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Determination method of refined decoction pieces of mulberry branches

A technology of refined decoction pieces and determination methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problems of not being able to reflect the quality of the refined decoction pieces of mulberry branches and unable to monitor the quality of the refined decoction pieces of mulberry branches

Active Publication Date: 2016-08-24
湖南省中医药研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Without quantitative quality control indicators, it is impossible to monitor the internal quality of refined mulberry branch pieces, nor can it reflect the quality of refined mulberry branch pieces

Method used

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  • Determination method of refined decoction pieces of mulberry branches
  • Determination method of refined decoction pieces of mulberry branches
  • Determination method of refined decoction pieces of mulberry branches

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] The content of mulberryside A and oxidized resveratrol in refined decoction pieces of mulberry branches was determined by high performance liquid chromatography. The operation method is as follows:

[0094] Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel was used as filler; 0.2% phosphoric acid solution was used as mobile phase A, acetonitrile-0.2% phosphoric acid solution was used as mobile phase B, and the gradient elution was carried out in the following table; The ratio of mobile phase B is 75~83:17~25, the detection wavelength is 325~333nm; the column temperature is 30°C, and the number of theoretical plates should not be less than 4000 based on the calculation of the molaritin A peak.

[0095] Table 14 Mobile phase gradient

[0096]

[0097] Preparation of reference solution A mixed solution of 20-25 μg of alcohol is ready.

[0098] Preparation of the test solution Take the powder of this product (passed through a No...

Embodiment 2

[0101] The content of mulberryside A and oxidized resveratrol in refined decoction pieces of mulberry branches was determined by high performance liquid chromatography. The operation method is as follows:

[0102] Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel was used as filler; 0.2% phosphoric acid solution was used as mobile phase A, acetonitrile-0.2% phosphoric acid solution was used as mobile phase B, and the gradient elution was carried out in the following table; The ratio of mobile phase B is 78~85:15~22, the detection wavelength is 327~335nm; the column temperature is 30°C, and the number of theoretical plates should not be less than 4000 based on the calculation of the mulberry side A peak.

[0103] Table 15 Mobile phase gradient

[0104]

[0105] Preparation of Reference Substance Solution: Take the appropriate amount of the reference substance of molaritin A and the reference substance of oxidized resveratrol, weigh th...

Embodiment 3

[0109] The content of mulberryside A and oxidized resveratrol in refined decoction pieces of mulberry branches was determined by high performance liquid chromatography. The operation method is as follows:

[0110] Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel was used as filler; 0.2% phosphoric acid solution was used as mobile phase A, acetonitrile-0.2% phosphoric acid solution was used as mobile phase B, and the gradient elution was carried out in the following table; The ratio of mobile phase B is 80:20, the detection wavelength is 330nm; the column temperature is 30°C, and the number of theoretical plates should not be less than 4000 based on the calculation of the molaritin A peak.

[0111] Table 16 Mobile phase gradient

[0112]

[0113] Preparation of reference solution Mix the solution, that is.

[0114] Preparation of the test solution Take the powder of this product (passed through a No. 4 sieve), take about 0.5g, accu...

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Abstract

The invention discloses a method for measuring content of a refined ramulus mori decoction piece. The method comprises the following steps: measuring content of mulberroside A and oxyresveratrol in the refined ramulus mori decoction piece through an efficient liquid-phase chromatography. By optimizing chromatographic conditions and a preparation method for a test article, inherent quality indexes of the refined ramulus mori decoction piece are precisely measured to provide guidance for clinical medication and provide a basis for drawing up content standards of the mulberroside A and oxyresveratrol. The measuring method is accurate, simple, and good in repeatability. By optimizing a mobile phase system, main index components are separated very well and high in column efficiency; the content of active ingredients of the ramulus mori and the quality of the refined ramulus mori decoction piece can be controlled through specific indexes.

Description

technical field [0001] The invention relates to a method for detecting medicines, in particular to a method for determining the content of refined decoction pieces of mulberry branches. Background technique [0002] Sangzhi is the general term for the leaves, branches, and tender branches of mulberry trees. The refined decoction pieces of mulberry branches are selected from high-quality and authentic medicinal materials, carefully processed, and selected in several specifications packaged for clinical use. The main functions are dispelling wind and dredging collaterals, promoting qi to reduce swelling, and lowering blood pressure. Sangzhi is recorded in the "Chinese Pharmacopoeia" (2010 edition), which stipulates the characteristics, microscopic identification, and inspection of extracts, but there is no quantitative index for content determination. Without quantitative quality control indicators, it is impossible to monitor the internal quality of the refined mulberry bran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/88
Inventor 黄惠勇谢谊秦裕辉张水寒肖娟
Owner 湖南省中医药研究院
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