Application of flagellar hook protein FlgE of reorganized pseudomonas aeruginosa

A technology of flagellar hook protein and Pseudomonas aeruginosa, which can be applied in the direction of medical preparations containing active ingredients, antibacterial drugs, drug combinations, etc., and can solve problems affecting the extension and function of flagellar body

Inactive Publication Date: 2015-07-22
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As for the flagellar hook, only a few studies have shown that if its component molecule FlgE is mutated, it will affect the elongation and function of the flagellar body

Method used

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  • Application of flagellar hook protein FlgE of reorganized pseudomonas aeruginosa
  • Application of flagellar hook protein FlgE of reorganized pseudomonas aeruginosa
  • Application of flagellar hook protein FlgE of reorganized pseudomonas aeruginosa

Examples

Experimental program
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Embodiment Construction

[0025] Recombinant expression and purification of FlgE (small-scale preparation)

[0026] According to the FlgE coding gene sequence of Pseudomonas aeruginosa, the upstream and downstream primers for amplifying the full-length FlgE coding sequence with NdeI and HindIII restriction sites were designed and synthesized. The above primers were used to amplify the extracted Pseudomonas aeruginosa genomic DNA. The PCR program was: denaturation at 94°C for 45 seconds, annealing at 58°C for 1 minute, extension at 72°C for 1 minute, and 25 cycles; After separation and purification by agarose gel electrophoresis, it was digested with NdeI and HindIII and recovered, and ligated with the expression vector PET24a that had undergone the same digestion; the ligated product was transformed into E. 50 μg / mL) on LB agar plate, select Kan-resistant clones after overnight culture, extract the plasmid after shaking the bacteria for amplification, and carry out enzyme digestion identification. Afte...

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Abstract

The invention belongs to the field of biological immunity, and disclosesapplication of flagellar hook protein FlgE of reorganized pseudomonas aeruginosa. The flagellar hook protein FlgE of the reorganized pseudomonas aeruginosa is used for preparing an immunological adjuvant or a vaccine, in particular to be used as the immunological adjuvant in preventing or treating tumors or infectious diseases. The invention provides an FlgE monomer which uses a pseudomonas aeruginosa PAO1 strain as a model bacterial strain and proves recombinant expression, so that corneal epithelial cells of people or lung tissues of mice can be stimulated to generate an inflammatory reaction, remarkable adjuvant effects can be shown, and a foundationis laidfor applying the FlgE monomer to the immunological adjuvant.

Description

technical field [0001] The invention belongs to the field of biological immunity and relates to the use of recombinant Pseudomonas aeruginosa flagellin hook protein FlgE. Background technique [0002] Although there are differences in the immunological structure of humans, livestock, and poultry, there are many commonalities in the biological model of the immune response to antigens. The immune adjuvants commonly used in the field of human disease prevention and control also play a role in poultry and livestock. . As a whole, the improvement of the health quality and the extension of the average life expectancy of human beings largely benefit from the resistance to various pathogens that can cause epidemics; the latter depends on the development of vaccines against various pathogens and the development of immunization methods Establish. At present, in some areas, pathogen infection is still a major killer that threatens human health, such as HIV infection that causes AIDS,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/00A61P35/00A61P37/04A61P31/04A61P31/00A61P33/00
CPCY02A40/70
Inventor 王宜强沈莹唐华邵联波
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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