A kind of construction method of small RNA cDNA library

Abstract

The invention discloses a method for constructing a Small RNA cDNA library. A group of DNA molecules disclosed by the present invention, the group of molecules is composed of four molecules as shown in (1)-(4): (1) the DNA molecule shown in SEQ ID No.1; (2) SEQ ID No. The DNA molecule shown in 2; (3) the DNA molecule shown in SEQ ID No.3; (4) the DNA molecule shown in SEQ ID No.4. The method for rapidly separating Small RNA disclosed by the invention solves the problems of long time, complicated operation, high requirements and high toxicity of the current separation of Small RNA by acrylamide gel electrophoresis. Moreover, the method for constructing a Small RNA cDNA library disclosed in the present invention reduces non-specific products and increases the content of target products, and the method is simpler and faster than traditional methods.

Description

technical field [0001] The invention relates to a method for constructing a Small RNA cDNA library. Background technique [0002] Biotechnology and life science will become an important driving force for the new scientific and technological revolution in the 21st century. Many countries, including my country, have listed it as a key research field for priority development. Gene sequencing technology is the core common technology for the development of the biological industry. It has become the commanding heights of science and technology that developed countries are competing to seize, but the related technologies are monopolized by a few European and American companies and the equipment is expensive. [0003] Gene sequencing requires gene sequencers and sequencing reagents, and the production of gene sequencers and kits involves many technologies such as organic chemical synthesis, genetic modification, catalytic enzyme synthesis, and sequencing. The process is complicated a...

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Problems solved by technology

[0002] Biotechnology and life science will become an important driving force for the new scientific and technological revolution in the 21st century. Many countries, including my country, have listed it as a key research field for priority development. Gene sequencing technology, as the core common technology for the development of the biological industry, It has become the commanding heights of science and technology that developed countries are competing to seize, but the related technologies are monopolized by a few European and American companies and the equipment is expensive

[0003] Gene sequencing requires gene sequencers and sequencing reagents, and the production of gene sequencers and kits involves many technologies such as organic chemical synthesis, genetic modification, catalytic enzyme synthesis, and sequencing. The process is complicated and the quality requirements are high. At present, only There are three American manufacturers, and the cost of reagents for one instrument is more than 10 million yuan a year

Due to high consumption, many high-priced sequencers in my country are idle and paralyzed

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