Method for detecting ionic calcium content of blood sample

A technology of ionized calcium and blood samples, which is applied in the field of calcium ion detection, can solve the problems of poor stability and specificity, and achieve the effects of good stability, simple measurement steps and high measurement accuracy

Inactive Publication Date: 2015-08-19
李宏奎
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Problems solved by technology

The ion-selective electrode method has been used in clinical applications for the determination of ionized calcium. Although

Method used

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Embodiment

[0012] A method for detecting ionized calcium content in a blood sample, the specific steps comprising:

[0013] (1) Self-made calcium ion selective electrode: 33.5% of polytetrafluoroethylene, 63% of synthetic vegetable ester, 0.8% of sodium tetraphenylborate, 1.2% of laurate, and 1.2% of N-butylpyridine calcium hexafluorophosphate in terms of mass percentage , N, N, N', N'-tetracyclohexyl-3-oxaglutaramide 0.3% as a solute, mixed and stirred evenly with the solvent tetrahydrofuran, wherein the mass of the solvent is 6 times that of the solute, mixed and stirred at room temperature for 16 hours, Then volatilize for 60 hours to prepare a sensing film with a film thickness of 0.5mm, and put the sensing film on the glassy carbon electrode substrate to obtain a calcium ion selective electrode;

[0014] (2) Prepare calcium ion standard series solutions. At room temperature, establish a two-electrode system through a calcium ion selective electrode and a reference electrode, specifi...

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Abstract

The invention discloses a method for detecting the ionic calcium content of a blood sample, and belongs to the field of calcium ion detection. The method comprises the following steps: by adopting polytetrafluoroethylene, synthetic plant ester, sodium tetraphenylborate, laurate, N-butyl pyridine calcium hexafluorophosphate and N,N,N',N'-tetracyclohexyl-3-oxa-glutaramide as solutes, reacting with a solvent tetrahydrofuran to prepare a calcium ion sensing membrane, and filling the calcium ion sensing membrane on a glassy carbon electrode substrate to obtain a calcium ion selective electrode; and measuring the ionic calcium content of the blood sample by using an ion selective electrode method which is used for a self-made calcium ion selective electrode. The method disclosed by the invention can be used for rapidly measuring the calcium ion content of the blood sample, and is high in measurement accuracy, good in stability, high in specificity, simple in measurement step and low in test cost.

Description

technical field [0001] The invention relates to the field of calcium ion detection, in particular to a method for detecting ionized calcium content in blood samples. Background technique [0002] Blood calcium exists almost entirely in plasma and can be divided into two categories: diffuse calcium and non-diffusible calcium. Non-diffusible calcium refers to calcium bound to proteins (mainly albumin), accounting for about 40% of total plasma calcium. 60% is diffusible calcium. These two types of calcium exist in three forms: ① diffusible ionic calcium, which is a physiologically active part. ②Diffusible non-ionic calcium (accounting for about l5% of total serum calcium) is compound calcium combined with citric acid and bicarbonate. ③ Non-diffusible calcium is the calcium bound to plasma albumin, and its content is related to the concentration of plasma albumin. This part of calcium is not easy to penetrate the blood vessel wall and has no physiological activity. If the pla...

Claims

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Application Information

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IPC IPC(8): G01N27/333G01N33/49
Inventor 李宏奎巩晓东赵斌赵喜平贾继芳
Owner 李宏奎
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