Pretreatment method for rapid determination of beta-agonists in livestock and poultry meat through gas chromatography/mass spectrometry

A technology for rapid determination of livestock and poultry meat, applied in the field of pretreatment of beta-stimulants, can solve the problems of target loss, low accuracy, environmental pollution, etc., to achieve the effect of improving accuracy, fewer steps, and avoiding environmental pollution

Inactive Publication Date: 2015-08-19
QUZHOU QUALITY & TECH SUPERVISION & INSPECTION CENT
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Problems solved by technology

The traditional treatment method generally uses more than three kinds of solvents in the extraction process, which is easy to cause

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  • Pretreatment method for rapid determination of beta-agonists in livestock and poultry meat through gas chromatography/mass spectrometry

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[0023] A pretreatment method for rapid determination of β-stimulants in livestock and poultry meat by gas chromatography-mass method. Equipment and nitrogen blowing apparatus; reagents include: perchloric acid, methanol, ammonia water, toluene, N, O-bistrimethylsilane trifluoroacetamide (BSTFA) and standard products with a purity of ≥98%.

[0024] The gas chromatography-mass spectrometry instrument is an Agilent 7890-5975C phase chromatography-mass spectrometry instrument, and the structure of the chromatography and mass spectrometry is precise.

[0025] The standard products include albuterol, clenbuterol hydrochloride, terbutaline sulfate and ractopamine, which are four kinds of β-agonists.

[0026] The steps of a pretreatment method for rapid determination of beta-stimulants in livestock and poultry meat by temperament method are:

[0027] a. Preparation of standard solution: Accurately weigh 1 mg of the four standard products, dilute to 10 mL with methanol, prepare a 0.1 ...

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Abstract

The invention relates to a pretreatment method for rapid determination of beta-agonists in livestock and poultry meat through gas chromatography/mass spectrometry and discloses a simple, convenient, highly efficient and accurate pretreatment technology in determination of terbutaline, clenbuterol, salbutamol and ractopamine through gas chromatography/mass spectrometry. According to the method, a minced meat sample is ultrasonically extracted with 5% perchloric acid, then centrifuged at a speed of 10000 r/min for 15 min; then supernatant is enriched and purified with a WCX solid-phase extraction column; and detection is carried out after derivatization. The four agonists have good linearity in a range of 50 to 1000 [mu]g/L; correlation coefficient is more than 0.995; standard-added recovery rate is in a range of 84.32 to 103.15%; RSD is in a range of 1.35 to 4.78%; occurrence of false positive results is prevented; and lowest detection limit and the standard-added recovery rate meet current detection requirements at home and abroad.

Description

technical field [0001] The invention relates to a pretreatment method for rapid determination of beta-stimulant in livestock and poultry meat by temperament method. Background technique [0002] Terbutaline, clenbuterol, albuterol and ractopamine belong to β-adrenergic receptor stimulants, all of which can promote the transfer of nutrients from fat tissue to muscle tissue in animals. However, after animals eat feed containing terbutaline, clenbuterol, albuterol and ractopamine, the drug will remain in the muscles and tissues to varying degrees, and excessive intake of this drug by the human body will cause poisoning and other toxic side effects , In addition, it can also cause metabolic disorders, and acidosis or ketone poisoning can occur in diabetics. Therefore, many countries have strict regulations on the maximum residues of such drugs. Since my country has increased the supervision of the use of clenbuterol in recent years, some criminals have turned to illegal use of ...

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Application Information

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IPC IPC(8): G01N30/06
Inventor 郑睿行方建军张旭鲍利锋梅凯王键
Owner QUZHOU QUALITY & TECH SUPERVISION & INSPECTION CENT
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