Ultralow-temperature preservation technique of callus of divaricate saposhnikovia root

A technology of cryopreservation and callus, which is applied in the field of plant tissue culture, can solve the problems that restrict the preservation of windproof germplasm resources, achieve the effect of protecting germplasm resources and reducing the economic cost of preservation

Inactive Publication Date: 2015-06-10
陈桂容
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Problems solved by technology

So far, there have been no reports on the cryopreservation of the callus of the Radix Radi

Method used

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Examples

Experimental program
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Embodiment

[0025] (1) Treatment of explants: Select the full harvested parsnip seeds of the year, gently scrub with detergent water, rinse in tap water for 10 minutes, soak in clean water for 8 hours, and then transfer to 50mg / mL gibberellin Soak in solution for 10h, then soak in 75% ethanol solution for 10s in ultra-clean workbench, rinse with sterile water for 3 times, then disinfect with 0.1% mercury liter solution containing 0.01% Tween-20 for 5min, rinse with sterile water for 3 times Finally, blot the moisture on the surface with sterile filter paper for later use.

[0026] (2) Callus induction: use a sterile scalpel to cut 1 knife on the seeds treated in step (1) to create a wound, then inoculate on the induction medium for callus induction culture, and inoculate at 28°C Culture in total darkness until callus formation. The induction medium is: MS+ 4mg / L 2,4-D+ 1mg / L 6-BA+ 0.5mg / L KT+3.5% sucrose+0.5% agar+0.05% activated carbon, the pH value is 5.8.

[0027] (3) Pre-cultivation...

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PUM

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Abstract

The invention discloses an ultralow-temperature preservation technique of calluses of divaricate saposhnikovia root. A technical divaricate saposhnikovia root in-vitro ultralow-temperature preservation system is established with calluses obtained by inducing divaricate saposhnikovia root seeds as materials, and therefore, the high-quality germplasm resources of the divaricate saposhnikovia root can be effectively protected. According to the technique, the ultralow-temperature preservation of the calluses of the divaricate saposhnikovia root is realized by virtue of the processes of callus induction, pre-culture, loading, dehydration, freezing, washing, re-culture, regeneration and the like; the technique can be taken as a method for preserving the germplasm resources stably for a long time; besides, the economic cost of preservation can be reduced, the valuable and rare germplasm resources can be effectively preserved and great help can be provided for scientific study.

Description

technical field [0001] The invention relates to a method for cultivating plant tissue in agricultural biotechnology, in particular to a technology for cryopreservation of windproof callus. Background technique [0002] windproof ( Saposhnikovia divaricata ) is a medicinal plant of Umbelliferae. Its dried roots are used as medicine. However, due to artificial blindness, excessive excavation, and the deterioration of its habitat, the wild resources of windbreaks are nearly exhausted, and artificial cultivation is mostly used now, and the cultivated varieties are easily affected by environmental conditions, many of which are degraded and of low quality. system, it is very necessary to apply tissue culture technology to the industrial production of windproof, but with the change of callus subculture times and culture conditions, the offspring are prone to variation. In order to avoid callus variation caused by continuous subculture and to establish a new method of germplas...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈桂容
Owner 陈桂容
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