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Near-infrared long persistence/down-conversion fluorescence bimodal biological imaging material and preparation method thereof

A biological imaging, dual-mode technology, applied in the direction of luminescent materials, chemical instruments and methods, pharmaceutical formulations, etc., can solve the problems of only a few days or a few hours, poor imaging effect, weak luminous intensity of afterglow materials, etc., to achieve good imaging effect Effect

Active Publication Date: 2015-04-29
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although long-lasting materials have irreplaceable advantages as fluorescent probes, and their application in biological labeling has been extensively studied, but this material also faces an unavoidable problem, that is, the luminous intensity decays with time.
Especially considering the application of fluorescent nano-markers with targeting function, after tracking the markers in the early stage, most of the markers have been targeted and bound to cancer cells, and the observation at this time can be performed by distributed detection Converted to specific organ detection, and the luminous intensity of the long-lasting afterglow material is already very weak at this time, and the imaging effect is poor
Generally speaking, the afterglow time of long-persistence materials is only a few days or a few hours, especially after being made into nano-markers, the time available for effective detection is shorter, while for in vivo imaging, especially cell tracking, it usually takes many days or even a few hours. Weeks, so general long afterglow materials can't meet this demand at all

Method used

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  • Near-infrared long persistence/down-conversion fluorescence bimodal biological imaging material and preparation method thereof
  • Near-infrared long persistence/down-conversion fluorescence bimodal biological imaging material and preparation method thereof
  • Near-infrared long persistence/down-conversion fluorescence bimodal biological imaging material and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] According to the following composition: matrix La 3 Ga 5 GeO 14 , Cr 3+ The doping amount is 0.5mol% of the matrix, Nd 3+ The doping amount is 0.5mol% of the matrix. Select compounds containing lanthanum oxide, gallium oxide, germanium oxide, chromium oxide, and neodymium oxide respectively, weigh them according to the stoichiometric ratio, and grind them evenly in a mortar; the uniformly ground raw materials are pre-calcined at 1000°C for 4 hours and then taken out. After grinding again, it was fired at 1350°C for 4h. In order to prove the feasibility of the scheme, we synthesized three comparative samples of different components: (1).La 3 Ga 5 GeO 14 :0.005Nd 3+ (No. 1 sample), (2).La 3 Ga 5 GeO 14 :0.005Cr 3+ (No. 2 sample), (3).La 3 Ga 5 GeO 14 :0.005Cr 3+ ,0.005Nd 3+ (No. 3 sample).

[0034] The long afterglow emission spectrum ( figure 1) and attenuation spectrum ( figure 2 ), proving that Nd 3+ The addition of not only did not weaken the ori...

Embodiment 2

[0036] According to the following composition: Ca 3 Ga 2 Ge 3 o 12 , Cr 3+ The doping amount is 5mol% of the matrix, Nd 3+ The doping amount is 10mol% of the matrix, and the compounds containing calcium carbonate, gallium nitrate, germanium oxide, chromium fluoride, and neodymium fluoride are respectively selected, weighed according to the stoichiometric ratio, and fully ground in a mortar; The final raw material was calcined at 1000°C for 6 hours, then taken out, ground again, and fired at 1550°C for 2 hours. After testing the afterglow spectrum, the emission peak is located at 700nm ( Figure 5 ), the excitation wavelength of the fluorescence spectrum is 808nm, and the emission wavelength is 1064nm ( Image 6 ).

Embodiment 3

[0038] According to the following composition: Zn 3 Ga 2 Ge 2 o 10 , Cr 3+ The doping amount is 0.1mol%, Nd 3+ The doping amount is 0.1mol%. Select the compounds containing zinc oxide, gallium fluoride, germanium oxide, chromium carbonate, and neodymium oxide respectively, weigh them according to the stoichiometric ratio, and grind them thoroughly in a mortar; the uniformly ground raw materials are pre-fired at 600°C for 2 hours and then taken out , After grinding again, it was fired at 1250°C for 6h. After testing the afterglow spectrum, the emission peak is located at 702nm ( Figure 7 ), the excitation wavelength of the fluorescence spectrum is 810nm, and the emission wavelength is 1065nm ( Figure 8 ).

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Abstract

The invention discloses a near-infrared long persistence / down-conversion fluorescence bimodal biological imaging material. The base material is La3Ga5GeO14, Zn3Ga2Ge2O10 or Ca3Ga2Ge3O12, and 0.1-5 mol% of Cr<3+> and 0.1-10 mol% of Nd<3+> are doped in the base material. The invention also discloses a preparation method of the material. The material can emit 700nm near-infrared long persistence under the irradiation of an ultraviolet lamp; and when the afterglow intensity becomes weak, the near-infrared light source can be used as exciting light to detect the near-infrared emitted light of the down-conversion fluorescence to further excite the imaging material injected into the body, thereby implementing biological in-vivo imaging and cell tracing later-period observation.

Description

technical field [0001] The invention relates to a biological imaging material, in particular to a dual-mode biological imaging material with near-infrared long afterglow and down-conversion fluorescence and a preparation method. Background technique [0002] With the development of biomedical research, people's research on the nature of various life phenomena and processes has become more and more in-depth, and related science and technology have also developed accordingly. More representative biological imaging techniques include: optical imaging, nuclear magnetic resonance imaging, ultrasound imaging, X-ray computed tomography, etc. Among them, optical imaging technology is widely used in this kind of research because of its advantages of strong maturity and convenient use. Optical imaging uses photons as the information source, and the imaging objects are usually cells and tissues. In recent years, with the development of science and technology, its research objects have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/80C09K11/66A61K49/00
Inventor 邱建荣吴溢铃李杨
Owner SOUTH CHINA UNIV OF TECH
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