Preparation method of temperature-sensitive hydrogel based on functionalized graphene oxide and application thereof

A temperature-sensitive hydrogel, functionalized technology, applied in the field of medicine, to achieve the effects of abundant raw materials, increased concentration and good effect

Inactive Publication Date: 2015-07-08
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, a natural polymer compound-chitosan is grafted on the surface of functionalized graphene oxide, and prepared into a thermosensitive hydrogel, and it is used as a functionalized hyperthermia medium and drug delivery carrier in the preparation of photothermal targeted therapy for tumor treatment. Drug application has not yet been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of preparation method based on functionalized graphene oxide temperature-sensitive hydrogel of the present invention, in specific implementation, is realized by the following steps:

[0019] 1) Weigh 80 mg of graphene oxide and 400-600 mg of chitosan and dissolve them in 40 ml of 2-(N-morpholine) ethanesulfonic acid buffer, and ultrasonically obtain a uniformly dispersed suspension for 1 h. Add 480 mg of methylaminopropyl)-3-ethylcarbodiimide hydrochloride and 640 mg of N-hydroxysuccinimide to the suspension in turn, and continue to sonicate for 6 hours to obtain a reaction solution; magnetically stir the reaction solution at room temperature for 16 hours , then filtered through a 0.2 μm water membrane, pumped and washed with ultrapure water until neutral to remove unreacted chitosan, resuspended the resulting filter cake with 16ml of ultrapure water, pre-frozen at -20°C, and freeze-dried for 48 hours That is, functionalized graphene oxide (GO-CS);

[0020] 2) W...

Embodiment 2

[0024] 1) Weigh 100 mg of graphene oxide and 500 mg of chitosan and dissolve them in 50 ml of 2-(N-morpholine) ethanesulfonic acid buffer, ultrasonicate for 1 h to obtain a uniformly dispersed suspension, weigh 1-(3-dimethyl Add 600mg of aminopropyl)-3-ethylcarbodiimide hydrochloride and 800mg of N-hydroxysuccinimide to the above suspension in turn, continue ultrasonication for 6h, and then magnetically stir at room temperature for 16h to form a mixed solution. The mixture was filtered through a 0.2 μm water membrane, and washed with ultrapure water to neutrality to remove unreacted chitosan, and the obtained filter cake was resuspended with 20ml of ultrapure water, pre-frozen in a -20°C refrigerator, and frozen Dry for 48 hours to obtain functionalized graphene oxide (GO-CS);

[0025]2) Weigh 5 mg of functionalized graphene oxide and dissolve it in 5 ml of ultra-pure water, probe for 40 times at a power of 400w, and probe once every 5s, each time for 5s, to obtain a functiona...

Embodiment 3

[0029] 1) Weigh 120 mg of graphene oxide and 600 mg of chitosan and dissolve them in 60 ml of 2-(N-morpholine) ethanesulfonic acid buffer, ultrasonicate for 1 h to obtain a uniformly dispersed suspension, weigh 1-(3-dimethyl Add 720 mg of aminopropyl)-3-ethylcarbodiimide hydrochloride and 960 mg of N-hydroxysuccinimide to the above suspension in sequence, and continue to sonicate for 6 hours to obtain a reaction solution; magnetically stir the reaction solution at room temperature for 16 hours , then filtered through a 0.2 μm water membrane, pumped and washed with ultrapure water until neutral to remove unreacted chitosan, resuspended the obtained filter cake with 24ml ultrapure water, pre-frozen at -20°C, and freeze-dried for 48 hours , to obtain functionalized graphene oxide;

[0030] 2) Weigh 6mg of functionalized graphene oxide and dissolve it in 6ml of ultrapure water, and probe for 40 times at a power of 400w, once every 5s, each time for 5s, to obtain a functionalized g...

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Abstract

The invention relates to a preparation method of temperature-sensitive hydrogel based on functionalized graphene oxide and an application thereof and effectively solves a problem of the preparation of the functionalized graphene oxide temperature-sensitive hydrogel and the application of the functionalized graphene oxide temperature-sensitive hydrogel as a thermal-therapy medium and a drug transport carrier in preparation of a tumor treatment photo-thermal targeted drug. In the method, the temperature-sensitive hydrogel based on the functionalized graphene oxide is prepared by mixing the functionalized graphene oxide on which a drug is carried with poloxamer P407 / P188 gel, wherein the volume ratio of the functionalized graphene oxide on which the anti-tumor drug is carried to the poloxamer P407 / P188 gel is 1-3:1. Water-soluble macromolecular chitosan being strong in reaction activity is connected to the surface of the original graphene oxide to form a water-soluble functionalized carrier, wherein the functionalized carrier is mixed with chitosan temperature-sensitive hydrogel to obtain the temperature-sensitive hydrogel based on the functionalized graphene oxide. The raw materials are wide in sources. The temperature-sensitive hydrogel is abundant in raw materials, is low in cost, is good in effect, can increase the concentration of the carried drug at the position of tumors, and is a great creative achievement on tumor treatment drugs.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of a thermosensitive hydrogel based on functionalized graphene oxide. Background technique [0002] In recent years, malignant tumors have seriously threatened human life and health, and their treatment has always been the focus of human attention. With the continuous emergence of new anti-tumor drugs, chemotherapy has shown unique advantages and broad prospects for development. However, antitumor drugs do not selectively act on tumor cells, but simultaneously act on normal cells, especially vigorously growing cells such as bone marrow and epithelial tissue. As an intelligent temperature-sensitive drug delivery system, poloxamer in situ gel has low toxicity, low irritation, and good biocompatibility; it can improve drug bioavailability and therapeutic effect, and can reduce drug side effects. [0003] Thermosensitive hydrogel is a kind of smart hydro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/06A61K47/04A61K47/34A61K47/36A61K45/00A61K31/337A61P35/00
Inventor 祝侠丽张英杰黄河清侯琳张慧娟张振中
Owner ZHENGZHOU UNIV
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