Bacillus subtilis and application of bacillus subtilis in simple and elegant liquor
A Bacillus subtilis, light and elegant technology, applied in the field of microbial liquor fermentation, to shorten the fermentation cycle and improve the typical flavor
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Strain Isolation
[0025] Take Daqu for brewing from Hubei Chuyuanchun Wine Industry Co., Ltd., treat it in an 80°C water bath for 15 minutes to kill non-spore bacteria, spread it on a plate after appropriate dilution, and pick typical single colonies according to the colony shape (the colony is nearly round, 3 -4.5mm, dull, central protuberance, blunt-toothed edges, off-white), Bacillus was roughly screened out by microscopic examination.
[0026] The formula of LB medium (1L): tryptone 10g, yeast extract 5g, NaCl 10g; LA plus agar 15g.
[0027] Re-screening and identification
[0028] Through physiological and biochemical and 16SrDNA sequence identification, a strain of Bacillus subtilis, Bacillus subtilis (Bacillus subtilis) CYC18 was finally screened out. This bacterium was sent to the China Center for Type Culture Collection on January 16, 2015 for preservation. The taxonomic designation: Bacillus subtilis CYC18 Bacillus subtilis CYC18; deposit number: CCTCC NO.M...
Embodiment 2
[0031] Scale-up culture of Bacillus subtilis CYC18
[0032] According to glucose 20g / L, yeast extract 10g / L, peptone 10g / L, NaCl 5g / L, KH 2 PO 43g / L, prepare fermentation broth with 65% (V / V) liquid content, sterilize by high-temperature steam at 121°C for 30min, cool down to 30°C, inoculate 1% (v / v ), cultivated at 30°C, and controlled the stirring speed to maintain dissolved oxygen (DO value 30%-90%). Fermented for 24 hours and placed in tanks, the plate count of Bacillus subtilis CYC18 was 4.8×10 9 cfu / ml.
Embodiment 4
[0034] Enzyme activity detection of CYC18 strain
[0035] Prepare starch, casein and cellulose agar plates (the above three media are commercially available Qingdao Haibo commercial medium), recover the CYC18 strain in a PA bottle, take 5 μl and spot on the plate, and cultivate. Observe whether there are hydrolysis rings on the casein and cellulose plates, add iodine solution dropwise around the starch plate inoculated with bacteria, observe whether there are hydrolysis rings, and measure the diameter of the hydrolysis rings.
[0036] Table 1 Degradation ability of CYC18 strain
[0037] Degradable plate
PUM

Abstract
Description
Claims
Application Information

- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com