Sugar-reducing application of citrus changshanensis extract

A kind of extract, Huyou technology, applied in the field of medicine

Inactive Publication Date: 2015-04-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on whether Huyou extract has hypoglycemic activity in vivo

Method used

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  • Sugar-reducing application of citrus changshanensis extract
  • Sugar-reducing application of citrus changshanensis extract
  • Sugar-reducing application of citrus changshanensis extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of Huyou Extract

[0018] Weigh 1 g freeze-dried powder of Huyou oil cell layer, white skin layer and capsular coating, and use 20 mL of 80% acetone (solid-liquid ratio 1:20) to ultrasound for 30 min, frequency 60 kHz, power 30 W, extract The clarified extract was obtained by suction filtration under reduced pressure. This was repeated 3 times, the extracts were combined, and the acetone was evaporated on a rotary evaporator to obtain an aqueous solution of Huyou extract.

[0019] Solid Phase Extraction (SPE) Sep-Pak for Aqueous Solution ® Purification on C18 column (12 cc / 2 g, Waters), the purification process includes five steps: activation, equilibration, loading, elution and elution: Sep-Pak ® The C18 column is first activated with 20 mL methanol, and then equilibrated with 20 mL deionized water, and then the aqueous solution is loaded. After sufficient adsorption, the cartridge is rinsed with 40-80 mL deionized water to wash away the sugar and...

Embodiment 2

[0020] Example 2 Huyou extract C57BL / 6 Mouse test

[0021] C57BL / 6 mice were purchased from Shanghai Slack Laboratory Animal Co., Ltd., and the low-fat diet (containing 10% fat) and high-fat diet (containing 45% fat) were purchased from Jiangsu Medisen Biopharmaceutical Co., Ltd. All mice were raised in a constant temperature and humidity environment with 12 hours of light and 12 hours of darkness. There were 10 mice in each group. The low-fat+clear water group was a low-fat diet group, and the high-fat+clear water group was a high-fat diet-induced diabetes group. The doses of the three treatment groups were 40 mg / kg BW. All mice can freely ingest food and distilled water. The treatment group was administered by gavage with a needle, the gavage volume was 20 mL / kg BW, and the low-fat and high-fat control groups were gavaged with distilled water. The trial lasted for 16 weeks. The drug was administered for 6 consecutive days in a week, and the drug was stopped for 1 day. T...

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PUM

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Abstract

The invention provides an application of citrus changshanensis extract in preparing sugar-reducing functional foods, health-care products or sugar-reducing medicines. The citrus changshanensis extract comprises an oil-cell layer, a white-skin layer and a bag-coating extract, so that the fasting blood glucose and the serum glucose of a C57BL / 6 mouse with diabetes can be obviously reduced in vivo, the glucose tolerance can be increased and the insulin resistance can be reduced. The citrus changshanensis extract can be used for preventing and treating diseases related to abnormal glucose metabolism as a functional food, a health-care product or a preventing and treating medicine.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of the grapefruit extract from natural sources in the preparation of hypoglycemic functional food, health products or prevention and treatment drugs, in particular to the application of the grapefruit extract to reduce blood sugar in diabetic C57BL / 6 mice. Background technique [0002] Diabetes is an epidemic characterized by high blood sugar in the body. There are two types of diabetes: type 1 diabetes (insulin-dependent) and type 2 diabetes (non-insulin-dependent), with genetic and environmental factors contributing to both. The development of diabetes is often accompanied by other metabolic syndromes such as obesity, hyperlipidemia and inflammation. In the past few decades, the incidence of diabetes and its complications has increased significantly. According to the World Health Organization (WHO), there are 347 million diabetics worldwide. Type 2 diabetes accounts for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A61P3/10A23L1/29A23L33/00
Inventor 李鲜贾胜孙崇德陈昆松
Owner ZHEJIANG UNIV
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