Chinese caterpillar fungus barley

A technology for barley and Cordyceps militaris, applied in the field of bioengineering, can solve problems such as affecting the significant efficacy of Cordyceps sinensis, and achieve the effects of being easy to realize large-scale production, wide application prospects, and broad application development prospects

Inactive Publication Date: 2015-07-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interestingly, cordycepin is mainly present in Cordyceps militaris, and its content in Cordyceps sinensis is very small, but the absence of cordycepin did not affect the significant efficacy of Cordyceps sinensis

Method used

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  • Chinese caterpillar fungus barley

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The barley was processed by the DS56-Ⅲ twin-screw extruder produced by Jinan Juxian Machinery Equipment Co., Ltd. The barley used was commercially available, and its moisture content was 14.5% as determined by a moisture analyzer. The feed rate was set at 120 kg / h. Table 1 lists the temperature combination and expansion degree of the three sections. It can be seen from Table 1 that when the temperatures in the preheating zone, transportation zone, and ripening zone are 60, 160, and 190°C respectively, the puffing degree of barley is the highest. After further drying, the expansion degree at different water contents was investigated. It can be seen from Table 2 that the puffing effect is the best when the moisture content is about 14-18%, so commercially available barley can be directly used for puffing.

[0019] Table 1: The puffing degree of puffed barley obtained by the combination of different temperatures in different sections of the extrusion puffing machine

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Embodiment 2

[0024] Inoculate the Cordyceps sinensis strain into a 250ml Erlenmeyer flask with 50ml medium, the composition of the medium is: glucose 20g / l, potato juice 200g / l, natural pH, 25°C, shaker speed 150 rpm, culture for 96 hours , as seeds for inoculation.

[0025] Inoculate the Cordyceps militaris strain into a 250ml Erlenmeyer flask with 50ml of culture medium. The culture medium is composed of: glucose 20g / l, potato juice 200g / l, natural pH, 25°C, shaker speed 150 rpm, and culture for 96 hours as seeds for inoculation.

[0026] Take puffed barley with a puffing degree of 2.5 and barley with a mechanical crushing degree of 1mm, mix a total of 50g, add 40g of distilled water, put it into a 500ml Erlenmeyer flask, sterilize at 121°C for 20min, and inoculate each of the seed liquid of Cordyceps sinensis and Cordyceps militaris after cooling. 5ml, in a constant temperature and humidity incubator to control the ambient humidity to 90%, the temperature is 25°C, and measure the conte...

Embodiment 3

[0030] Mix 30g of puffed barley with a puffing degree of 2.5 and 20g of barley with a mechanical crushing degree of 2mm, add 40g of distilled water, put it into a 500ml Erlenmeyer flask, sterilize at 121°C for 20min, and inoculate the cultivated Cordyceps sinensis and Cordyceps militaris seeds after cooling Each 5ml of solution is controlled environment humidity 80% in constant temperature and humidity incubator, and temperature is 15 ℃, and after 20 days of fermentation, it is 5.3% to measure cordycepin content.

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Abstract

The invention relates to Chinese caterpillar fungus barley, which takes barley as a medium, and employs Cordyceps sinensis and cordyceps militaris for performing dixenie mixed solid state fermentation, which belongs to the technical field of biology engineering. According to the invention, barley is obtained through dixenie biotransformation, and has health preserving and health caring efficacy and good application prospect.

Description

technical field [0001] The method adopts Ophiocordyceps sinensis and Cordyceps militaris and uses barley as a culture medium to carry out double-bacteria mixed solid-state fermentation, which belongs to the technical field of bioengineering. The preservation number of the bacterial strain Cordyceps militaris is CICC 14013, and the preservation number of the bacterial strain Ophiocordyceps sinensis is CICC 14088, and the nutrition and health barley is obtained through double-bacteria mixed solid-state fermentation. Background technique [0002] Cordyceps belongs to Fungi, Ascomycota, Ascomycetes, Sordariomycetidae, Hypocreales, Clavcipitaceae . Most of the fungi of the genus Cordyceps are medicinal fungi, among which Ophiocordyceps sinensis is generally known and has the longest application history, and Cordyceps militaris has the most in-depth application research. [0003] According to literature research, Cordyceps sinensis was first recorded in "Yuewang Yaozheng" in 710...

Claims

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Application Information

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IPC IPC(8): A23L1/18A23L1/29A01G1/04C05F11/00A23L33/00
Inventor 蔡宇杰王小梅白玉管政兵廖祥儒张大兵
Owner JIANGNAN UNIV
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