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Non-pathogenic F18 E. coli strain and use thereof

An Escherichia coli, non-pathogenic technology, applied in the direction of bacteria, blood diseases, antibacterial drugs, etc., can solve the problems of low reliability and efficacy

Active Publication Date: 2015-06-24
伊兰科加拿大有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] A related drawback of many conventional therapeutic or prophylactic compositions and methods for intestinal bacterial infections associated with symptoms of edematous disease and / or diarrheal disease is their low reliability and efficacy

Method used

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  • Non-pathogenic F18 E. coli strain and use thereof
  • Non-pathogenic F18 E. coli strain and use thereof
  • Non-pathogenic F18 E. coli strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] a) Non-pathogenic F18 E. coli strain

[0032] For Escherichia coli (EcL), Dr. J.M. Fairbrother isolated large intestine from pig feces in 1996 at the OIE reference laboratory (Faculté de medicine vétérinaire (FMV), Université de Montréal (UdeM), Saint-Hyacin, Quebec, Canada) Bacillus strain, said Escherichia coli strain was deposited in the International Depository Authority of Canada (IDAC) on June 20, 2013 and has an accession number of 200613-01.

[0033] Identification of the strains was performed using the API system. The API 20E code for Master Seed is 5004552. The serotype of the strain is shown as O141:K94:H-serotype.

[0034] The virulence type of the strains was determined by colony hybridization and / or polymerase chain reaction (PCR). Virulence type results show that the strain is positive for F18 and AIDA, but negative for the following toxins: STa, STb, LT, EAST1, Stx1, Stx2, CNF, F4, F5, F6, F17, F41, P, AFA, Eae , Paa, Tsh, and aerogenin genes. There...

Embodiment 2

[0039] In one broad aspect, the purpose of the study was to evaluate the effect of different oral doses of the non-pathogenic F18 strains described herein on their fecal excretion and anti-F18 systemic humoral response.

[0040] Table 1 below generally outlines the research schedule:

[0041]

[0042]

[0043] I-Study Design

[0044] a) animals

[0045] Blood samples from 50 lactating piglets were collected for F18 receptor status analysis, and the piglets' ears were marked. Selected weaner pigs (male and female) aged between 17 and 20 days were purchased from a local farm in Montérégie, Quebec, Canada. Transport animals to an isolation facility. Selected farms were free of recent (less than 6 months) Porcine Reproductive and Respiratory Syndrome (PRRS) and post-weaning disease events. Pigs were healthy pigs and weighed between 4Kg and 6Kg. A total of twenty-one (21) pigs positive for F18 receptor status were used and randomized using ear tag numbers into three (3) ...

Embodiment 3

[0102] In one broad aspect, the purpose of the study was to evaluate the efficacy of a composition comprising a combination of the non-pathogenic F4 and F18 E. coli strains described herein.

[0103] Table 4 below generally outlines the research schedule:

[0104] number of days

event

0

Pigs arrive at the experimental unit and are divided into 2 groups

0 to 5

Adaptation period

1, 12, 14, 16, 18 and 19

with stool sampling for PCR

4, 12, 17 and 19

blood sample

5

Oral dose or placebo

12, 13 and 14

to attack

12, 14, 16, 18 and 19

Fecal sampling for viable counts of challenge strains

19

Final autopsy and end of experiment

[0105] I-Study Design

[0106] a) animals

[0107] Blood samples from 50 lactating piglets were collected for F18 receptor detection and analysis, and the piglets' ears were marked. Selected weaner pigs (male and female) aged between 17 and 21 days wer...

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Abstract

There is provided an isolated E. coli strain deposited at the International Depositary Authority of Canada (IDAC) on Jun. 20, 2013 and attributed accession number 200613-01. There is also provided methods of using this strain for preventing edema disease or diarrhea caused by an F18 pathogenic E. coli infection in an animal.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application 61 / 674,179, filed July 20, 2012, the contents of which are hereby incorporated by reference in their entirety. technical field [0003] This application relates to non-pathogenic F18 Escherichia coli (E. Coli) strains and uses thereof. Background technique [0004] Edema and diarrhea are often common ailments in animals kept by breeders. For example, edema disease in weaned piglets is usually caused by Shiga toxin-encoding Escherichia coli (STEC) strains encoding Shiga toxin type 2e (Stx2e) (MacLeod et al., 1991, Vet Pathol 28:66-73), whereas secretory diarrhea in newborn and weaned piglets is usually caused by enterotoxigenic Escherichia coli strains encoding heat stable (STa, STb, EAST1) and / or heat labile (LT) enterotoxins. erichia coli strain, ETEC) (Gyles and Fairbrother, 2010, Escherichia coli. In: Pathogenesis of bacterial infections in animals,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P1/12A61P31/04C12R1/19A23K10/18A23K20/00A23K50/30
CPCA61K35/74C12R2001/19C12N1/205A61P1/04A61P1/12A61P31/04A61P7/10Y02A50/30C12N1/20
Inventor 约翰·莫里斯·费尔布勒特埃里克·纳多克拉里斯·德索泰尔
Owner 伊兰科加拿大有限公司
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