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Anti polycyclic aromatic hydrocarbon monoclonal antibody, hybridoma cell PYR#6-E4 generating same, and applications thereof

A monoclonal antibody, hybridoma cell technology, applied in applications, microorganisms, biochemical equipment and methods, etc., can solve problems such as being unsuitable for large-scale sample analysis and monitoring, expensive instruments and equipment, and time-consuming, and achieve easy preparation and scale. The effect of chemical production

Inactive Publication Date: 2015-06-17
许丽 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole process is cumbersome, time-consuming, expensive instruments and equipment, not suitable for the analysis and monitoring of a large number of samples in a short time, and organic solvents such as methanol, dichloromethane, acetone, etc. are needed in this process

Method used

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  • Anti polycyclic aromatic hydrocarbon monoclonal antibody, hybridoma cell PYR#6-E4 generating same, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0027] Experiment 1 The preservation number is CGMCC No.8552 hybridoma cell line and its secreted monoclonal antibody preparation

[0028] Four BALB / C mice were immunized with self-made immunogen PBA-BSA, and negative serum was collected from the orbit before immunization. The immunization method is as follows: take 60 μg of immunogen, dilute it to 200 μl with normal saline, add an equal volume of Freund’s adjuvant, and mix the solution and adjuvant with a mixing device to form water-in-oil. The well-mixed immunogen was injected subcutaneously for 6-8 points. A booster immunization was carried out every two weeks, and the amount of immunization was 30 μg protein / mouse. After the fourth booster immunization, the titer was detected by ELISA method, and two mice with higher titer were selected, and the immunization dose was 50 μg protein / mouse, and intraperitoneal impact immunization once.

[0029] One week after the last immunization, mouse splenocytes and SP2 / 0 cells were tak...

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PUM

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Abstract

The invention discloses an anti polycyclic aromatic hydrocarbon monoclonal antibody hybridoma cell strain PYR#6-E4 and a monoclonal antibody generated by the same. The preservation number of the provided anti polycyclic aromatic hydrocarbon monoclonal antibody hybridoma cell strain is CGMCC No. 8552. The hybridoma cell strain can stably secrete anti polycyclic aromatic hydrocarbon monoclonal antibody, which has a high titer and can be applied to development of polycyclic aromatic hydrocarbon ELISA detection kit.

Description

technical field [0001] The invention relates to an anti-polycyclic aromatic hydrocarbon monoclonal antibody, a hybridoma cell producing the antibody and its application, especially the application in the detection kit of polycyclic aromatic hydrocarbons. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a large class of organic pollutants widely present in the environment, consisting of two or more aromatic rings. It is mainly produced by the incomplete combustion of organic matter such as coal and petroleum. Many types of PAHs and their metabolites can enter the human body through respiratory and skin contact, and have a strong carcinogenic effect on the human body. [0003] The monitoring and analysis technology of polycyclic aromatic hydrocarbons mainly includes the following aspects: [0004] 1. Due to the pollution caused by PAHs to the environment and the threat to human health, some countries have stipulated ...

Claims

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Application Information

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IPC IPC(8): C12N5/20C12N15/06C07K16/44G01N33/577
Inventor 许丽赵鲁程言君
Owner 许丽