Application method of rastonia solanacearum bacteriophage

Abstract

The invention discloses an application method of rastonia solanacearum bacteriophage. The application method comprises the following steps: placing rastonia solanacearum bacteriophage into a sterile syringe needle diagonally inserted in a tobacco plant stem, then covering the rastonia solanacearum bacteriophage with sterile mineral oil so as to prevent evaporation and pollution, so that the rastonia solanacearum bacteriophage directly enters the tobacco plant stem through the sterile syringe needle. According to the characteristic that the rastonia solanacearum is typical bacterial vascular bundle disease, the application method for utilizing the bacteriophage resources to prevent rastonia solanacearum disease is designed, and the method is applied to prevention and control of bacterial wilt of crops, the application amount of the rastonia solanacearum bacteriophage can be reduced, the influence of the outside factors such as sunshine, ultraviolet rays, and rainwater can be avoided, and the resistance on the bacteriophage obtained through the coevolution of the pathogen rastonia solanacearum and the rastonia solanacearum bacteriophage due to long-time contact can be avoided.

Description

technical field [0001] The invention belongs to the technical field of crop bacterial disease prevention and control, and in particular relates to an application method of Ralstonia solanacearum phage. Background technique [0002] bacterial wilt is caused by Ralstonia solanacearum (referred to as R. solanacearum for short) is a systemic disease caused by infection. It is a typical vascular disease. The most prominent symptom is wilting. When the disease is severe, it will cause the death of the whole plant and cause significant economic losses to crop production. During the planting and growth of crops, the prevention and control of bacterial wilt includes chemical pesticide control, reasonable crop rotation such as corn rotation, planting disease-resistant varieties, transplanting in advance to avoid the peak period of bacterial wilt, adjusting soil microbial structure, and applying functional organisms. Comprehensive measures such as organic fertilizers are used to preve...

AI Technical Summary

Problems solved by technology

[0002] bacterial wilt is caused by Ralstonia solanacearum (referred to as R. solanacearum for short) is a systemic disease caused by infection. It is a typical vascular disease. The most prominent symptom is wilting. When the disease is severe, it will cause the death of the whole plant and cause significant economic losses to crop production.

In the process of planting and growing crops, the prevention and control of bacterial wilt includes chemical agent control, reasonable crop rotation such as corn rotation, planting disease-resistant varieties, transplanting in advance to avoid the peak period of bacterial wilt, adjusting soil microbial structure, and applying functional organisms. Comprehensive measures such as organic fertilizers are used to prevent and control bacterial wilt, but they are still not completely effective in preventing and controlling the occurrence of bacterial wilt

The R. solanacearum phage with specificity and lysis to R. solanacearum can control the number of R. solanacearum in soil or plants, reduce the pathogenicity of R. solanacearum, and has good biological control of R. solanacearum Application prospects, but the application of phage is affected by many environmental factors such as ultraviolet rays, sunlight, rain and other factors