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Application of artemisia verlotorum extract to prevention and control of panax pseudoginseng root rot

A technology of extract and root rot, which is applied in the field of prevention and treatment of root rot of Panax notoginseng by the extract of Artemisia miltiorrhiza, can solve the problems of affecting the growth and quality of notoginseng

Active Publication Date: 2015-05-27
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, diseases in the cultivation process have seriously affected the growth of Panax notoginseng, and the abuse of chemical pesticides has also affected the quality of Panax notoginseng

Method used

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  • Application of artemisia verlotorum extract to prevention and control of panax pseudoginseng root rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 : Preparation of Southern Mugwort Extract

[0019] Select disease-free stems and leaves of Artemisia argyi, clean and dry naturally. The fresh body is crushed through a sieve with a pore size of 50 mesh, and then the stem and leaf powder are mixed and placed in a drying container, and 8 times the amount of ethanol is added to soak at room temperature for 4 days, and then filtered with four layers of gauze to obtain the filtrate. The filter residue was soaked with 6 times the amount of ethanol to repeat the previous step, after repeating twice, the three filtrates were combined. The filtrate is decompressed and concentrated, sterilized by ultraviolet light, and then dilute with sterile water to a crude drug concentration of 2.0g·mL -1 .

Embodiment 2

[0020] Example 2 :The antibacterial effect of southern mugwort extract

[0021] 2.1 Materials and reagents

[0022] South mugwort ethanol extract, sterile water, PDA medium. The tested strain: Fusarium scirpi lamb was provided by the Plant Pathology Laboratory of Agricultural College of Guangxi University.

[0023] 2.2 Method

[0024] The mycelial growth rate method was used to determine the antibacterial effect of the extract of South mugwort. Measure the concentration of crude drug to 2.0g·mL -1 Mugwort extracts of 6.75ml, 3.375ml, 1.6875ml, respectively, with sterile water to make the volume to 10ml, to prepare different concentrations of Mugwort test solutions, respectively take different concentrations of South Mugwort test solution 1ml , Add 14ml of melted PDA medium into a 90mm petri dish to make a drug-containing plate, so that the final test concentration is 0.0900g·mL -1 , 0.0450g·mL -1 And 0.0225g·mL -1 , To add the same amount of sterile water instead of the extract as...

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Abstract

The invention discloses application of an artemisia verlotorum extract to prevention and control of panax pseudoginseng root rot, belonging to the technical fields of plant pest prevention and control. A preparation method of the artemisia verlotorum extract comprises the following steps of (1) naturally airing after cleaning fresh stems and leaves of disease-free artemisia verlotorum, sieving after smashing the fresh stems and leaves of the disease-free artemisia verlotorum, then, mixing the stem powder and the leaf powder, and placing the mixed powder in a dry vessel; (2) adding ethanol into the artemisia verlotorum powder obtained in the step (1) to filter for the first time to obtain a filtrate, adding ethanol into the obtained filter residue again to filter for the second time, repeatedly filtering twice, and then, mixing the filtrates obtained in three times; and (3) carrying out reduced-pressure condensation and ultraviolet sterilization on the filtrate obtained in the step (2), and then, keeping the constant volume by using sterile water to obtain the artemisia verlotorum extract. The artemisia verlotorum extract disclosed by the invention has a favorable antibacterial effect for pathogenic bacteria of panax pseudoginseng root rot.

Description

Technical field [0001] The invention relates to the technical field of disease and insect pest control, and in particular to the use of an extract of southern mugwort to prevent and cure root rot of notoginseng. Background technique [0002] Artemisia verlotorum Lamotte (Artemisia verlotorum Lamotte) belongs to the genus Artemisia of the Compositae family, also known as Artemisia sphagnum and Artemisia spp. Because Artemisia plants are similar in appearance and smell, they can also be collectively referred to as "Mugwort" or "Mugwort". Most of my country, Europe, Asia, Africa, etc. are distributed. This plant is collected perennial, and the plant has a fragrance. The tap root is slightly obvious, with more lateral roots. The stem is solitary or a few, 50-100 cm high, and the branches are 5-8 cm long, obliquely attached to the stem. It can be seen everywhere in the wild and occupies an important position in traditional Chinese culture. It can be used as medicine and has anti-i...

Claims

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Application Information

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IPC IPC(8): A01N65/12A01P3/00
Inventor 黄荣韶向维李良波韦继光甘凤琼张平刚马仲辉
Owner GUANGXI UNIV
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