Method for producing gluconic acid through bioconversion of lignocellulose

A technology of lignocellulose and gluconic acid, which is applied in the field of bio-manufacturing and bio-refining of bio-based chemicals, can solve the problems of limited number of catalyst cycles, high catalyst consumption, increased production cost of gluconic acid products, etc. Separation and purification, low matrix cost, low cost effect

Active Publication Date: 2015-07-29
CATHAY R&D CENT CO LTD +1
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  • Abstract
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Problems solved by technology

However, there are many technical difficulties in the production of gluconic acid by fermentation of lignocellulose as a substrate. At present, most of the methods used in the reports on the production of gluconic acid by using lignocellulose raw materials are heterogeneous catalytic oxidation methods.
In the process of producing gluconic acid by these methods, a large amount of chemicals such as sulfuric acid and sodium hydroxide need to be used, and the catalyst is mostly Na 2 Cu(OH) 4 And gold / phosphotungstic heteropolyacid cesium salt, although this method has a high conversion rate, the catalyst used is limited in the number of cycles, and the catalyst consumption per unit product is high, which increases the production cost of gluconic acid products in disguise

Method used

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  • Method for producing gluconic acid through bioconversion of lignocellulose

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Effect test

Embodiment 1

[0035] Mix the dried corn stalks with 5% (w / w) dilute sulfuric acid solution at a mass ratio of 2:1, pretreat for 3 minutes at 190°C and 1.2MPa; adjust the pH of the material after pretreatment To 5.5, insert the cultured biological detoxification strain Amorphotheca resinae ZN1 (CGMCC7452), and cultivate it statically for 7d under the condition of 25°C; then add the detoxified material into the reactor, and adjust the solid content to 25% ( w / w), adding cellulase (amount of 15FPU / g DM), saccharifying at 50°C and 150rpm for 48h, and controlling the pH at 4.8 with 5M sodium hydroxide during the saccharifying process. The concentration of glucose in the obtained corn stalk hydrolyzate was 71.42g / L. The corn stalk hydrolyzate was centrifuged at 10,000 rpm for 10 min and used as a culture medium, and was inserted into the Aspergillus niger (Aspergillus niger M276, purchased from Shanghai Institute of Industrial Microbiology Strain Collection Center) seed culture medium with 10% in...

Embodiment 2

[0037] Mix the dried corn stalks with 5% (w / w) dilute sulfuric acid solution evenly at a mass ratio of 2:1, pretreat for 3 minutes at 190°C and 1.2MPa; add the pretreated materials directly Into the reactor, adjust the solid content to 25% (w / w), add cellulase (amount of 15FPU / gDM), saccharify at 50°C and 150rpm for 48h, use 5M sodium hydroxide to control pH during saccharification In 4.8. The concentration of glucose in the obtained corn stalk hydrolyzate was 75.36g / L. The corn stalk hydrolyzate was centrifuged at 10,000rpm for 10min and used as a culture medium, and was inserted into the seed culture medium of Aspergillus niger (Aspergillus niger M276, purchased from Shanghai Institute of Industrial Microbiology Strain Collection Center) with 10% inoculum, and fermented in a liquid state at 30°C for 168h. The rotating speed is 200rpm, the concentration of gluconic acid in the fermentation broth is 79.04g / L, and the conversion rate to glucose is 95%.

[0038] The cellulosic...

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Abstract

The present invention relates to a method for efficiently producing gluconic acid through bioconversion of lignocellulose by using Aspergillus niger. The method specifically comprises: inoculating cultured Aspergillus niger in a lignocellulose hydrolyzate to ferment so as to produce gluconic acid, wherein the gluconic acid concentration can achieve 80 g / L after fermentation for 96 h; or simultaneously adding Aspergillus niger being subjected to liquid culture, a lignocelluloses raw material, and cellulase into a reactor, and carrying out synchronous saccharification and fermentation so as to produce gluconic acid. According to the present invention, the method has characteristics of short time and high efficiency, the lignocellulose has the wide source and the low price, and the broad development space and the broad development prospects are provided.

Description

【Technical field】 [0001] The invention relates to the field of bio-manufacturing and bio-refining of bio-based chemicals, in particular to a method for producing high-concentration gluconic acid through saccharification and fermentation of lignocellulosic raw materials such as corn stalks. 【Background technique】 [0002] Gluconic acid and its salts have the characteristics of non-toxicity, good thermal stability and non-deliquescence, and are widely used in chemical industry, food, medicine, light industry and other industries. Especially in the aspect of cement admixture, sodium gluconate can be widely used as a water reducer and retarder, and has achieved good results; in the food industry, it can be used as a nutritional supplement, curing agent, buffer, etc., and now people Sodium gluconate has been used as a raw material to develop a new seasoning with high taste. In addition, due to its excellent chelating properties, gluconic acid is widely used in various industrial...

Claims

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Application Information

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IPC IPC(8): C12P7/58
CPCC12P7/58C12P2203/00
Inventor 鲍杰张宏森张建
Owner CATHAY R&D CENT CO LTD
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