Method for detecting phytic acid based on carbon dot fluorescence off-on mode

A phytate and carbon dot fluorescence technology, applied in the analysis and detection of phytate, based on the carbon dot fluorescence off-on mode detection of phytate field, can solve the problem that liquid chromatography equipment is expensive, operators Need professional training, poor selectivity and sensitivity, etc., to shorten the sample detection time, improve the sample detection efficiency, and easy to operate.

Active Publication Date: 2015-07-15
TIANJIN UNIV
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Problems solved by technology

Precipitation and colorimetric methods are more intuitive, but have poor selectivity and sensitivity
Liquid chromatography has the disadvantages of e

Method used

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  • Method for detecting phytic acid based on carbon dot fluorescence off-on mode
  • Method for detecting phytic acid based on carbon dot fluorescence off-on mode

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Experimental program
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Effect test

Embodiment 1

[0026] Taking phytate contained in corn seeds as the detection object, the specific operation steps are as follows:

[0027] (1) Preparation of carbon dot fluorescent probe: 4.2 g of citric acid and 2.1 g of lysine were added to 40 mL of ultrapure water. After stirring for 2 to 3 minutes, add it to the reaction kettle. The mixed solution was reacted at 200° C. for 5 hours. After the reaction, the solution was dialyzed in pure aqueous solution for 36 hours to remove impurities, and the carbon dots obtained by dialyzing were diluted to 0.2 mg / L with 10 mmol / L (pH=5.7) morpholineethanesulfonic acid buffer;

[0028] (2) Prepare phytic acid standard solution: use 10mmol / L (pH=5) morpholine ethanesulfonic acid buffer to prepare standard sample stock solution of phytic acid, the stock solution concentration is 0.1mmol / L to 10mmol / L L, the stock solution is configured into standard solutions of different concentrations with morpholinoethanesulfonic acid buffer solution;

[0029] (3...

Embodiment 2

[0034] The specific operation steps are as follows:

[0035] (1) Preparation of carbon dot fluorescent probes: 4.2 g of citric acid and 1.4 g of lysine were added to 40 mL of ultrapure water. After stirring for 2 to 3 minutes, add it to the reaction kettle. The mixed solution was reacted at 200° C. for 5 hours. After the reaction, the solution was dialyzed in pure aqueous solution for 36 hours to remove impurities, and the carbon dots obtained by dialyzing were diluted to 0.1 mg / L with 10 mmol / L (pH=5.7) morpholineethanesulfonic acid buffer;

[0036](2) Prepare phytic acid standard solution: use 10mmol / L (pH=6) morpholine ethanesulfonic acid buffer to prepare standard sample stock solution of phytic acid, the stock solution concentration is 0.1mmol / L to 10mmol / L L, the stock solution is configured into standard solutions of different concentrations with morpholinoethanesulfonic acid buffer solution;

[0037] (3) Fluorescence quenching: add 20μL 10mM FeCl to 2mL 0.1mg / L carb...

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Abstract

The invention relates to a method for detecting phytic acid based on carbon dot fluorescence off-on mode. The carbon dot fluorescence is quenched with ferric ion, then, a phytic acid sample is added, the fluorescence is recovered, with increase of concentration of a to-be-detected sample, the recovered fluorescence varies from weak to strong, and qualitative and quantitative detection is realized. The method comprises the steps of preparing a carbon dot fluorescence probe; configuring phytic acid standard solutions of a series of concentration; determining fluorescence intensity before and after reaction between middle carbon dot/ferric ion and phytic acid, building a standard curve according to the relation between the phytic acid concentration and the relative fluorescence intensity variation value; detecting variation of fluorescence intensity before and after reaction between the to-be-detected sample and the carbon dot/Fe(III), calculating the relative fluorescence intensity variation value so as to obtain the content of phytic acid in the to-be-detected sample. According to the fluorescence analysis method established by the invention, phytic acid can be quickly and effectively detected, the sample detection efficiency can be improved, the sample detection time can be shortened, and the operation is simple and rapid.

Description

technical field [0001] The invention relates to a method for analyzing and detecting phytic acid, in particular to a method for detecting phytic acid based on a carbon dot fluorescence off-on mode, belonging to the technical field of fluorescence analysis and detection. Background technique [0002] Phytic acid, also known as phytic acid, is widely found in plant foods such as grains and beans. Phytate is an important organophosphorus additive, widely used in food, medicine, chemical industry, daily chemical, metallurgy and other industries due to its unique structure, chemical properties, physiological and pharmacological functions. It is generally considered that phytic acid is an anti-nutritional agent. However, many studies in recent years have shown that it has many physiological activities. Therefore, phytic acid has attracted more and more attention. Quantitative analysis of phytic acid is particularly important to deeply understand its physiological role and provide...

Claims

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Application Information

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IPC IPC(8): G01N21/76
CPCG01N21/6428G01N2021/6432
Inventor 苏荣欣高召黄仁亮齐崴
Owner TIANJIN UNIV
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