in my country, strengthen the early monitoring and early warning of cucumber mosaic virus disease in my country, and scientifically guide the prevention and control, it is urgent to establish a high-throughput detection method for the detection of cucumber mosaic virus disease. At present, there is no such high-throughput detection method, and only low-efficiency electron microscope observation, RT-PCR method, nucleic acid electrophoresis and other methods are used for small sample detection
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[0012] Hybridoma cell line 21H5 secreting anti-cucumber mosaic virus monoclonal antibody, which can secrete specific anti-cucumber mosaic virus monoclonal antibody, hybridoma cell line 21H5 was preserved in the Institute of Microbiology, Chinese Academy of Sciences on July 3, 2014 General Microbiology Center of China Microbiological Culture Collection Management Committee, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, postcode: 100101, and the preservation number is CGMCC No. 9342,
[0013] Anti-cucumber mosaic virus monoclonal antibody ascites indirect ELISA titer up to 10 -8 , the antibody type and subclass are IgG1, kappa chain, the monoclonal antibody can have specific immune binding reaction with the capsid protein of cucumber mosaic virus, and can detect the cucumber mosaic virus in the virus transmission medium aphid.
[0014] The monoclonal antibody against cucumber mosaic virus has specific immune reaction only with cucumber mosaic virus, ...
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Abstract
The invention discloses a hybridoma cell strain secreting monoclonal antibody against cucumber mosaic virus and application of the monoclonal antibody. Purified virus particles of cucumber mosaic virus (CMV) are used as an antigen to immunize BALB / c mice; through cell fusion, screening and cloning, one strain of hybridoma cell strain 21H5 capable of stable passage and secreting anti-CMV monoclonal antibodies is obtained; and the strain has a preservation number CGMCC No.9342. The monoclonal antibody secreted from the hybridoma cell strain has ascites indirect ELISA titer of 10<-8>, and the antibody type and subtype are IgG1, kappa chain. The monoclonal antibody has specificity reaction with CMV. The 21H5 antibody is used to establish a dot-ELISA virus method for detection of CMV in virus transmission medium aphid and in cucumber. The hybridoma cell strain 21H5, monoclonal antibody thereof and the establishment of the related serological detection method provide technical and material support for the diagnosis, detection, prediction and scientific prevention and control of the disease.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell strain secreting anti-cucumber mosaic virus monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Cucumber mosaic virus (CMV) is a single-stranded positive-sense RNA (+ssRNA) virus, a representative member of the Cucumber mosaic virus genus in the Bromovirus family. CMV can infect more than 1000 kinds of plants including monocotyledonous and dicotyledonous plants, and is one of the important and devastating pathogens of many crops and ornamental plants. CMV is mainly transmitted through host plant seeds or reproductive materials and insects in nature. It is currently known as the plant virus with the most hosts, the widest distribution and the most economic harm. Since CMV was first reported as the pathogen of cucumber mosaic disease in 1916, domestic and foreign scholars have successively reported the harm of the virus on...
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IPC IPC(8): C12N5/20C07K16/10G01N33/577G01N33/569C12R1/91
Inventor 周雪平吴建祥谢艳
Owner ZHEJIANG UNIV