Preparation method of bacterial cellulose with antimicrobial property

A technology of bacterial cellulose and cellulose, applied in the field of microorganisms, can solve problems such as complicated processes, and achieve the effect of simplifying the complex preparation process and solving toxic effects.

Active Publication Date: 2015-08-19
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation process goes through multiple steps and the process is complicated
And at present, there is no report o

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Preparation of seed liquid: Take two rings of bacteria from the slant of Gluconacetobacter sp. GD-BC-1CCTCC M 208149 and put them into a 500mL Erlenmeyer flask containing 50mL of liquid activation medium, at 28°C, 100r / m, with a stroke of 65 mm and vibrated for 36 hours to obtain seed liquid. (The formula components per liter of liquid activation medium are as follows: 20g of glucose, 5g of peptone, 5g of yeast extract, 2.7g of disodium hydrogen phosphate, 1.15g of citric acid, and the balance is water. After mixing the above ingredients evenly, adjust the pH value 6.0, sterilized for use).

[0020] (2) Fermentation of bacterial cellulose: Take 5mL of seed liquid and add it to 500mL Erlenmeyer flask with 50mL of fermentation medium, 28 ℃ reciprocating shaker shake culture for 36 hours, shaker speed is 60r / m, stroke is 75mm, get fermentation broth. (The fermentation medium contains 20g of glucose per liter, 5g of yeast extract, 10g of peptone, 1g of citric acid, 1...

Embodiment 2

[0026] (1) Preparation of seed liquid: Take two rings of bacteria from the slant of Gluconacetobacter sp. GD-BC-1CCTCC M 208149 and put them into a 500mL Erlenmeyer flask containing 50mL of liquid activation medium, at 28°C, 100r / m, with a stroke of 65 mm and vibrated for 24 hours to obtain seed liquid. (The formula components per liter of liquid activation medium are as follows: 20g of glucose, 5g of peptone, 5g of yeast extract, 2.7g of disodium hydrogen phosphate, 1.15g of citric acid, and the balance is water. After mixing the above ingredients evenly, adjust the pH value 6.0, sterilized for use).

[0027] (2) Fermentation of bacterial cellulose: Take 5 mL of seed liquid and add to 80 mL of fermentation medium in a 500 mL Erlenmeyer flask, shake and culture on a rotary shaker at 28° C. for 18 hours at a speed of 20 r / m to obtain a fermentation liquid. (The fermentation medium contains 40g of glucose per liter, 1g of yeast extract, 8g of peptone, 8g of disodium hydrogen p...

Embodiment 3

[0033] (1) Preparation of seed liquid: Take two rings of bacteria from the inclined plane of Gluconacetobacter sp. GD-BC-1CCTCC M 208149 and put them into a 500mL Erlenmeyer flask containing 50mL of liquid activation medium, at 32°C, 80r / m, with a stroke of 75 mm and vibrated for 18 hours to obtain seed liquid. (The formula components per liter of liquid activation medium are as follows: 50g of glucose, 10g of peptone, 8g of yeast extract, 1.5g of disodium hydrogen phosphate, 1g of citric acid, and the balance is water. The above-mentioned ingredients are mixed evenly, and the pH value is adjusted to 6.0. for sterilization).

[0034] (2) Fermentation of bacterial cellulose: Take 3mL of seed liquid and add it to 60mL of fermentation medium in a 500mL Erlenmeyer flask, shake and cultivate in a reciprocating shaker at 32°C for 18 hours, the shaker speed is 20r / m, and the stroke is 65mm, to obtain fermentation broth. (The fermentation medium contains 60g of glucose per liter, 0...

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Abstract

The invention discloses a preparation method of bacterial cellulose with the antimicrobial property. Gluconacetobacter GD-BC-1 is taken for fermentation cultivation, to obtain the bacterial cellulose contained fermentation liquor, antimicrobial peptide produced bacteria are added into the fermentation liquor, the antimicrobial peptide produced bacteria are embedded in the bacterial cellulose to continue to ferment, the cellulose group contained bacteria liquid is obtained, a cellulose group is taken out to be placed into an MRS (Methicillin Resistant Staphylococcus) culture medium to be cultured, the cellulose group is taken out to be rinsed through water, and the bacterial cellulose with the antimicrobial property is obtained. According to the preparation method of the bacterial cellulose with the antimicrobial property, the antimicrobial peptide produced bacteria are wrapped in the bacterial cellulose synthesis process by a mixed multi-fermentation method; the fermentation culture medium is transformed to enable antimicrobial peptide to be synthesized through the antimicrobial peptide produced bacteria to be hung on the bacterial cellulose and accordingly the bacterial cellulose with the antimicrobial property is prepared; the problem of the toxic effect on the bacterial cellulose produced bacteria due to direct adding of an antibacterial agent is solved; the complicated preparation process of the traditional antimicrobial bacterial cellulose is simplified.

Description

Technical field: [0001] The invention belongs to the field of microorganisms, and in particular relates to a preparation method of bacterial cellulose with antibacterial properties. Background technique: [0002] Bacterial cellulose is a polymer secreted by bacteria. In addition to the characteristics of general cellulose, it also has many unique properties. It has a three-dimensional network structure. Bacterial cellulose has a high ability to bind water; the surface area is larger than that of plant fibers, which has a higher chemical adsorption capacity; better mechanical properties, which have high elasticity, high resilience and high tensile strength. These many advantages make bacterial cellulose a potential as a dressing for wounds. [0003] The ingredient of bacterial cellulose is only cellulose, which has no antibacterial function. In order to make bacterial cellulose have antibacterial function, soaking method and chemical grafting method are currently used. T...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/04C12P21/00C12N11/12C12N11/04C12R1/02C12R1/46C12R1/245C12R1/01
Inventor 冯劲施庆珊谢小保黄小茉
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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