Chinese caterpillar fungus barley
A technology for barley and Cordyceps militaris, applied in the field of bioengineering, can solve problems such as affecting the significant efficacy of Cordyceps sinensis, and achieve the effects of being easy to realize large-scale production, wide application prospects, and broad application development prospects
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Embodiment 1
[0018] The barley was processed by the DS56-Ⅲ twin-screw extruder produced by Jinan Juxian Machinery Equipment Co., Ltd. The barley used was commercially available, and its moisture content was 14.5% as determined by a moisture analyzer. The feed rate was set at 120 kg / h. Table 1 lists the temperature combination and expansion degree of the three sections. It can be seen from Table 1 that when the temperatures in the preheating zone, transportation zone, and ripening zone are 60, 160, and 190°C respectively, the puffing degree of barley is the highest. After further drying, the expansion degree at different water contents was investigated. It can be seen from Table 2 that the puffing effect is the best when the moisture content is about 14-18%, so commercially available barley can be directly used for puffing.
[0019] Table 1: The puffing degree of puffed barley obtained by the combination of different temperatures in different sections of the extrusion puffing machine
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Embodiment 2
[0024] Inoculate the Cordyceps sinensis strain into a 250ml Erlenmeyer flask with 50ml medium, the composition of the medium is: glucose 20g / l, potato juice 200g / l, natural pH, 25°C, shaker speed 150 rpm, culture for 96 hours , as seeds for inoculation.
[0025] Inoculate the Cordyceps militaris strain into a 250ml Erlenmeyer flask with 50ml of culture medium. The culture medium is composed of: glucose 20g / l, potato juice 200g / l, natural pH, 25°C, shaker speed 150 rpm, and culture for 96 hours as seeds for inoculation.
[0026] Take puffed barley with a puffing degree of 2.5 and barley with a mechanical crushing degree of 1mm, mix a total of 50g, add 40g of distilled water, put it into a 500ml Erlenmeyer flask, sterilize at 121°C for 20min, and inoculate each of the seed liquid of Cordyceps sinensis and Cordyceps militaris after cooling. 5ml, in a constant temperature and humidity incubator to control the ambient humidity to 90%, the temperature is 25°C, and measure the conte...
Embodiment 3
[0030] Mix 30g of puffed barley with a puffing degree of 2.5 and 20g of barley with a mechanical crushing degree of 2mm, add 40g of distilled water, put it into a 500ml Erlenmeyer flask, sterilize at 121°C for 20min, and inoculate the cultivated Cordyceps sinensis and Cordyceps militaris seeds after cooling Each 5ml of solution is controlled environment humidity 80% in constant temperature and humidity incubator, and temperature is 15 ℃, and after 20 days of fermentation, it is 5.3% to measure cordycepin content.
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