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Application of chlorogenic acid in preparation of medicines for treating osteopetrosis

A technology of osteosclerosis and chlorogenic acid, applied in bone diseases, drug combinations, pharmaceutical formulations, etc., can solve the problems of poor treatment effect, large toxic and side effects, no reports of chlorogenic acid osteosclerosis, etc., and achieve clinical results. Good application prospect and less side effects

Active Publication Date: 2015-05-13
SICHUAN JIUZHANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the only effective treatment for osteopetrosis is hematopoietic stem cell transplantation, but it is very difficult to find donors for hematopoietic stem cell transplantation
Based on the difficulty of treatment and the ambiguity of the pathogenesis, usually a combination of symptomatic treatment is used, such as prednisone for anemia and Shujing Huoxue Tablets for bone damage, but the treatment effect is not good, and there are toxic side effects For example, prednisone can cause femoral head necrosis, stress ulcers, steroid-induced hyperglycemia, easy infection and mental disorders, etc.
[0004] There is no report of chlorogenic acid treating osteosclerosis

Method used

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  • Application of chlorogenic acid in preparation of medicines for treating osteopetrosis
  • Application of chlorogenic acid in preparation of medicines for treating osteopetrosis
  • Application of chlorogenic acid in preparation of medicines for treating osteopetrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: In vivo pharmacodynamics study of chlorogenic acid in the treatment of osteosclerosis

[0017] 1. Experimental materials

[0018] 1.1 Animals

[0019] Fifty BALB / C mice with osteopetrosis.

[0020] The animal model used in this example is directly purchased BALB / C mice with osteopetrosis (this mouse is also a commonly used model for basic research on osteopetrosis at home and abroad). In this mouse model, due to the abnormal growth of the bone in the sick mouse, the bone is hard, the bone marrow cavity disappears, all the bones are hardened, and there is edentulousness. The lesions of this mouse are similar to human osteosarcoma.

[0021] 1.2 Experimental Drugs and Instruments

[0022] Chlorogenic acid, prednisone (Hebei Xinyinhe Chemical Co., Ltd.), Shujin Huoxue Tablets (Henan Zhongjie Pharmaceutical Co., Ltd.), double distilled water, 12-well sterile plate, 96-well sterile plate, high power microscope, Electronic balance (one hundred thousandth).

[...

Embodiment 2

[0058] Example 2: Effect of chlorogenic acid on the expression of CA2 gene and its corresponding type II carbonic anhydrase in osteoclasts of a mouse model of osteosclerosis.

[0059] 1. Experimental materials

[0060] 1.1 Animals

[0061] Thirty BALB / C mice with osteopetrosis.

[0062] The animal model used in this example is directly purchased BALB / C mice with osteopetrosis. In this mouse model, due to the abnormal growth of the bone in the sick mouse, the bone is hard, the bone marrow cavity disappears, all the bones are hardened, and there is edentulousness. The lesions of this mouse are similar to human osteosarcoma.

[0063] 1.2 Experimental Drugs and Instruments

[0064] Chlorogenic acid, prednisone (Hebei Xinyinhe Chemical Co., Ltd.), Shujin Huoxue Tablets (Henan Zhongjie Pharmaceutical Co., Ltd.), type Ⅱ carbonic anhydrase ELISA detection kit, PCR instrument, RNA extraction kit, cDNA Chain synthesis kit, double distilled water, 12-well and 96-well plate, micropla...

Embodiment 3

[0102] Embodiment 3: Prepare freeze-dried powder injection with chlorogenic acid

[0103] 1. Extraction of chlorogenic acid:

[0104] The chlorogenic acid crude drug used in this example is obtained by extracting and purifying Eucommia leaves, with a purity of 99.38%.

[0105] 2. Preparation of chlorogenic acid freeze-dried powder injection

[0106] 2.1 Prescription:

[0107] Chlorogenic acid (main drug) with a purity of 99.38%

[0108] Completely dissolve the above prescription in water for injection, filter, and fine filter with a 0.22 μm sterilizing microporous membrane, adjust the pH, and make a total of 1000 2ml powder injections according to the routine operation of sterile powder injections, each Contains chlorogenic acid 40mg.

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Abstract

The invention provides an application of chlorogenic acid in preparation of medicines for treating osteopetrosis. The chlorogenic acid can be used for effectively treating the osteopetrosis, the treatment effect is better than that of a combined drug of positive drugs such as prednisone and channels-soothing and blood-activating tablets, and the chlorogenic acid is proved to be a safe drug, has small side effects and is good in clinical application prospects.

Description

technical field [0001] The invention relates to the use of chlorogenic acid in the preparation of medicine for treating osteosclerosis. Background technique [0002] Osteosclerosis, also known as marble bone disease, or primary brittle sclerosis, is a rare congenital disease with systemic abnormal bone structure development, and the skull is one of the predilection sites. The bone is extremely dense and loses its original structure, like marble, but the bone becomes more brittle and prone to fractures. It can still be accompanied by anemia, eye atrophy and deafness. It is generally believed that most patients have lesions before birth. According to the clinical manifestations, it is divided into two types: malignant (infant type) and benign (adult type). The former is often stillborn or dies of anemia after birth, and the prognosis is poor. The etiology of osteolithiasis is still unclear, but it may be related to abnormal bone resorption, resulting in excessive deposition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/216A61P19/08
Inventor 张洁张梦甜黄望
Owner SICHUAN JIUZHANG BIO TECH CO LTD
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