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A kind of monoclonal antibody hybridoma 3a6 of toxoplasma gondii protein tgvp1 and monoclonal antibody

A monoclonal antibody and hybridoma technology, applied in the field of cell engineering, can solve problems such as unclear mechanism of action, and achieve high specificity and sensitivity

Active Publication Date: 2018-03-09
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TgVP1 contains 17 transmembrane domains, and the N-terminal has a signal peptide sequence, which can guide TgVP1 into the secretory pathway of Toxoplasma gondii, but the specific mechanism of action is still unclear

Method used

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  • A kind of monoclonal antibody hybridoma 3a6 of toxoplasma gondii protein tgvp1 and monoclonal antibody
  • A kind of monoclonal antibody hybridoma 3a6 of toxoplasma gondii protein tgvp1 and monoclonal antibody
  • A kind of monoclonal antibody hybridoma 3a6 of toxoplasma gondii protein tgvp1 and monoclonal antibody

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Embodiment 1

[0015] Example 1 Preparation and identification of the monoclonal antibody of the present invention

[0016] 1. Preparation of monoclonal antibody 3A6

[0017] 1) Design, preparation and carrier coupling of Toxoplasma gondii TgVP1 antigen polypeptide

[0018] According to the Toxoplasma gondii TgVP1 sequence on GenBank (accession number: EPT25031.1), the Toxoplasma gondii TgVP1 protein sequence contains 816 amino acids. TMHMM software was used to analyze the transmembrane domain of TgVP1 protein, and IEDB software was used to analyze the immunogenicity, hydrophilicity and hydrophobicity and surface accessibility of TgVP1 protein. And strong. After the above analysis, the final screening polypeptide sequence is: 292aa-320aa, SEQ ID NO:2. The short peptide (TgVP1-P1) and the short peptide (TgVP1-P1-KLH) whose C-terminus is coupled to keyhole limpet hemocyanin carrier protein KLH were synthesized by Shanghai Gil Biochemical Company.

[0019] 2) Immune mice

[0020] 6-8 week ...

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Abstract

The invention discloses a monoclonal antibody hybridoma 3A6 of toxoplasma gondii protein TgVP1 and the monoclonal antibody. The Toxoplasma gondii protein TgVP1 monoclonal antibody hybridoma 3A6 of the present invention was deposited in the China Center for Type Culture Collection on December 16, 2014, and the preservation number is CCTCC NO: C2014231. In the present invention, mice are immunized with a synthetic peptide of Toxoplasma protein TgVP1, and a hybridoma cell line 3A6 resistant to Toxoplasma protein TgVP1 is obtained through hybridoma technology. The monoclonal antibody secreted by it can specifically recognize TgVP1 protein, and can be used in TgVP1 experiments The identification of protein has application value.

Description

technical field [0001] The invention relates to the field of cell engineering, in particular to the hybridoma cell line 3A6 and the monoclonal antibody of the toxoplasma protein TgVP1 monoclonal antibody. Background technique [0002] V-H+-PPase is a unique proton pump that exists in many animals and plants. It can hydrolyze the phosphate anhydride bond of inorganic pyrophosphate, and use the released energy to transport protons, generate an electrochemical gradient across the membrane, and play a role similar to the proton pump ATPase. V-H+-Ppase can efficiently convert stored energy into H+ and / or transfer membrane gradients for a variety of different intracellular transport processes. V-PPases were first found in plants and photosynthetic bacteria, and recent studies have shown that trypanosoma, Leishmania, Toxoplasma gondii, and Plasmodium falciparum also have this enzyme. It is worth noting that this enzyme does not exist in the animal hosts of these parasites, so V-P...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/40G01N33/577G01N33/573
Inventor 罗树红郝文波肖斌
Owner SOUTHERN MEDICAL UNIVERSITY
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