Genetically engineered bacterium capable of expressing cell-penetrating peptide fusion protein and application

A technology for genetically engineering bacteria and proteins, applied in the field of genetic engineering, can solve the problems of asynchrony of virus speed, decrease of virus activity, increase of virus titer, etc., and achieve the effects of convenient large-scale production, low cost and simple operation process.
CN104530241AInactive Publication Date: 2015-04-22武汉市畜牧兽医科学研究所
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
武汉市畜牧兽医科学研究所
Publication Date
2015-04-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a genetically engineered bacterium capable of expressing cell-penetrating peptide fusion protein and application. The genetically engineered bacterium E.coli BL21 / pET-32a-CSFR with CCTCC NO: M2014631. The strain can be used for expressing protein transduction domain polypeptide (PEP-1) and hog cholera virus acceptor (CD46) genetically engineered fusion protein PEP-1-CD46. The genetically engineered bacterium has the advantages of large expression amount, soluble protein, low cost and high safety. The expressed PEP-1-CD46 protein can be used as mediated protein of hog cholera virus penetrating a cell membrane, so that the virus titer of hog cholera virus can be improved, and the defect that the virus content in a current hog cholera virus cell vaccine is low and cannot achieve the standard of vaccine preparation can be overcome.
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Description

Technical field

[0001] The present invention is a genetic engineering field. Specifically, a genetic engineering bacteria and applications expressing the messemid peptide fusion protein. technical background

[0002] Classical Swine Fever (CSF) is a kind of acute, fever, and mortality infectious disease caused by the Classical Swine Fever Virus (CSFV).The disease is highly contagious and has been widely popular internationally. The incidence rate is high and the mortality rate is as high as 90 %, which is extremely harmful.Entering important livestock and poultry infectious diseases.At present, my country is the country with the most production and use of swine fever vaccines in the world. The total consumption of about 4 billion yuan each year is about 4 billion yuan, and the total market price is about 2 billion yuan.

[0003] The long -term vaccine immunity and the interference of other epidemic diseases have gradually developed tolerance to swine fever vaccines. The response ...

Claims

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