Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A highly cross-linked uniform particle size agarose gel medium prepared by ultrasonic inverse suspension method

A reverse suspension method, agarose gel technology, applied in chemical instruments and methods, other chemical processes, alkali metal compounds, etc., to achieve the effect of improving recovery rate, reducing production cost, and controllable parameters

Active Publication Date: 2017-09-29
海家福(茂名)食品有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention discloses a highly cross-linked agarose gel medium with uniform particle size prepared by an ultrasonic inverse suspension method to solve the problems of gel particle uniformity and mechanical properties, in order to improve the quality of the agarose gel medium and reduce its production cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] Specific embodiments of the present invention will be further described in detail below.

[0022] The present invention prepares a method for highly cross-linked uniform particle size agarose gel medium using an ultrasonic inverse suspension method, the steps of which are:

[0023] a. Accurately measure 2-20 volume ratio of oily liquid and accurately measure 1 volume ratio of organic solvent and mix them in a ratio of 2-20:1 to make an oil phase solution;

[0024] b. Adding a nonionic surfactant to the oil phase solution to adjust the hydrophilic-lipophilic balance value of the oil phase solution to between 3-20;

[0025] c. Ultrasonicate the above system with 100w-1000w for 5-30 minutes, mix well and preheat to 60-90°C;

[0026] d. Accurately weigh agarose with a weight ratio of 0.4 to 4, and sodium chloride with a weight ratio of 0.18 to a volume ratio of 20 with ultrapure water, heat and melt to make 2% to 20% of the water phase Agarose solution;

[0027] e. Heat ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing highly cross-linked agarose gel medium with uniform particle size by using the ultrasonic inverse suspension method. The method of the highly cross-linked agarose gel medium with uniform particle size, the present invention firstly prepares the oil phase solution through ultrasonic mixing and then prepares 2% to 20% agarose solution, then mixes and emulsifies the solution prepared on it again, and passes The gel medium of highly cross-linked uniform particle size agarose was prepared by sieve suction filtration and reaction and preserved in 20-80% ethanol. The invention is mainly used in pharmaceutical manufacturers.

Description

technical field [0001] The invention relates to a preparation process of an agarose gel medium, in particular to a highly cross-linked agarose gel medium with uniform particle diameter prepared by an ultrasonic inverse suspension method. Background technique [0002] The application of agarose gel matrix in the biomedical industry can be traced back to the 1960s and 1970s. Due to the neutrality of agarose, it can be well compatible with biological macromolecules without specific binding, and is easy to be modified and crossed. The combination and other characteristics make it quickly become the most widely used medium in the field of biomedicine. After nearly half a century of application, agarose gel has been greatly developed in terms of mechanical strength, porosity, biocompatibility, chemical stability and derivatization ability. However, the production technology and market of agarose gel medium are still monopolized by foreign companies. The sales price of agarose ge...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30
Inventor 陈江磊潘恩山潘奔前那妹
Owner 海家福(茂名)食品有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com