Preparation method of bacterial cellulose with antimicrobial property

Abstract

The invention discloses a preparation method of bacterial cellulose with the antimicrobial property. Gluconacetobacter GD-BC-1 is taken for fermentation cultivation, to obtain the bacterial cellulose contained fermentation liquor, antimicrobial peptide produced bacteria are added into the fermentation liquor, the antimicrobial peptide produced bacteria are embedded in the bacterial cellulose to continue to ferment, the cellulose group contained bacteria liquid is obtained, a cellulose group is taken out to be placed into an MRS (Methicillin Resistant Staphylococcus) culture medium to be cultured, the cellulose group is taken out to be rinsed through water, and the bacterial cellulose with the antimicrobial property is obtained. According to the preparation method of the bacterial cellulose with the antimicrobial property, the antimicrobial peptide produced bacteria are wrapped in the bacterial cellulose synthesis process by a mixed multi-fermentation method; the fermentation culture medium is transformed to enable antimicrobial peptide to be synthesized through the antimicrobial peptide produced bacteria to be hung on the bacterial cellulose and accordingly the bacterial cellulose with the antimicrobial property is prepared; the problem of the toxic effect on the bacterial cellulose produced bacteria due to direct adding of an antibacterial agent is solved; the complicated preparation process of the traditional antimicrobial bacterial cellulose is simplified.

Description

Technical field: [0001] The invention belongs to the field of microorganisms, and in particular relates to a preparation method of bacterial cellulose with antibacterial properties. Background technique: [0002] Bacterial cellulose is a polymer secreted by bacteria. In addition to the characteristics of general cellulose, it also has many unique properties. It has a three-dimensional network structure. Bacterial cellulose has a high ability to bind water; the surface area is larger than that of plant fibers, which has a higher chemical adsorption capacity; better mechanical properties, which have high elasticity, high resilience and high tensile strength. These many advantages make bacterial cellulose a potential as a dressing for wounds. [0003] The ingredient of bacterial cellulose is only cellulose, which has no antibacterial function. In order to make bacterial cellulose have antibacterial function, soaking method and chemical grafting method are currently used. T...

AI Technical Summary

Problems solved by technology

The preparation process goes through multiple steps and the process is complicated

And at present, there is no report on the preparation of bacterial cellulose with antibacterial properties by mixed multiple fermentation methods in China.