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A kind of method for in vitro tissue culture Osmunda osmanthus

A technology of tissue culture and violacea, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as difficulty in obtaining success

Inactive Publication Date: 2015-12-02
ZUNYI NORMAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As a fern plant, Osmanthus aurantiaceae (Osmanthus osmanthus) has two generations of sporophyte and gametophyte in its life history. The fertilization process of its gametophyte sexual life generation requires water as a medium, and it is very difficult to use conventional in vitro culture methods. It is difficult to achieve success. According to this characteristic of ferns, the present invention has explored a set of in vitro culture methods

Method used

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Examples

Experimental program
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Embodiment Construction

[0018] The invention is illustrated by the following examples:

[0019] Materials and Methods

[0020] Material selection

[0021] Immature green sporangia spikes at the top of the reproductive leaves of Osmunda sporophyte.

[0022] Take the maturity of Osmunda sporangium ears as follows: Maturity I is the immature sporangia ears at the top of the Osmunda spore leaves, and maturity II is the yellow-brown middle mature sporangia ears in the middle of the slightly expanded Osmunda spore leaves, maturity III It is the fully developed dark brown osmanthus mature sporangia at the bottom end of the spore leaf of Osmunda.

[0023] Induce sporophyte to germinate and form prothallium culture

[0024] The formula for inducing sporophyte germination to form prothal body nutrient base is: MS diluted a certain concentration and added different concentrations of hormones in a combined agar medium formula, sterilized by autoclave, sterilization conditions are high pressure 0.1Kpa, temperature 121℃ , ...

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Abstract

The invention relates to a method for culturing osmunda japonica by using in-vitro tissue. The method disclosed by the invention comprises the following steps of scissoring an immature green fertile spike on the top end of the fertile spike of osmunda japonica; sterilizing an explant, cutting the fertile spike of osmunda japonica into sections, and inoculating the sections in an agar culture medium, and culturing; transferring germinal young prothallus in a prothallus proliferation agar culture medium at 23-26 DEG C with the illumination of 12h / d and the intensity of 2300-2500lx; taking out proliferated prothallus, putting the proliferated prothallus in a sterile triangular flask containing a liquid culture medium, carrying out spore culture in a culture medium containing 0.1mg / L of 1 / 2MS+6-BA and 0.01 mg / L of NAA, oscillating on a shaking table for 4-10min, respectively inoculating proper amount of prothallus in a culture bottle containing a filter paper bridge or cotton, and pouring into an MS liquid culture medium and culturing; and transferring germinal sporophore in the culture bottle containing the filter paper bridge or the cotton, and pouring into a sporophore proliferation liquid culture medium, and culturing.

Description

Technical field [0001] The present invention relates to the tissue culture of pteridophytes, in particular to a method for in vitro tissue culture Osmunda japonica. Background technique [0002] Osmunda is a widely distributed fern. Its young leaves are edible. It is called "Osmunda japonica" and is a famous mountain treasure. The rhizome can be used as medicine, has the effects of clearing away heat, detoxifying, and inhibiting bacteria, and has certain application value. In recent years, natural resources have gradually depleted due to over-harvesting. In the natural environment, ferns mainly multiply through spores, or produce new plants through roots, rhizomes, leaves and other parts of asexual spores and apical meristems. However, the seedling rate of spores under natural conditions is very low, which cannot meet the needs of market and social development, and is not conducive to population expansion and artificial cultivation. The invention provides a medium formula and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 周迅
Owner ZUNYI NORMAL COLLEGE
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