Bacillus amyloliquefaciens gfj-4 and bacillus amyloliquefaciens gfj-4 containing composition

A technology of gfj-4 and amylolytic spores, which is applied in the field of microorganisms, can solve problems such as difficult biodegradation and ecological environment damage, and achieve the effects of good environmental compatibility, reduced usage, and reduced residues

Active Publication Date: 2015-04-22
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as a heterogeneous compound in the environment, chemical fungicides are difficult to biodegrade, and the damage to the ecologic

Method used

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  • Bacillus amyloliquefaciens gfj-4 and bacillus amyloliquefaciens gfj-4 containing composition
  • Bacillus amyloliquefaciens gfj-4 and bacillus amyloliquefaciens gfj-4 containing composition
  • Bacillus amyloliquefaciens gfj-4 and bacillus amyloliquefaciens gfj-4 containing composition

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Experimental program
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Effect test

Embodiment 1

[0028] Identification of embodiment 1 biocontrol strain

[0029] In this study, a bacterium gfj-4 with broad-spectrum antagonistic activity against phytopathogenic fungi was isolated from the collected diseased tomato fruits. The strain was observed through the combination of morphological observation, physiological and biochemical characteristics analysis and 16S rRNA gene sequence analysis. Identified as Bacillus amyloliquefaciens.

[0030] The isolation and purification process of strain gfj-4 is as follows:

[0031] 1) Bacteria solution preparation

[0032] Disinfect the surface of the collected diseased tomato fruits with medical cotton balls soaked in 75% alcohol, then cut the fruits with a sterile scalpel and put them in a sterile mortar to fully grind and homogenate, and then use the stepwise dilution method to homogenize the fruit. Gradiently dilute the slurry to three concentrations of 10-2, 10-3 and 10-4 for later use.

[0033] 2) Aseptic culture

[0034] First,...

Embodiment 2

[0049] The antibacterial activity of embodiment 2 bacillus amyloliquefaciens gfj-4

[0050] 1. Preparation of fermentation medium

[0051] Potato (peeled) 200.0g, glucose 18.0g, magnesium sulfate 1.0g, ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.60g, deionized water 1.0L, 16% agar strips can be added to make a solid medium for use.

[0052] 2. Preparation of inactivated fermentation broth

[0053] Cultivate Bacillus amyloliquefaciens gfj-4 strain on the ordinary medium plate for 48 hours, pick a single colony lawn and transfer it to the fermentation medium, shake culture at 33°C, 140r min-1 for 72 hours, and then put the cultured The fermentation broth was sterilized in a high-pressure steam sterilizer at 115°C for 20 minutes. Then put the inactivated fermented liquid in a centrifuge at 8000r min -1 , 15°C, centrifuge for 30min, take the supernatant and store it in a 4°C refrigerator for later use.

[0054] 3. Determination of antibacterial activity

[0055] U...

Embodiment 3

[0072] Synergistic combination of embodiment 3 inactivated fermented liquid and chemical bactericide

[0073] Adopt mycelia growth rate method to draw, the inhibition rate of 10-fold dilution of biocontrol bacterium inactivated fermented liquid reaches 50% to Fusarium graminearum mycelia growth, and 8 kinds of chemical fungicides to the growth rate of Fusarium graminearum mycelia EC50 are: the EC500.06μg / mL of fludioxonil, 0.85μg / mL of carbendazim, 0.32μg / mL of tebuconazole, 1.71μg / mL of difenoconazole and 1.80μg / mL of myclobutanil mL, thiram is 8.90 μg / mL, mancozeb is 11.16 μg / mL and triadimefon is 9.65 μg / mL.

[0074] The toxicity test design of Horsfall was used to study the inhibitory effect of the mixture of inactivated fermentation broth and chemical fungicides on Fusarium graminearum. The calculation formula of Horsfall toxicity test is as follows:

[0075] Actual bacteriostatic rate (%)=[(control colony diameter-treatment colony diameter) / (control colony diameter-bac...

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Abstract

The invention discloses bacillus amyloliquefaciens gfj-4. The bacillus amyloliquefaciens gfj-4 is collected in the China Center for Type Culture Collection (CCTCC) on September 24, 2014, and the collection number of the bacillus amyloliquefaciens gfj-4 is of CCTCC NO:M 2014444. The invention further discloses a bacillus amyloliquefaciens gfj-4 containing composition, and the bacillus amyloliquefaciens gfj-4 containing composition comprises an active component A and an active component B, wherein the weight ratio of the active component A and the active component B is 9:1 to 1:9. The active component A is the inactivated fermentation liquor of bacillus amyloliquefaciens gfj-4, and the active component B is fludioxonil, carbendazim, tebuconazole, difenoconazole, myclobutanil, thiram, mancozeb or triadimefon. The bacillus amyloliquefaciens gfj-4 containing composition can be used for preventing and treating 95% of diseases caused by fusarium graminearum, is safe to human beings and animals and has the characteristic of good environmental compatibility.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus amyloliquefaciens gfj-4 and a composition thereof. Background technique [0002] Fusarium graminearum (Fusarium graminearum) is a pathogenic bacterium of many plant diseases, which is very serious in damage and great in loss. At present, the diseases caused by this pathogen are mainly controlled by the application of chemical fungicides. However, as heterogeneous compounds in the environment, chemical fungicides are difficult to biodegrade, and the destruction of the ecological environment caused by the extensive use of chemical fungicides has increasingly attracted the attention of the government and people. Therefore, the reduced use of chemical fungicides and the development of microbial-derived natural product pesticides have become the focus of research on plant disease control. [0003] In this study, Bacillus amyloliquefaciens (Bacillus amyl...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07A01N43/36A01N47/18A01N43/653A01N47/26A01N47/14
CPCA01N43/36A01N43/653A01N47/14A01N47/18A01N47/26A01N63/10C12N1/205C12R2001/07A01N2300/00
Inventor 段海明余利
Owner ANHUI SCI & TECH UNIV
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