Method for preparing probiotic fresh-cut fruits with vacuum process
A probiotics and vacuum technology, applied in the field of fresh-cut fruit, can solve the problems of bias, cholesterol, and consumer discomfort, and achieve the effect of less investment, increasing varieties and enriching the market.
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Embodiment 1
[0030] Embodiment 1, a kind of method (inoculation Lactobacillus casei in sand pear) utilizing vacuum method to prepare probiotics fresh-cut fruit, carries out following steps successively:
[0031] 1) Lactobacillus casei (L.casei) from Hansen Company was inoculated in 10 mL of pear juice (stock solution), the pH of the pear juice was pre-adjusted to 5.8-6.0 with sodium bicarbonate, and anaerobic dark cultivation was carried out at 37°C for 48 hours. Thereby the activation of Lactobacillus casei (L.casei) is realized. Then, the obtained culture solution was added to the same fruit juice (i.e. the above-mentioned pear juice stock solution adjusted to pH 5.8-6.0), and anaerobic dark culture was carried out at 37°C for about 48 hours until the number of viable bacteria in the obtained probiotic culture solution reached ≥10 8 CFU / ml.
[0032] 2), the sand pear after washing and peeling is cut (for example is the strip of 20 * 4mm), obtains the pear flesh and cuts into pieces.
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Embodiment 2
[0035] Embodiment 2, a kind of method utilizing vacuum method to prepare fresh-cut fruit of probiotics (inoculating lactobacillus plantarum in apple), carries out following steps successively:
[0036] 1) Inoculate domesticated Lactobacillus plantarum (Lactobacillus plantarum) into 10 mL of apple juice (stock solution), adjust the pH of the apple juice to 5.8-6.0 with sodium bicarbonate in advance, and culture it in anaerobic dark at 37°C for 48 hours, so as to achieve plant Activation of Lactobacillus plantarum. Then, the obtained culture solution was added to the same apple juice as above, and anaerobic dark culture was carried out at 37°C for 48 hours until the number of viable bacteria in the obtained probiotic culture solution was greater than or equal to 10 8 CFU / ml.
[0037] 2) Cutting the cleaned and peeled apples (for example into strips of 20×4 mm) to obtain sliced apples.
[0038] 3), first adjust the sugar content of the probiotic culture solution obtained in s...
Embodiment 3
[0041] Embodiment 3, utilize vacuum method to prepare the method for probiotic fresh-cut fruit (inoculate bifidobacterium in apple), carry out following steps successively:
[0042] 1) Inoculate 10mL of apple juice (stock solution) with domesticated Bifidobacterium bifidum (B.bifidum, product number: NJ2301, Jiangsu Yibacteria Biotechnology Co., Ltd.), and the pH of the apple juice was adjusted with bicarbonate in advance. The sodium is adjusted to 5.8-6.0, and anaerobic dark culture is carried out at 37°C for 48 hours. Then, the culture solution is added to the same apple juice as above, and anaerobic dark culture is carried out at 37°C for 48 hours, until the number of viable bacteria in the obtained probiotic culture solution is ≥10 8 CFU / ml.
[0043] 2) Cutting the cleaned and peeled apples (for example into strips of 20×4 mm) to obtain sliced apples.
[0044] 3), first adjust the sugar content of the probiotic culture solution obtained in step 1) to be equal to the su...
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