Method for extracting lycorine from Lycoris plants

Abstract

The invention relates to a method for extracting lycorine from Lycoris plants, which comprises the following steps: 1. extraction: weighing Lycoris plant powder which is subjected to drying, pulverization and screening, adding water with the pH value of 1.5-7.5 according to the material-liquid ratio (g / mL) of 1:(5-25), adding an enzyme which accounts for 1-5 wt% of the Lycoris plant powder, carrying out enzymolysis at 40-80 DEG C for 0.5-2.5 hours, centrifuging for 20 minutes, filtering, and taking the supernate to obtain a lycorine extracting solution; 2. purification: (1) carrying out solvent extraction; (2) adsorbing with a cation exchange resin; (3) carrying out C18 reversed-phase silica gel column chromatography; (4) carrying out normal-phase silica gel column chromatography; and (5) recrystallization: dissolving the dried substance III obtained in the step (4) in ethanol, recrystallizing to obtain a lycorine crude product, filtering, and carrying out vacuum drying at 50-60 DEG C to obtain the lycorine. The method is simple to operate, and has the advantages of low production cost, no environment pollution, short reaction time and higher extraction yield (30.46% higher than other methods). The purification technique has the advantages of environment friendliness and high purity (up to 99.3%).

Description

technical field [0001] The invention relates to a method for extracting lycorine, in particular to a method for extracting lycorine from plants of the genus Lycoris. Background technique [0002] Lycoris radiate.Herb is a perennial herb of the genus Lycoris of the Amaryllidaceae (Amaryllidaceae). It is mainly distributed in Asia. There are 17 species in my country. There are distributions. Amaryllis plants have high ornamental value and are known as "Chinese tulips". Amaryllis bulbs are rich in starch. If you grind them and wash them with plenty of water, eat them after detoxification, the taste is like glutinous rice flour. It is recorded in Materia Medica that it can be fried and soaked in water for overeating. Lycoris is pungent, sweet and warm in nature and has the effects of reducing swelling and killing insects. Its rich alkaloids are also widely used, especially lycorine, which has anti-tumor and anti-viral effects, but content is low. [0003] In the prior art, th...

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Problems solved by technology

[0003] In the prior art, there are mainly methods such as solvent extraction, ultrasonic extraction, microwave extraction, and supercritical fluid extraction, but there are disadvantages such as serious environmental pollution and high production costs.

[0004] Chinese patent 2006100512151 discloses a method for extracting lycorine from plants of the genus Lycoris, and the target object of the extraction is lycorine; the patent focuses on the separation of lycorine, the process is complicated, the operation is cumbersome, and the price of ethyl acetate is relatively high. Requires repeated extraction

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