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Probiotic composition and use thereof for treating picornavirus infection

A microRNA, virus infection technology, applied in the direction of microorganisms, microorganism-based methods, antiviral agents, etc., can solve problems such as no literature reports

Active Publication Date: 2017-10-13
GENMONT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether probiotics can effectively treat the symptoms of enteroviruses has not been reported in the literature.

Method used

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  • Probiotic composition and use thereof for treating picornavirus infection
  • Probiotic composition and use thereof for treating picornavirus infection
  • Probiotic composition and use thereof for treating picornavirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The applicant in this case (Jingyue Biotechnology Company) isolated more than 600 isolates from the gastrointestinal tract samples of healthy adults to establish a strain bank of isolates. The collection status, acquisition time, collectors, and contact information of the collectors are shown in Table 1.

[0075] Table 1. Genetic resource information

[0076]

[0077] Lactobacilli that can inhibit enteroviruses were selected from the strain bank, and the top three Lactobacilli in the analysis results were selected for virus growth inhibition analysis. The preservation numbers, preservation dates and strain names of the three strains of Lactobacillus are shown in Table 2; wherein Lactobacillus paracasei (Lactobacillus paracasei) GMNL-33 and Reuteri Lactobacillus (Lactobacillus reuteri) GMNL-89 are all disclosed above The strain characteristics, preservation certificates, survival test reports and other certification documents and related information have been include...

Embodiment 2

[0081] For the identification of the scientific name of Lactobacillus casei (Lactobacillus casei) GMNL-277, the isolate GMNL-277 was entrusted to Taiwan Hsinchu Food Industry Development Institute to carry out the identification of the bacterial scientific name. Road; the medium is MRS; the culture temperature is 37°C; pathogenicity: none.

[0082] The analysis results show that the isolate GMNL-277 is a Gram-positive bacillus without catalase, oxidase and motility, can grow in both aerobic and anaerobic environments, and will not produce endospores (such as figure 1 shown). In addition, the 16S rDNA partial sequence of the isolate GMNL-277 is shown in SEQ ID No: 1. According to the 16S rDNA analysis, the similarity between the isolate GMNL-277 and Lactobacillus casei is as high as 99%. Then analyzed by the Biolog microbial identification system, the isolate GMNL-277 was the closest to Lactobacillus casei. Therefore, the above results show that the isolate GMNL-277 is Lactob...

Embodiment 3

[0084] The ability of probiotics to inhibit enterovirus type 71:

[0085] In this experiment, three different probiotics were used to test three cell lines, and three different modes were used to test the ability of probiotics to inhibit enterovirus 71, namely pre-treatment, co-incubation and post-treatment. (post-treatment), then use TCID 50 Assay determines the difference in virus load after probiotic treatment, and the concentration of probiotics is at 10 7 CFU / mL, relatively non-cytotoxic to RD, HT-29 and Caco-2 cell lines, with 10 7 The concentration of probiotics in CFU / mL is tested; the antiviral effect is measured at 100TCID 50 As the best, the experimental results are shown in Table 3.

[0086] Table 3. Inhibition percentage of three strains of probiotics inhibiting enterovirus type 71 on three different cell lines (unpaired ttest, *: P<0.05)

[0087]

[0088]The results show that in the RD cells in the pretreatment experiment, the three strains of probiotics o...

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Abstract

The invention discloses a composition for treating picornavirus infection, which comprises Lactobacillus paracasei (GMNL-33) whose preservation number is CCTCC M206133, and Lactobacillus reuteri (GMNL-89) whose preservation number is CCTCC M207154 And Lactobacillus casei (GMNL‑277) preservation number is at least one of CCTCC M2013197, and a pharmaceutically acceptable carrier. Wherein the Lactobacillus casei GMNL‑277 is a novel isolate of Lactobacillus; the present invention also discloses the novel use of the composition or the Lactobacillus for the treatment of picornavirus infection, and its mechanism of action is the combination of the probiotic strain and the virus, and reduce the ability of the virus to infect cells.

Description

technical field [0001] The invention relates to the isolation of novel lactobacillus isolates and the technical field of treating picornavirus infection. Background technique [0002] Enterovirus 71 belongs to the genus Enterovirus of the Family Picornaviridae. The outer sheath of the virus has an icosahedral structure and is not wrapped by a mantle. The acid, alkali and temperature changes in the environment are not easy to affect the activity of the virus. Therefore, it can survive for a long time in the environment and spread widely through fecal-oral transmission. The gene body of the virus is composed of a single-stranded RNA nucleic acid with a size of 7440, which can translate a polyprotein containing 2194 amino acids, including the P1 region of the structural protein, and the P2 and P3 of the non-structural protein area. The polymeric protein is cleaved into P1, P2, and P3 by 2A protease, the P1 region is cleaved into four structural proteins VP1, VP2, VP3, and VP4...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/14A23L33/135C12R1/245
Inventor 王雅芳王贞仁陈奕兴
Owner GENMONT BIOTECH
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