Circulating tumor DNA EGFR detecting technology and reagent kit thereof

A detection technology and kit technology, applied in the field of circulating tumor DNA EGFR detection technology and its kits, can solve the problems of high experimental operation requirements, expensive instruments, complicated operations, etc., and achieve improved sensitivity, fast detection speed, and simple operation. Effect
CN104789677AInactive Publication Date: 2015-07-22张道允 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
张道允
Publication Date
2015-07-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a circulating tumor DNA EGFR (Epidermal Growth Factor Receptor) detecting technology and a reagent kit thereof. The circulating tumor DNA EGFR detecting technology comprises the following steps: step 1, designing a synthetic primer, a closed probe and a detecting probe for a mutation site; step 2, processing a sample and extracting DNA; step 3, applying the circulating tumor DNA EGFR detecting technology to the reagent kit, and establishing a PCR amplified reaction system. According to the circulating tumor DNA EGFR detecting technology disclosed by the invention, the specific MGB Blocker probe, the EGFR specific probe and the primer are adopted to detect the EGFR mutation in the circulating tumor DNA; the MGB Blocker probe is utilized to retard nonspecific amplification of the EGFR wild type genome and improves the sensitivity; the circulating tumor DNA EGFR detecting technology can be used for detecting the EGFR mutation in the circulating tumor DNA, is simple to operate, cheap in detection, capable of being popularized at a large scale and high in detection speed; the detecting process can be completed for about 2 hours.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a circulating tumor DNA EGFR detection technology and a kit thereof. Background technique

[0002] Epidermal growth factor receptor (EGFR) is a member of the human epidermal growth factor receptor (HER) family, belongs to the receptor tyrosine kinase family, and is the expression product of the proto-oncogene c-erbB1. EGFR is mainly located in the cell membrane, and after being activated by ligands such as EGF, dimerization occurs and triggers phosphorylation of the intracellular segment, generating tyrosine kinase activity and further activating downstream signaling pathways (Pao W et al.Proc Natl Acad Sci US A.2004 ; 101:13306-11), regulating cell growth, proliferation and other physiological processes. EGFR downstream signaling pathways mainly include: Ras / Raf / MEK / ERK / MAPK pathway, PI3K / PDK1 / Akt pathway, PLC-γ pathway, JAK / STAT pathway, etc. (Sun J M et al.Lung Cancer.2010; 68:42...

Claims

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