A kind of enzymatic chemiluminescence substrate solution

An enzyme-catalyzed chemiluminescence, substrate liquid technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve the problem of high reagent cost, inconvenient use in primary clinical hospitals, and inability to meet the high sensitivity of clinical detection. , high specificity requirements and other issues, to achieve the effects of high sensitivity, prolonged luminescence duration, and low cost

Active Publication Date: 2017-02-01
同昕生物技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The horseradish peroxidase (Horseradish Peroxidase, HRP) enzymatic chemiluminescent substrate system currently on the market in chemiluminescent in vitro diagnostic reagents mostly uses the luminol-carbamide peroxide-enhancer system, and the carbamide peroxide itself is unstable , easy to release O with prolonged storage time 2 , the plateau period of the existing luminol-enhanced system cannot meet the high sensitivity and high specificity requirements of clinical detection, and the stability of the luminescent substrate system needs to be improved. Although the imported luminescent substrate has high sensitivity and strong specificity , The platform period is long, but the reagent cost is relatively high, which is not convenient for the majority of primary clinical hospitals to use

Method used

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  • A kind of enzymatic chemiluminescence substrate solution
  • A kind of enzymatic chemiluminescence substrate solution
  • A kind of enzymatic chemiluminescence substrate solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, preparation of enzymatic chemiluminescent substrate solution 1 of the present invention

[0044] (1) Preparation of enzymatic chemiluminescent substrate solution A:

[0045] Weigh 0.0094g carbamide peroxide (Sigma Company 289132), 0.01g sodium tetrapolyphosphate (Shanghai Huikai Company 7727-67-5), 0.03g sodium fluoride (Sinopharm Reagent 10019608), 0.01g sodium tartrate (Sinopharm Reagent 30169816 ), 0.172g citric acid (C6H 8 o 7 ·H 2 O national medicine reagent 10007108), 0.347g sodium citrate (Na 3 C 6 h 5 o 7 2H 2 O National Medicine Reagent 10019428), adjust the pH to 5.0, and settle to 100ml with purified water.

[0046] The concentration of each component in the enzymatic chemiluminescent substrate solution A liquid prepared by the above method is as follows:

[0047] Carbamide peroxide, concentration: 1mM;

[0048] Sodium tetrapolyphosphate, the mass percentage concentration is: 0.01%;

[0049] Sodium fluoride, mass percent concentration...

Embodiment 2

[0061] Embodiment 2, preparation of enzymatic chemiluminescent substrate solution 2 of the present invention

[0062] (1) Preparation of enzymatic chemiluminescent substrate solution A:

[0063] Weigh 0.094g carbamide peroxide (Sigma Company 289132), 0.05g sodium tetrapolyphosphate (Shanghai Huikai Company 7727-67-5), 0.05g sodium fluoride (Sinopharm Reagent 10019608), 0.1g sodium tartrate (Sinopharm Reagent 30169816 ), 0.172g citric acid (C6H 8 o 7 ·H 2 O national medicine reagent 10007108), 0.347g sodium citrate (Na 3 C 6 h 5 o 7 2H 2 O National Medicine Reagent 10019428), adjust the pH to 5.0, and settle to 100ml with purified water.

[0064] The concentration of each component in the enzymatic chemiluminescent substrate solution A liquid prepared by the above method is as follows:

[0065] Carbamide peroxide, concentration: 10mM;

[0066] Sodium tetrapolyphosphate, the mass percentage concentration is: 0.05%;

[0067] Sodium fluoride, mass percent concentration:...

Embodiment 3

[0079] Embodiment 3, preparation of enzymatic chemiluminescent substrate solution 3 of the present invention

[0080] (1) Preparation of enzymatic chemiluminescent substrate solution A:

[0081] Weigh 0.047g carbamide peroxide (Sigma Company 289132), 0.03g sodium tetrapolyphosphate (Shanghai Huikai Company 7727-67-5), 0.04g sodium fluoride (Sinopharm Reagent 10019608), 0.06g sodium tartrate (Sinopharm Reagent 30169816 ), 0.172g citric acid (C6H 8 o 7 ·H 2 O national medicine reagent 10007108), 0.347g sodium citrate (Na 3 C 6 h 5 o 7 2H 2 O National Medicine Reagent 10019428), adjust the pH to 5.0, and settle to 100ml with purified water.

[0082] The concentration of each component in the enzymatic chemiluminescent substrate solution A liquid prepared by the above method is as follows:

[0083] Carbamide peroxide, the concentration is: 5mM;

[0084] Sodium tetrapolyphosphate, the mass percentage concentration is: 0.03%;

[0085] Sodium fluoride, mass percent concent...

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Abstract

The invention discloses enzymatic chemiluminescent substrate liquid. The enzymatic chemiluminescent substrate liquid is characterized in that the enzymatic chemiluminescent substrate liquid is composed of liquid A and liquid B; the liquid A includes oxidizing agents and stabilizers; the liquid B includes illuminating agents and reinforcing agents; the oxidizing agents are urea peroxide; the stabilizers are sodium tetraphosphate, sodium fluoride and sodium tartrate; the illuminating agents are luminol; the reinforcing agents are 3-[(3-bile amide group propyl group) dimethyl ammonium]-1-propanesulfonic acid, acetaminophen and 2,4,6 tris(dimethyl amino methyl) phenol. The enzymatic chemiluminescent substrate liquid has the advantages of being stable, high in sensitivity, long in illuminating platform stage, low in cost and the like, and is suitable for clinical hospitals as in vitro diagnosis supplementary reagents.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to an enzymatic chemiluminescence substrate solution. Background technique [0002] Chemiluminescence analysis technology is one of the modern microanalysis methods. It has the advantages of high sensitivity, wide linear range, fast detection speed and simple equipment. It has been widely used in environmental science, life science, clinical medicine and other fields, and has become the current chemical analysis field. one of the hotspots. [0003] Luminol (3-aminophthalhydrazide) is currently one of the most widely used chemiluminescent reagents, which has the advantages of easy synthesis and low cost. In alkaline solution, luminol can be oxidized by oxidizing agents (such as carbamide peroxide, H 2 o 2 etc.) oxidation, the oxidation product can absorb the energy generated in the reaction to generate an excited state of 3-aminophthalate ion, which returns to a low-energy sta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 彭京胜焦守恕李全吴凡
Owner 同昕生物技术(北京)有限公司
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