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Allogenic bone supercritical carbon dioxide ungrease treatment method

A carbon dioxide, degreasing treatment technology, applied in medical science, prosthesis and other directions, can solve the problems of cartilage tissue destruction, non-formation and other problems, and achieve the effect of prolonging storage time, safe and free of toxic side effects

Active Publication Date: 2015-06-10
FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technology is aimed at the cartilage of animals, and the cartilage tissue is destroyed after extraction and is not shaped
At present, there is no report of using supercritical carbon dioxide extraction technology to degrease medical bone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for supercritical carbon dioxide degreasing treatment of homogeneous bone, carried out according to the following steps:

[0022] (1) Use human allogeneic bone as raw material, cut into bone segments or bone blocks, or grind into bone powder, remove bone marrow in bone segments or bone blocks with deionized water, until the color of bone tissue is white to yellowish, without bone marrow tissue until;

[0023] The length of the bone segment is 1 cm, and the volume of the bone block is 0.1 cm 3 , the particle size of the bone meal is 100 μm;

[0024] (2) Ultrasonic cleaning of bone segment, bone block or bone powder for 1-8 hours to remove slick oil, and soak for 1 hour in medical alcohol with a mass concentration of 70% after natural cooling;

[0025] (3) Freeze-dry the soaked bone segment, bone block or bone powder, put it into a closed extraction kettle for supercritical carbon dioxide degreasing, the degreasing temperature is 40°C, and the pressure is 41MPa...

Embodiment 2

[0030] A method for supercritical carbon dioxide degreasing treatment of homogeneous bone, carried out according to the following steps:

[0031] (1) Use human allogeneic bone as raw material, cut into bone segments or bone blocks, or grind into bone powder, remove bone marrow in bone segments or bone blocks with deionized water, until the color of bone tissue is white to yellowish, without bone marrow tissue until;

[0032] The length of the bone segment is 10cm, and the volume of the bone block is 3cm 3 , the particle size of the bone powder is 1mm.

[0033] (2) Ultrasonic cleaning of bone segment, bone block or bone powder for 1-8 hours to remove slick oil, and after natural cooling, soak for 2 hours in medical alcohol with a mass concentration of 80%;

[0034] (3) Freeze-dry the soaked bone segment, bone block or bone powder, put it into a closed extraction kettle for supercritical carbon dioxide degreasing, the degreasing temperature is 50°C, and the pressure is 50MPa; ...

Embodiment 3

[0039] A method for supercritical carbon dioxide degreasing treatment of homogeneous bone, carried out according to the following steps:

[0040] (1) Use human allogeneic bone as raw material, cut into bone segments or bone blocks, or grind into bone powder, remove bone marrow in bone segments or bone blocks with deionized water, until the color of bone tissue is white to yellowish, without bone marrow tissue until;

[0041] The length of the bone segment is 5cm, and the volume of the bone block is 1cm 3 , the particle size of the bone meal is 500 μm;

[0042] (2) Ultrasonic cleaning of bone segment, bone block or bone powder for 1-8 hours to remove slick oil, and soaking in medical alcohol with a mass concentration of 75% for 1.5 hours after natural cooling;

[0043] (3) Freeze-dry the soaked bone segment, bone block or bone powder, put it into a closed extraction kettle for supercritical carbon dioxide degreasing, the degreasing temperature is 60°C, and the pressure is 45M...

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Abstract

The invention discloses an allogenic bone supercritical carbon dioxide ungrease treatment method, and belongs to the technical field of medical material treatment. According to the method, a supercritical carbon dioxide extraction technology is adopted, grease in bone segments or bone blocks or bone powder is extracted and removed, and a pretreatment and aftertreatment storage technology of medical bone is further researched through the method. By means of the method, the medical bone is completely ungreased, bone storage time is prolonged, the medical bone has no obvious immunogenicity in use and can be perfectly embedded into original bone tissue of a patient, meanwhile, organic chemical materials harmful to human bodies are not introduced in the preparation process, and the method is safe and free of toxic and side effects.

Description

technical field [0001] The invention belongs to the technical field of medical material processing, and in particular relates to a method for supercritical carbon dioxide degreasing treatment of homogeneous bone. Background technique [0002] Bone materials for medical use have high requirements on bone quality, and it is necessary to keep the bone pollution-free and free of foreign body fat. During the treatment of the same bone material, the lipids in it need to be removed to reduce the immunogenicity of fat and lipoprotein Good for long-term storage. The same bone material is usually degreased by chemical solvent methods such as ether, methanol, and chloroform. The bone is treated with chemical solvents, and there is a small amount of organic solvent residue, which affects the storage time of the bone and the bone regeneration effect. [0003] Carbon dioxide supercritical extraction technology is a mature technology for extracting substances. According to the different ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
Inventor 赵彦涛衷鸿宾朱加亮别晓梅韩丽伟侯树勋
Owner FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
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