Major gene TaTGW-2A for controlling thousand-grain weight of triticum aestivum and CAPS marking method of major gene TaTGW-2A
A technology of major gene and marker method, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve the problem of unclear molecular regulation mechanism of grain weight
Inactive Publication Date: 2015-07-15
ANHUI AGRICULTURAL UNIVERSITY
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However, the molecular regulation mechanism of grain weight is still unclear
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[0038] The present invention will be described in further detail below according to the drawings and embodiments.
[0039] (1) Wheat Genomic DNA Extraction
[0040] The test materials used for DNA extraction were parental Jing 411 and Hongmangchun 21, two high-low mixed pools, recombinant inbred line RIL (Jing 411 / Hongmangchun 21) F 9 There are 262 germplasm resource materials in each family of the group. The specific method is:
[0041] 1. Grind the wheat single grain, take 0.1g and add 0.7mL SDS extract (0.1M Tris-HCl (PH=8.5), 0.1M NaCl, 0.05M EDTA (PH=8.0), 2% SDS) at 60°C Under cracking for 45min, during which several times of shock.
[0042] 2. Centrifuge at 12000 rpm at 4°C for 10 minutes.
[0043] 3. Take the supernatant and add an equal volume of phenol: chloroform: isoamyl alcohol (25:24:1) and rotate up and down until the two phases do not separate quickly.
[0044] 4. Centrifuge for 10 minutes at 12000 rpm at 4°C.
[0045] 5. Take the supernatant and add an e...
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The invention discloses a major gene TaTGW-2A for controlling the thousand-grain weight of triticum aestivum and further discloses a CAPS marking method of the major gene TaTGW-2A for controlling the thousand-grain weight of triticum aestivum. The major gene TaTGW-2A for controlling the thousand-grain weight of triticum aestivum and the CAPS marking method of the major gene TaTGW-2A have the beneficial effects that a candidate region which is closely associated with the grain weight of triticum aestivum is assayed by using a simplified genome sequencing technology, a candidate gene of the region is cloned, proved by the combined verification of artificial populations and natural populations, the gene is a novel major gene which has remarkable influence on grain weight, and a CAPS marker (TaTGW-2A) which is easier to detect compared with an SNP (Single Nucleotide Polymorphism) marker is developed. According to the major gene TaTGW-2A for controlling the thousand-grain weight of triticum aestivum and the CAPS marking method of the major gene TaTGW-2A, a novel gene resource is provided for the high-yield breeding of triticum aestivum, and meanwhile, a molecular mechanism of yield formation is further explained.
Description
technical field [0001] The invention relates to a major gene TaTGW-2A for controlling wheat thousand-grain weight and a CAPS marking method thereof, belonging to the technical field of crop genetics and breeding. Background technique [0002] Wheat (Triticum aestivum L.) is the second largest food crop in the world. Increasing wheat production is of great strategic significance to national food security and agricultural production benefits. Thousand-grain weight is one of the three elements of wheat yield and has high heritability (Giura and Saulescu, 1996). Therefore, the breeding process of wheat is to continuously increase grain weight to a large extent. However, the molecular regulation mechanism of grain weight is still unclear. [0003] Summarizing the previous reports, thousand-grain weight is a quantitative trait, which is jointly controlled by major and minor effect polygenes. At present, there are many QTL loci related to grain weight, which are widely distribute...
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IPC IPC(8): C12N15/29C12Q1/68
Inventor 胡明建常成张海萍卢杰朱晓峰刘鹏马刚邵辉司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY