Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Zebrafish vitellogenin indirect competition ELISA kit and its detection method and application

A technology of original protein and kit, applied in the field of zebrafish vitellogenin indirect competition ELISA kit and its detection, to achieve the effect of reducing induction time and workload, low price and reducing workload

Inactive Publication Date: 2016-06-08
OCEAN UNIV OF CHINA
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Chinese patent 201210559592.1 provides a kit for the qualitative detection of fish vitellogenin using vitellin phosphoprotein antibody, which has a significant detection effect, but the kit can only qualitatively detect zebrafish vitellogenin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Zebrafish vitellogenin indirect competition ELISA kit and its detection method and application
  • Zebrafish vitellogenin indirect competition ELISA kit and its detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] A sandwich ELISA kit for detecting zebrafish vitellogenin, including a box body, a blank 96-well microtiter plate, coating solution, blocking solution, sample diluent, washing solution, chromogenic solution, stop solution and spicy One goat anti-rabbit secondary antibody labeled with root peroxidase,

[0032] It is characterized in that the box body is also equipped with: one pure zebrafish yolk lipophosphoprotein and one rabbit anti-zebrafish vitellogenin polyclonal antibody.

[0033] The preparation of a sandwich ELISA kit for detecting zebrafish vitellogenin includes the following steps:

[0034] (1) preparing pure zebrafish yolk lipophosphoprotein;

[0035] (2) preparing rabbit anti-zebrafish vitellogenin polyclonal antibody;

[0036] Wherein, the (1) method for preparing pure zebrafish yolk lipophosphoprotein is as follows:

[0037] Weigh the zebrafish ovary after taking it out, add 3 times the volume of 4°C pre-cooled PBS buffer (containing 1mMPMSF, pH 7.4) to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a zebra fish vitellogenin indirect competition ELISA kit and a detection method and application of the kit. The method comprises the following steps: respectively purifying lipovitellin and vitellogenin from zebra fish ovary and 17beta-estradiol exposed zebra fish body by using a method for combining two-step chromatography and ultrafiltration, and preparing rabbit anti-zebra fish vitellogenin polyclonal antibody; respectively filling a pure zebra fish lipovitellin product, the rabbit anti-zebra fish vitellogenin polyclonal antibody, a blank 96-pore ELISA plate, a coating solution, a washing solution, a blocking solution, sample diluent, a color developing solution, a stopping solution and a horse radish peroxidase labeled goat anti-rabbit second antibody into a box body, thereby obtaining the indirect competition ELISA kit for detecting the zebra fish vitellogenin. The kit disclosed by the invention can be applied to investigation of environmental estrogen substances, the vitellogenin in zebra fish plasma, overall homogenate, liver tissues and hepatic cell culture solution can be sensitively, accurately, conveniently and quantitatively detected, the detection range is 31.25-1000ng mL<-1>, and an important tool is provided for the application of the zebra fish in the field of endocrine disrupter detection.

Description

technical field [0001] The invention belongs to the field of environmental detection, and in particular relates to a zebrafish vitellogenin indirect competition ELISA kit and a detection method and application thereof. Background technique [0002] With the continuous improvement of the degree of industrialization and the living standards of the people in our country, a large amount of industrial wastewater and domestic sewage are discharged into rivers, lakes and seas, causing serious water environmental pollution, which not only endangers the survival of wild creatures, but also seriously threatens the life of our people. healthy. Although the concentration of these pollutants in the environment is very low, they can interfere with a series of biological processes such as the synthesis, release, transportation, and metabolism of hormones in humans and animals, as well as the combination of hormones and receptors, and the expression of functions, thereby disrupting human an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/531G01N33/68
Inventor 汝少国王军王蔚田华
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com