Determination method of refined decoction pieces of mulberry branches
A technology of refined decoction pieces and determination methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problems of not being able to reflect the quality of the refined decoction pieces of mulberry branches and unable to monitor the quality of the refined decoction pieces of mulberry branches
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Embodiment 1
[0093] The content of mulberryside A and oxidized resveratrol in refined decoction pieces of mulberry branches was determined by high performance liquid chromatography. The operation method is as follows:
[0094] Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel was used as filler; 0.2% phosphoric acid solution was used as mobile phase A, acetonitrile-0.2% phosphoric acid solution was used as mobile phase B, and the gradient elution was carried out in the following table; The ratio of mobile phase B is 75~83:17~25, the detection wavelength is 325~333nm; the column temperature is 30°C, and the number of theoretical plates should not be less than 4000 based on the calculation of the molaritin A peak.
[0095] Table 14 Mobile phase gradient
[0096]
[0097] Preparation of reference solution A mixed solution of 20-25 μg of alcohol is ready.
[0098] Preparation of the test solution Take the powder of this product (passed through a No...
Embodiment 2
[0101] The content of mulberryside A and oxidized resveratrol in refined decoction pieces of mulberry branches was determined by high performance liquid chromatography. The operation method is as follows:
[0102] Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel was used as filler; 0.2% phosphoric acid solution was used as mobile phase A, acetonitrile-0.2% phosphoric acid solution was used as mobile phase B, and the gradient elution was carried out in the following table; The ratio of mobile phase B is 78~85:15~22, the detection wavelength is 327~335nm; the column temperature is 30°C, and the number of theoretical plates should not be less than 4000 based on the calculation of the mulberry side A peak.
[0103] Table 15 Mobile phase gradient
[0104]
[0105] Preparation of Reference Substance Solution: Take the appropriate amount of the reference substance of molaritin A and the reference substance of oxidized resveratrol, weigh th...
Embodiment 3
[0109] The content of mulberryside A and oxidized resveratrol in refined decoction pieces of mulberry branches was determined by high performance liquid chromatography. The operation method is as follows:
[0110] Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel was used as filler; 0.2% phosphoric acid solution was used as mobile phase A, acetonitrile-0.2% phosphoric acid solution was used as mobile phase B, and the gradient elution was carried out in the following table; The ratio of mobile phase B is 80:20, the detection wavelength is 330nm; the column temperature is 30°C, and the number of theoretical plates should not be less than 4000 based on the calculation of the molaritin A peak.
[0111] Table 16 Mobile phase gradient
[0112]
[0113] Preparation of reference solution Mix the solution, that is.
[0114] Preparation of the test solution Take the powder of this product (passed through a No. 4 sieve), take about 0.5g, accu...
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