Method for simultaneously determining three kinds of phenylethanoid glycoside compositions in callicarpa nudiflora preparation through HPLC

A technology of nudiflora and phenylethanol glycosides, which is applied in measuring devices, material separation, and analysis of materials, can solve the problems of cumbersome sample processing, achieve good reproducibility, ensure clinical efficacy, and high precision

Active Publication Date: 2015-05-06
JIUZHITANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

"Determination of Acteoside and Forsythiaside in Nudehua Zizhu Tablets by HPLC Method" by Chen Dejin et al., published in "Chinese Patent Medicine" in March 2011, the sample processing is cumbersome, and the main component isobracoside was not detected.

Method used

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  • Method for simultaneously determining three kinds of phenylethanoid glycoside compositions in callicarpa nudiflora preparation through HPLC
  • Method for simultaneously determining three kinds of phenylethanoid glycoside compositions in callicarpa nudiflora preparation through HPLC
  • Method for simultaneously determining three kinds of phenylethanoid glycoside compositions in callicarpa nudiflora preparation through HPLC

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Experimental program
Comparison scheme
Effect test

Embodiment 2

[0093] Embodiment 2 Method for simultaneous determination of forsythoside B, verbascoside, and isorrascoside in Nuihua Zizhu Capsules

[0094] 1.1 Drugs and Reagents

[0095] Reference substances Forsythoside B (batch number: 111811-201001, for content determination, China National Institute for Food and Drug Control), Verbasin (batch number: 111530-201208, for content determination, China Institute for Food and Drug Control), isoverbasin (Batch No.: Y-073-121023, for content determination, Chengdu Ruifensi Biotechnology Co., Ltd.); Nude Flower Zizhu Capsule: 0.4g / capsule, batch No. 20080801, 20081102, 20090302, commercially available, Jiangxi Xinglin Baima Medicine Industry Co., Ltd. Acetonitrile was chromatographically pure (TEDA, USA), water was ultrapure water, and other reagents were of analytical grade.

[0096] 1.2 Instruments

[0097] Agilent1200 high performance liquid chromatograph; KQ3200DE CNC ultrasonic cleaner (Kunshan Instrument Co., Ltd.); Milli-Q pure water...

Embodiment 3

[0109]Embodiment 3 Simultaneous determination of forsythoside B, verbascoside, and isoverascoside in Nuihua Zizhu Granules

[0110] 1.1 Drugs and Reagents

[0111] Reference substances Forsythoside B (batch number: 111811-201001, for content determination, China National Institute for Food and Drug Control), Verbasin (batch number: 111530-201208, for content determination, China Institute for Food and Drug Control), isoverbasin (Batch No.: Y-073-121023, for content determination, Chengdu Refensi Biotechnology Co., Ltd.); Nude Flower Zizhu Granules: 3g / bag, batch Nos. 20110305, 20110306, 20110307, ​​provided by Hainan Jiuzhitang Pharmaceutical Co., Ltd. . Acetonitrile was chromatographically pure (TEDA, USA), water was ultrapure water, and other reagents were of analytical grade.

[0112] 1.2 Instruments

[0113] Agilent1200 high performance liquid chromatograph; KQ 3200 DE type CNC ultrasonic cleaner (Kunshan Instrument Co., Ltd.); Milli-Q pure water system (Millipore, USA)...

Embodiment 4

[0125] Embodiment 4 Simultaneous determination of forsythoside B, verbascoside, and isorrascoside in Nude Flower Zizhu Mixture

[0126] 1.1 Drugs and Reagents

[0127] Reference substances Forsythoside B (batch number: 111811-201001, for content determination, China National Institute for Food and Drug Control), Verbasin (batch number: 111530-201208, for content determination, China Institute for Food and Drug Control), isoverbasin (Batch No.: Y-073-121023, for content determination, Chengdu Ruifensi Biotechnology Co., Ltd.); Naked Flower Zizhu Mixture: 200ml / bottle, batch Nos. 20110812, 20110813, 20110814, provided by Hainan Jiuzhitang Pharmaceutical Co., Ltd. . Acetonitrile was chromatographically pure (TEDA, USA), water was ultrapure water, and other reagents were of analytical grade.

[0128] 1.2 Instruments

[0129] Agilent1200 high performance liquid chromatograph; KQ3200DE CNC ultrasonic cleaner (Kunshan Instrument Co., Ltd.); Milli-Q pure water system (Millipore, US...

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Abstract

The invention discloses a method for simultaneously determining three kinds of phenylethanoid glycoside compositions in a callicarpa nudiflora preparation through HPLC. The method comprises: (1) preparation of a contrast sample solution; (2) preparation of a to-be tested sample solution; and (3) HPLC detection. The method is simple in operation, high in accuracy, good in specificity, relatively high in precision and good in reappearance, and is beneficial for guaranteeing stable quality of the callicarpa nudiflora preparation and clinic curative effect.

Description

technical field [0001] The invention belongs to the technical field of drug detection, and in particular relates to a method for simultaneously measuring three phenethyl glycosides in a preparation of Viola nudiflora. Background technique [0002] Nude flower Zizhu preparations such as Nude flower Zizhu Tablets, Nude Flower Zizhu Suppositories, Nude Flower Zizhu Dispersible Tablets, etc., have the functions of analgesia, anti-inflammatory, detoxification, astringency and hemostasis, etc. The efficacy is the joint action of a variety of active ingredients As a result, the current methods for the detection of Nudehuazizhu preparations such as Nudehuazizhu tablets are mainly to measure the content of total flavonoids by spectrophotometry, and to measure the index components such as luteolin, verbascoside, and forsythiaside by high performance liquid phase method. Wait. Studies on the chemical constituents of the genus Viola show that phenylethanoid glycosides are one of the ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 李典鹏卢凤来颜小捷谷陟欣黄胜张妮瑜袁莉任洁芳
Owner JIUZHITANG
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