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Detection method and device for whitening and freckle-removing components in cosmetics

A technology of whitening and freckle-removing and detection method, applied in the field of detection, can solve the problem that the whitening and freckle-removing ingredients cannot be detected at the same time.

Inactive Publication Date: 2015-04-29
FOOD INSPECTION CENT OF CIQ SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current detection of whitening and freckle-removing ingredients in cosmetics mainly focuses on the detection of banned whitening and freckle-removing ingredients, such as phenol, hydroquinone and kojic acid, and a small part can be used to determine arbutin, ascorbic acid and its derivatives, etc., but cannot Simultaneously tested all existing whitening and anti-spotty ingredients namely Magnesium Ascorbyl Phosphate, Kojic Acid, Hydroquinone, Niacinamide, Raspberry Glycerin, Phenol, Arbutin and Dipotassium Glycyrrhizinate

Method used

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  • Detection method and device for whitening and freckle-removing components in cosmetics
  • Detection method and device for whitening and freckle-removing components in cosmetics
  • Detection method and device for whitening and freckle-removing components in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Establishment of standard curves for eight whitening and freckle-removing ingredients

[0072] Accurately weigh 200 mg each of magnesium ascorbyl phosphate (99.0%), kojic acid (99.9%), hydroquinone (99.0%), nicotinamide (99.9%), raspberry glycoside (99.1%), and phenol (99.5%), bear Fruit glycosides (96.6%) 207mg, dipotassium glycyrrhizinate (83.1%) 241mg were dissolved in water and transferred to 100mL volumetric flasks respectively, and the volume was adjusted to the mark to prepare a standard stock solution. Pipette 5.0 mL each of standard stock solutions of magnesium ascorbyl phosphate, nicotinamide, and phenol, 10.0 mL each of standard stock solutions of arbutin, hydroquinone, raspberry, and dipotassium glycyrrhizinate, and 3.0 mL to 100 mL of standard stock solutions of kojic acid In the volumetric flask, dilute to the mark with potassium dihydrogen phosphate solution (0.02mol / L, pH=6.95), and then dilute to 5.0, 10.0, 20.0, 50.0, 100.0 mg / L; 10.0, 20.0, 50.0, 100....

Embodiment 2

[0084] Detection of whitening and freckle-removing ingredients in cosmetics

[0085] (1) Sample handling

[0086] Take about 0.500g of the cosmetic to be tested in a 50mL stoppered colorimetric tube, add 20mL of potassium dihydrogen phosphate solution (0.02mol / L, pH=6.95), ultrasonically extract for 20min, transfer to a 50mL volumetric flask, and set the volume to the mark. After mixing, take 10mL of the solution in a 15mL centrifuge tube, centrifuge at 12000r / min for 10min, pass the supernatant through a 0.22μm filter membrane, and the filtrate is the extract solution, to be determined. The cosmetics to be tested are moisturizing lotion, whitening cream, whitening gel, moisturizing mask and other varieties.

[0087] (2) Chromatographic conditions

[0088] High-performance liquid chromatography with a diode array detector (Agilent 1100, Agilent, USA);

[0089] Chromatographic column: Zorbax Eclipse XDB-C18, 250mm×4.6mm, 5.0μm;

[0090] Mobile phase: A potassium dihydrogen ...

Embodiment 3

[0108] Determination of 8 kinds of whitening and freckle-removing ingredients in the cosmetics to be tested with different concentrations of whitening and freckle-removing ingredients

[0109] (1) Sample handling

[0110] Using the liquid, cream and gel cosmetics to be tested from the same source as in Example 2 as a blank matrix, prepare whitening products containing three different concentrations of 1 times the lower limit of detection, 2 times the lower limit of detection, and 5 times the lower limit of detection Cosmetic sample of freckle remover.

[0111] Accurately weigh about 0.500g of the cosmetic to be tested into a 50mL stoppered colorimetric tube, add 20mL of potassium dihydrogen phosphate solution (0.02mol / L, pH=6.95), ultrasonically extract for 20min, transfer to a 50mL volumetric flask, and dilute to scale. After mixing, take 10mL of the solution in a 15mL centrifuge tube, centrifuge at 12000r / min for 10min, pass the supernatant through a 0.22μm filter membrane...

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Abstract

The invention relates to a detection method and device for the whitening and freckle-removing components in cosmetics. The detection method comprises the following steps of: adding the cosmetics to be detected into buffer salt solution, oscillating and extracting to obtain corresponding extracting solution, wherein the concentration of the buffer salt solution is 0.01-0.03mol / L, and the volume of the buffer salt solution corresponding to every gram of cosmetics to be detected is 40-100mL; layering the extracting solution to take supernatant liquor, and filtering the supernatant liquor to obtain extract solution; adopting hydrophilic organic solution and the buffer salt solution as a mobile phase to carry out chromatographic analysis on the extract solution to obtain a chromatogram; obtaining the content of the whitening and freckle-removing components in the cosmetics to be detected by operation according to the chromatogram and a pre-drawn standard curve, wherein the standard curve corresponds to magnesium ascorbyl phosphate, kojic acid, hydroquinone, nicotinamide, raspberry ketone glucoside, phenol, arbutin and dipotassium glycyrrhizinate. The detection method and device have the advantage that the simplicity and the efficiency of detection are greatly improved.

Description

technical field [0001] The invention relates to the detection field, in particular to a detection method and device for whitening and freckle-removing ingredients in cosmetics. Background technique [0002] With the development of various external skin whitening treatments, most skin whitening cosmetics use more than one whitening and freckle-removing ingredient. Effective control of skin darkening requires effective control of each link, that is, the "synergistic effect" theory of whitening and freckle removal. [0003] The cross-use of multiple whitening and freckle-removing ingredients will have safety problems, especially when new whitening and freckle-removing ingredients come out at random times, and there is a lack of reliable efficacy and toxicological information. It has been suggested that the cross-use and excessive use of whitening and freckle-removing ingredients will interfere with the normal enzymatic conversion of tyrosine to melanin in the skin, thereby cau...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 陈沛金涂小珂肖锋
Owner FOOD INSPECTION CENT OF CIQ SHENZHEN
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