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A Disperse Pantoea capable of producing bacteriocin and its screening method and application

A technology for dispersing Pantoea and bacteriocin, applied in the field of biotechnology and medicine, can solve the problem of HIV co-infection and TB without therapeutic effect, and achieve good clinical use value

Inactive Publication Date: 2017-10-13
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this standard therapy is ineffective against MDR-TB, XDR-TB, and HIV-coinfected TB
Moreover, almost no new anti-tuberculosis drugs have been on the market in the past 20 years. Therefore, new anti-tuberculosis drugs are urgently needed to control tuberculosis

Method used

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  • A Disperse Pantoea capable of producing bacteriocin and its screening method and application
  • A Disperse Pantoea capable of producing bacteriocin and its screening method and application
  • A Disperse Pantoea capable of producing bacteriocin and its screening method and application

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Embodiment 1

[0054] A disperse Pantoea strain capable of producing bacteriocins, the bacterium is WH-1, belongs to the genus Pantoea, and is preserved in the China Center for Type Culture Collection with the preservation number CCTCC No: M2014508.

[0055] The storage time of this bacterium was October 23, 2014; its taxonomic name is: Pantoea dispersa WH-1, Pantoea dispersa WH-1; the colonies of this bacterium are small and round, with thicker yellow protrusions and smooth edges. The cells are straight rod-shaped, 0.5-1.0μm×1-3μm, move with perinatal flagella, can produce yellow pigment, and are a kind of chemoheterotrophic bacteria with metabolic and fermentative types. It can use D-glucose and other sugars to produce acid, but not gas. Oxidase negative, catalase positive, indole negative, lysine and ornithine decarboxylase, and arginine dihydrolase negative. produce H 2 S, unhydrolyzed urea, malonate utilization is variable among strains, can reduce nitrate; fermentable sugars include:...

Embodiment 2

[0064] Please refer to figure 2 , the present embodiment provides a screening method for dispersing Pantoea, and the desired dispersing Pantoea (P. Double-layer agar plate for screening: first pour the bottom solid medium 7H10 into the petri dish, and then pour it into the semi-solid culture containing the indicator strain (Mycobacterium smegmatis) and the solution sample after solidification, specifically including the following steps:

[0065] Step 101: collect soil samples, add PBS buffer solution therein, oscillate and mix and filter to obtain a filtrate solution;

[0066] Specifically, in this step, in order to obtain a soil sample with a high content of microorganisms to be separated, preferably, the soil sample is taken from the moist soil around the organic fertilizer in the countryside, and the sampling depth is 5-10 cm. More specifically, the sample collection location is Yanling County, Henan Province, and the sampling time is October 2, 2011. The 5-point sampling...

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Abstract

The invention relates to the fields of biotechnology and medicine, in particular to a strain of Pantoea disperse capable of producing bacteriocins and its screening method and application. The disperse Pantoea strain capable of producing bacteriocin is characterized in that the bacterium is WH‑1, which belongs to the genus Pantoea, and is preserved in the China Center for Type Culture Collection with a preservation number of CCTCC No: M2014508. The dispersed Pantoea capable of producing bacteriocins provided by the present invention has the activity of inhibiting tuberculosis pathogenic bacteria (Mycobacterium tuberculosis and Mycobacterium bovis) through culture, and can be used as a drug adjuvant in combination with first-line anti-tuberculosis drugs Application, control treatment for tuberculosis and MDR‑TB, XDR‑TB, HIV-coinfected TB. In addition, experiments have proved that the bacteriocin produced by the bacteria can reduce the infection of Mycobacterium tuberculosis in mice within two days, so that it has good clinical use value.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to a strain of Pantoea disperse capable of producing bacteriocins and its screening method and application. Background technique [0002] Bacteriocin is a polypeptide, protein or protein complex with antibacterial activity produced by bacteria, generally only toxic to bacteria with close relatives, and the producing bacteria are autoimmune to the bacteriocin produced by it (generally believed, The self-immunity of bacteria is due to the presence of immune genes, and the immune mechanism may be that the immune proteins produced by immune genes shield receptor proteins, or competitively interfere with bacteriocin molecules and block pores to prevent bacteriocin from forming membrane channels). [0003] Bacteriocins come from a wide range of sources and can be produced by Gram-positive bacteria (G+), Gram-negative bacteria (G-) or some archaea. Its good characteristics of di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00A61K38/02A61P31/06C12R1/01
CPCA61K35/74A61K38/02C12N1/20C12P21/00C12N1/205C12R2001/01
Inventor 危宏平滕铁山杨航余军平
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI