Method for preparing MT-based solid culture medium in large scale

Abstract

The invention discloses a method for preparing an MT-based solid culture medium in a large scale and belongs to the technical field of plant biology. The method for preparing the MT-based solid culture medium in the large scale comprises the following steps: firstly calculating demanded quantities of sixteen components contained in the MT-based culture medium after being expanded by 1000 times; accurately weighing expanded quantities of medicaments, wherein CuSO4.5H2O and CoCl2.6H2O are accurately weighed by utilizing a ten-thousandth balance; successively mixing the sixteen components according to gradually increasing demanded quantities, namely the two components with the lowest demanded quantities are mixed firstly, then the component with the next-to-the-lowest demanded quantity is added, uniform mixing is carried out, and the process is repeated; sealing every 250g of solid culture medium in one fixed plastic container; and opening the plastic containers respectively as required during application. The method for preparing the MT-based solid culture medium in the large scale has the advantages that the MT-based solid culture medium expanded by 1000 times can be prepared, the characteristics of easiness and speediness in operation, simplicity in application, small error in different batches and small intra-batch error can be realized, and the phenomena, such as big error, precipitation and moulding, which are caused by manual operations such as calculating and weighing and external factors such as weather, temperature and moisture during preparation of MT liquid culture medium can be avoided, so that the method for preparing the MT-based solid culture medium in the large scale can be applied to biotechnical uses such as plant tissue culture.

Description

technical field [0001] The invention discloses a large-scale production method of MT basic solid culture substrate, which belongs to the field of plant biotechnology. More specifically, it is a five-step process including accurate calculation, accurate weighing, mixing less and more, sealing, and unsealing, in order to obtain culture substrates with minimal errors between different batches and batches, and reduce Method of trial and error. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in in...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters