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A medium for increasing the content of β-glucan in space-mutated yeast strains and its application

A technology of space mutagenesis and culture medium, applied in the direction of fungi, microorganism-based methods, microorganisms, etc., can solve the problems of increased content of β-glucan, low content of extracted polysaccharides, single fermentation method, etc., achieving low cost, The effect of high bioavailability and reduction of production cost

Active Publication Date: 2017-09-22
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a medium for increasing the content of β-glucan in yeast space mutagenesis strains, so that the content of β-glucan can be significantly increased, so as to overcome the shortcomings of the existing single fermentation method and low content of extracted polysaccharides.

Method used

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  • A medium for increasing the content of β-glucan in space-mutated yeast strains and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: culture medium and culture method

[0034] (1) Fermentation medium: 3.45g of glucose, 3.60g of corn steep liquor, 2.80g of yeast extract, 0.45g of glycerin, make up to 100mL with distilled water.

[0035] (2) Activation: Pick 2-3 rings of the Saccharomyces cerevisiae mutant strain FFLM2.0016 strain preserved on the slant and inoculate them in a conical flask containing 50 ml of the medium prepared in step (1), and culture at 28°C with shaking at 200r / min 12h;

[0036] (3) After the bacterial classification is activated by step (2), insert it into a 50L fermenter with an inoculum size of 4% (v / v), fill it with the medium prepared in step (1), and culture it with shaking at 26°C and 450r / min 72h, that is.

Embodiment 2

[0037] Embodiment 2: culture medium and culture method

[0038] (1) Fermentation medium: 3.70g of glucose, 3.95g of corn steep liquor, 3.10g of yeast extract, 0.70g of glycerol, make up to 100mL with distilled water, set aside.

[0039] (2) Activation: Pick 2-3 rings of the Saccharomyces cerevisiae mutant strain FFLM2.0016 strain preserved on the slant and inoculate them into a conical flask containing 50 ml of the medium prepared in step (1), and culture with shaking at 26°C and 200r / min 12h;

[0040] (3) After the bacterial classification is activated by step (3), insert it into a 50L fermenter with an inoculum size of 4% (v / v), fill it with the medium prepared in step (1), and culture it with shaking at 28°C and 450r / min 72h.

Embodiment 3

[0041] Embodiment 3: culture medium and culture method

[0042] (1) Fermentation medium: glucose 3.56g, corn steep liquor 3.76g, yeast extract 2.98g, glycerin 0.53g, make up to 100mL with distilled water, set aside.

[0043] (2) Activation: Pick 2-3 loops of the Saccharomyces cerevisiae mutant strain FFLM2.0016 strain preserved on the slant and inoculate them into a conical flask containing 50 ml of the medium prepared in step (1), and culture at 27°C with shaking at 200r / min 12h;

[0044] (3) After the bacterial classification is activated by step (3), insert it into a 50L fermenter with an inoculum size of 4% (v / v), and fill it with the culture medium prepared in step (1), and culture it with shaking at 27°C and 450r / min 72h.

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Abstract

The invention relates to a culture medium for increasing content of beta-glucan of space mutation yeast strains and an application of the culture medium. The culture medium comprises components of raw materials as follows: 3.45-3.70 g of glucose, 3.60-3.95 g of corn steep liquor, 2.80-3.10 g of a yeast extract and 0.45-0.70 g of glycerin, and the culture medium is complemented by water to 100 mL; culture conditions are as follows: a 50 L fermentation tank is inoculated with the strains with the inoculation quantity of 4%(v / v), and the culture medium is contained in the fermentation tank, and the strains are cultured in a shaking manner at the speed of 450 r / min at the temperature of 26-28 DEG C for 72 h. According to the culture medium and a culture method, Saccharomyces cerevisiae cell walls are taken as raw materials, a carbon source, a nitrogen source, inorganic ions and an enzyme activator in the culture medium are optimized through single factor testing and response surface analysis, Saccharomyces cerevisiae mutant strains FFLM2.0016 are cultured in the fermentation tank, and the content of the beta-glucan can be obviously increased.

Description

technical field [0001] The invention relates to a culture medium and a culture method for increasing the content of β-glucan in a yeast space mutagenesis strain. Background technique [0002] The cell wall of Saccharomyces cerevisiae accounts for about 20% to 30% of the dry weight of the cell, and the yeast cell wall is composed of about 29% β-glucan, 31% mannan, 13% protein, and 9% lipid And 1 ~ 2% chitin composition. The main physiological function of β-glucan and chitin is to maintain the structure of the cell wall and maintain the normal physiological shape of the cell. [0003] β-glucan is a polysaccharide of cell wall structure, located in the innermost layer of the cell wall, connected with the protoplast membrane, mainly used as a scaffold to protect the outer mannan, and is the main component of the yeast cell wall. Yeast β-glucan has significant physiological effects. Studies have found that it can bind to specific macrophage receptors to enhance the body's immun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12R1/865
CPCC12N1/16
Inventor 刘红芝王强刘丽石爱民胡晖巩阿娜李亚楠段玉权林伟静
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI