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Thalassospira sp. capable of degrading polycyclic aromatic hydrocarbons under saline environment and application thereof

A polycyclic aromatic hydrocarbon and spirulina technology, which is applied in the field of environmental pollution biological treatment, can solve the problems of low tolerance concentration, narrow microbial salt tolerance range, and cannot be applied to high-cyclic aromatic hydrocarbon pollution biological treatment, etc. The effect of applying value

Active Publication Date: 2015-07-22
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It can be seen that the range of salt tolerance of these microorganisms is relatively narrow, and the tolerance concentration is low
Most salt-tolerant microorganisms only have high degradation performance for low-ring aromatic hydrocarbons, and cannot be applied to the biological treatment of high-ring aromatic hydrocarbon pollution in high-salt environments

Method used

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  • Thalassospira sp. capable of degrading polycyclic aromatic hydrocarbons under saline environment and application thereof
  • Thalassospira sp. capable of degrading polycyclic aromatic hydrocarbons under saline environment and application thereof
  • Thalassospira sp. capable of degrading polycyclic aromatic hydrocarbons under saline environment and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Isolation and identification of Thalassospira sp.TSL5-1 (Thalassospira sp.TSL5-1, CGMCC No.10278)

[0031] The long-term oil-contaminated soil from Shengli Oilfield was used as the screening soil. The specific screening scheme is as follows:

[0032] 1) Take 5.0g of soil, add it to MSM medium (mineral salt of pyrene (20mg / L) as the only carbon source and energy source) with a salinity of 5.0%, culture at 30°C and 170rpm constant temperature shaking; after acclimatization for 15 days, take The cultured bacteria solution was transferred to fresh medium with successively increasing pyrene concentration with 10% inoculum amount, and was subcultured under the same culture conditions. This was done continuously for 5 times.

[0033] Wherein the composition of the MSM medium with a salinity of 5.0% is: 3.0g / L Na 2 SO 4 , 0.3g / L NH 4 Cl, 0.15g / L CaCl 2 2H 2 O, 0.2g / L KH 2 PO 4 , 0.5g / L KCl, 50g / L NaCl, 7.5g / L MgCl 2 ·6H 2 O, 1.0ml / L trace element solution, ...

Embodiment 2

[0071] Embodiment 2, Thalassospira sp.TSL5-1 (Thalassospira sp.TSL5-1, CGMCC No.10278) performance of degrading pyrene

[0072] 1) Medium containing pyrene (pyrene is the only carbon source and energy source): dissolve pyrene in acetone, filter and sterilize, and then add it to the MSM medium with a salinity of 5.0%, so that the final concentration of pyrene in the medium is The concentration is 20mg / L;

[0073] 2) Bacterial inoculum: pick the Helicotrium TSL5-1CGMCC No.10278 obtained in Example 1 and inoculate it in a medium containing pyrene, and culture it with shaking for 7 days. Take the culture solution and centrifuge at 5000rpm for 10min, remove the supernatant, centrifuge and wash three times, and resuspend in fresh MSM medium to make the OD of the bacterial solution 600 It is about 0.25, and it is used as the inoculum solution of the degradation experiment.

[0074] 3) Strain growth and pyrene degradation experiments: the above-mentioned inoculum solution was inocul...

Embodiment 3

[0077] Example 3, Thalassospira sp.TSL5-1 (Thalassospira sp.TSL5-1, CGMCC No.10278) degradation performance of pyrene at different salinities

[0078] 1) Medium containing pyrene at different salinities (pyrene is the only carbon source and energy source): dissolve pyrene in acetone, filter and sterilize, and then add to the medium with a salinity of 0.5%, 1.5%, 3.5%, and 5% , 9.5% and 19.5% MSM medium (the preparation of each salinity medium refers to the MSM medium of 5.0% salinity, and the addition content of NaCl is adjusted accordingly according to the salinity requirements), so that the final content of pyrene in the medium The concentration is 20mg / L;

[0079] 2) Bacterial inoculum: pick the Helicotrium TSL5-1CGMCC No.10278 obtained in Example 1 and inoculate it in a medium containing pyrene, and culture it with shaking for 7 days. Take the culture solution and centrifuge at 5000rpm for 10min, remove the supernatant, centrifuge and wash three times, and resuspend in fr...

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Abstract

The invention discloses thalassospira sp. capable of degrading polycyclic aromatic hydrocarbons under saline environment and application thereof. The preservation number of the provided thalassospira sp. TSL5-1 is CGMCC No. 10278. The invention further provides application of the thalassospira sp. or bacterial suspension thereof or culture product thereof or fermentation product thereof in polycyclic aromatic hydrocarbon degradation. Based on experiments, compared with the prior art, the thalassospira sp. TSL5-1 (CGMCC No. 10278) can grow and propagate under the saline environment by taking the polycyclic aromatic hydrocarbons, such as phenanthrene, pyrene, fluoranthene, benzopyrene and benzanthracene, as the unique carbon source and energy source.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollution, and in particular relates to a sea spiny strain capable of degrading organic pollutants of polycyclic aromatic hydrocarbons in a salt environment and an application thereof. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of organic compounds composed of two or more benzene rings arranged in different ways such as linear, angular or clustered. PAHs pollutants are widely distributed in environmental media such as air, water, soil and its sediments. PAHs in the environment mainly come from natural activities (such as forest fires, decay of animal and plant remains, formation of oil and coal, volcanic eruptions, etc.) and human activities (such as wood processing, petroleum smelting, transportation, gas manufacturing, military facilities, dangerous disposal and landfill, etc.). Most of the p...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/20
CPCA62D3/02A62D2101/20C12N1/205C12R2001/01
Inventor 王慧周海燕黄勇梁承月方婷婷
Owner TSINGHUA UNIV
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