Lactobacillus jensenii and pharmaceutical application thereof

A technology of Lactobacillus jennis and bacteria, applied in the biological field, to restore the normal micro-ecological environment of the vagina, increase probiotics in the vagina, and control recurrence

Active Publication Date: 2015-06-10
北京博园新里科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (1) It must be the normal colonizat

Method used

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  • Lactobacillus jensenii and pharmaceutical application thereof
  • Lactobacillus jensenii and pharmaceutical application thereof
  • Lactobacillus jensenii and pharmaceutical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1, Isolation and identification of Lactobacillus jansonii strains

[0049] 1. Isolation of Lactobacillus jansonii strains

[0050] MRS medium: peptone 10.0g; beef extract 10.0g; yeast extract 5.0g; diammonium hydrogen citrate 2.0g; glucose 20.0g; Tween 80, 1.0mL; sodium acetate (CH 3 COONa·3H 2 O) 5.0g; Dipotassium hydrogen phosphate (K 2 HPO4·3H 2 O) 2.0g; Magnesium sulfate (MgSO 4 ·7H 2 O) 0.58g; manganese sulfate (MnSO 4 ·H 2 O) 0.25g; distilled water 1000mL; adjust to pH 6.2-6.6.

[0051] More than 200 samples of vaginal secretions from healthy women without symptoms and signs of vaginitis were collected. The samples were numbered sequentially, inoculated in MRS liquid medium, and incubated anaerobically at 37°C for 24 hours. For each sample, 5 single colonies were randomly selected and cultured in 2 mL MRS liquid medium for 24 hours, and the colonies were numbered sequentially. Bacteria are amplified for bacterial conservation and DNA preparation. ...

Embodiment 2

[0061] Example 2, Functional Analysis of Lactobacillus jensenii IMJ-118

[0062] 1. Lactobacillus jensenii (Lactobacillus jensenii) IMJ-118 produces H 2 o 2 detection

[0063] h 2 o 2 Preparation of standard curve: firstly, piperazine-N, N'-di-ethanesulfonic acid (Piperazine-N, N-Bis 2-ethanesulfonic acid, PIPES) with a concentration of 100mmol / L was used to dissolve 30% (v / v) H 2 o 2 (equivalent to 9.128mol / L) stock solution diluted to 1mol / L, and then 1mol / L H with 100mmol / L PIPES 2 o 2 Dilute to 0μmol / L, 20μmol / L, 40μmol / L, 60μmol / L, 80μmol / L, 100μmol / L working solution; then take 100μL of the above H 2 o 2 Mix the working solution with 100 μL of TMB (tetramethylbenzidine) at a concentration of 20 mmol / L and 2 μL of horseradish peroxidase (1 mg / mL), incubate at 16°C for 10 min, and measure the OD 630 value. end up with H 2 o 2 Concentration standard curve: y=0.0033x-0.0496, R 2 = 0.992.

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Abstract

The invention relates to actobacillus jensenii and application thereof and particularly relates to Lactobacillus jensenii IMJ-118 for preventing and treating bacterial vaginosis. The strain has relatively strong capability of producing H2O2, and can effectively inhibit the growth of external pathogens and other conditional pathogenic bacteria; and meanwhile, the strain has a relatively strong adhesive effect against vaginal epithelial cells and ensures the colonization on vaginal epithelium. Thus, the Lactobacillus jensenii IMJ-118 provided by the invention has certain application prospects in the aspect of preventing and treating bacterial vaginosis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Lactobacillus jensenii IMJ-118 and its use in preparing medicines for preventing and treating bacterial vaginosis. Background technique [0002] Microorganisms inhabit almost every part of the human body, such as the skin, oral cavity, intestinal tract, reproductive tract, etc. There are a large number of bacteria, including many different types of bacteria, which can be roughly divided into "beneficial bacteria" and "conditional pathogenic bacteria". There are two types of germs, and the number and proportion of these bacteria in a healthy human body maintain a stable balance. A variety of bacteria can exist in women's vaginas, among which Lactobacillus is the most common and important beneficial bacteria to maintain a healthy vaginal microecological environment, and Lactobacillus accounts for more than 90% of the total vaginal bacteria in healthy women. The...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P15/02C12R1/225
CPCA61K35/747C12N1/205C12R2001/225
Inventor 张素华雷天箫陈晏李博宇
Owner 北京博园新里科技有限公司
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