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Test strip for detecting pepsinogen I and pepsinogen II as well as detection method and application of test strip

A technology of pepsinogen and test strips, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as prone to errors, and achieve the effects of high precision, accurate ratio results, and small errors

Active Publication Date: 2015-05-13
无锡市江原实业技贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For this reason, the technical problem to be solved by the present invention is that the detection test paper box that is used to detect pepsinogen in the prior art can only detect single pepsinogen, but in calculating the pepsinogen I and II in the sample liquid A technical defect that is prone to errors during the ratio; and then provide a test strip that can simultaneously detect pepsinogen I and II and a test paper card that uses it

Method used

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  • Test strip for detecting pepsinogen I and pepsinogen II as well as detection method and application of test strip
  • Test strip for detecting pepsinogen I and pepsinogen II as well as detection method and application of test strip
  • Test strip for detecting pepsinogen I and pepsinogen II as well as detection method and application of test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Such as figure 1 with 2 As shown, a test strip for detecting pepsinogen I and II of this embodiment includes a bottom plate 1, and absorbent paper 3 and nitrocellulose sequentially adhered to the bottom plate 1 along the length direction of the bottom plate 1. The plain film 2, the binding pad 5, and the sample pad 4; wherein the nitrocellulose film 2 is adhered to the middle part of the bottom plate 1, the absorbent paper 3, the nitrocellulose film 2, and the binding pad 5 and the sample pad 4, which are sequentially in contact with and only with adjacent parts and partially overlap; the nitrocellulose membrane 2 is provided with spaced first sprayed pepsinogen I monoclonal antibody I Detection zone 7, the second detection zone 8 of the pepsinogen II monoclonal antibody II, and the quality control zone 6 sprayed with rabbit anti-mouse polyclonal antibody. The binding pad 5 is sprayed with the surface modified pepsinogen I monoclonal antibody Antibody I and pepsinogen II...

Embodiment 2

[0043] On the basis of Example 1, this example further provides a preparation method of the test strip described in Example 1, including the following steps:

[0044] (1) Antibody pretreatment: select commercial pepsinogen I monoclonal antibody I and pepsinogen II monoclonal antibody II, use 0.05mol / L, pH 7.2-7.6 phosphate buffer at 4℃ Dialysis overnight.

[0045] (2) Preparation of binding pad 5: Add carbodiimide (EDC) (final concentration 20mmol) and pretreated pepsinogen I monoclonal antibody I (microsphere quality: antibody) to the polymer nanosphere solution Mass=50:1), react at room temperature for 2 hours, centrifuge, remove the supernatant, add sample diluent (0.05mol / L containing 1% BSA, pH 7.2 phosphate buffer) to the microsphere concentration of 1.0 mg / ml, to be used. Add carbodiimide (EDC) (final concentration 20mmol) and pretreated pepsinogen II monoclonal antibody I (microsphere mass: antibody mass = 50:1) into the polymer nanosphere solution, and react at room temp...

Embodiment 3

[0049] Such as image 3 As shown, on the basis of Example 1, this example further provides a test paper card for detecting pepsinogen I and II, which includes the test paper strip described in Example 1 and the test strip coated The housing; the housing includes a base and a card cover 9, the card cover 9 is provided with a partial area observation port 11 for exposing the test strip and a sample port 10; wherein the sample port 10 is provided in the Above the sample pad 4 to expose part or all of the sample pad 4 area, the observation port 11 is provided on the upper part of the nitrocellulose membrane 2 to expose the first detection zone 7 and the second detection zone 8 and the quality control belt 6. In this embodiment, the test paper card with a shell is not only convenient for storage and carrying, but also convenient for detection operations during operation; and the setting of the sample addition port 10 is convenient for adding sample liquid during the operation, and t...

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Abstract

The invention relates to a time resolution fluorescence immunochromatographic test strip and test card for simultaneously and quantitatively detecting contents of pepsinogen I and pepsinogen II as well as an application of the test strip. According to the test strip disclosed by the invention, a nitrocellulose membrane is provided with a first detection zone, a second detection zone and a quality control zone which are sprayed at intervals, the first detection zone is coated with a pepsinogen I monoclonal antibody I, the second detection zone is coated with a pepsinogen II monoclonal antibody II, the quality control zone is coated with a rabbit anti-mouse polyclonal antibody, and a bonding pad is coated with a macromolecular nano microspherical coating with the surface decorated by the pepsinogen I monoclonal antibody I and the pepsinogen I monoclonal antibody and containing rare-earth ions; the test strip can simultaneously detect the contents of the pepsinogen I and the pepsinogen II, the problems that a pepsinogen test box cannot simultaneously detect the pepsinogen I and the pepsinogen II in the prior art can be solved, the high accuracy and the small error in the detection result of the pepsinogen I and the pepsinogen II in same sample liquid can be guaranteed, and the great convenience can be provided for the clinical use.

Description

Technical field [0001] The invention relates to a test strip capable of detecting the content of pepsinogen, in particular to a time-resolved fluorescence immunochromatographic test strip and a test paper card capable of simultaneously quantitatively detecting the content of pepsinogen I and II, and an application thereof. In vitro test paper detection technology field. Background technique [0002] Pepsinogen (PG) is the inactive precursor of pepsin in gastric juice. Human pepsin can be divided into two pepsinogen groups with different biochemical and immunological activity characteristics: pepsinogen I (referred to as PG I) and pepsin Original II (referred to as PGII), PGI is mainly secreted by the principal cells of the fundus glands and cervical mucus cells, most of which enter the gastric cavity, and a small amount enters the blood circulation. PGII comes from the whole stomach gland and Brunner's gland of the distal duodenum. PGII synthesized by mucosa is about 25% of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 黄飚张珏周衍钟国良周彬张艺范俊朱岚邓黎莉
Owner 无锡市江原实业技贸有限公司
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