Preparation method and application of photoelectrochemical sensor based on Au@TiO2/Bi2S3 modified electrode

A technology for modifying electrodes and photoelectrochemistry, applied in the direction of material electrochemical variables, etc., can solve the problem of low photoelectric conversion efficiency in the visible light region, and achieve the effects of excellent photoelectric conversion characteristics, increased sensitivity, and highly sensitive detection.

Inactive Publication Date: 2015-08-26
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the wide band gap (3.2 eV) of TiO2, it can only be effectively excited in the ultraviolet region, so the photoelectric conversion efficiency in the visible region is extremely low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 A based on AuTiO 2 / Bi 2 S 3 Preparation method of photoelectrochemical sensor for breast cancer marker CA15-3 with modified electrode

[0027] (1) AuTiO 2 / Bi 2 S 3 Preparation of the modified electrode: Cut the ITO conductive glass to a size of 2 cm × 0.5 cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen. 6 μL, 3 mg / mL AuTiO 2 The solution was added dropwise to the surface of the electrode, dried naturally at room temperature, and 6 μL, 3mg / mL Bi 2 S 3 The solution was added dropwise to the electrode surface and allowed to dry at room temperature.

[0028] (2) Add 6 μL, 8 μg / mL CA15-3 capture antibody Ab dropwise 1 On the surface of the electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0029] (3) Drop 3 μL of 0.5% BS solution A on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, and dry it in a refr...

Embodiment 2

[0031] Example 2 A based on AuTiO 2 / Bi 2 S 3 Preparation method of photoelectrochemical sensor for breast cancer marker CA15-3 with modified electrode

[0032] (1) AuTiO 2 / Bi 2 S 3 Preparation of the modified electrode: Cut the ITO conductive glass to a size of 2 cm × 0.5 cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen. 6 μL, 3 mg / mL AuTiO 2 The solution was added dropwise to the surface of the electrode, and dried naturally at room temperature, and 6 μL, 4mg / mL Bi 2 S 3 The solution was added dropwise to the electrode surface and allowed to dry at room temperature.

[0033] (2) Add 6 μL, 10 μg / mL CA15-3 capture antibody Ab dropwise 1 On the surface of the electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0034] (3) Add 3 μL of BS solution A with a mass fraction of 1.0% dropwise on the surface of the electrode to seal the non-specific active sites on the surface o...

Embodiment 3

[0036] Example 3 A based on AuTiO 2 / Bi 2 S 3 Preparation method of photoelectrochemical sensor for breast cancer marker CA15-3 with modified electrode

[0037] (1) AuTiO 2 / Bi 2 S 3 Preparation of the modified electrode: Cut the ITO conductive glass to a size of 2 cm × 0.5 cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen. 6 μL, 3 mg / mL AuTiO 2 The solution was added dropwise to the surface of the electrode, dried naturally at room temperature, and 6 μL, 5mg / mL Bi 2 S 3 The solution was added dropwise to the electrode surface and allowed to dry at room temperature.

[0038] (2) Add 6 μL, 12 μg / mL CA15-3 capture antibody Ab dropwise 1 On the surface of the electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0039] (3) Add 3 μL of BS solution A with a mass fraction of 2.0% on the surface of the electrode to seal the non-specific active sites on the surface of the electro...

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Abstract

The invention relates to a preparation method and an application of a photoelectrochemical sensor which is used for detection of a breast cancer marker CA15-3 and based on an Au@TiO2/Bi2S3 modified electrode, and belongs to the technical field of novel functional materials and biosensing detection. Specifically, an Au@TiO2 core-shell nanomaterial with photoelectrocatalysis activity and a Bi2S3 nanorod are compounded on an electrode, the marker-free photoelectrochemical sensor is prepared, and the breast cancer marker CA15-3 is rapidly detected in an ultra-sensitive manner. The method has a great significance in early diagnosis and judgment after healing of breast cancer.

Description

technical field [0001] The present invention relates to a kind of based on AuTiO 2 / Bi 2 S 3 Preparation method and application of a photoelectrochemical sensor for breast cancer marker CA15-3 with modified electrodes. Specifically, AuTiO with photocatalytic activity will be placed on the electrode 2 Core-shell nanomaterials and Bi 2 S 3 The nanorods are combined to prepare a label-free photoelectrochemical sensor to realize the rapid and ultra-sensitive detection of the breast cancer marker CA15-3. The invention belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Photoelectrochemical detection is an emerging detection method in bioanalysis. In the photoelectrochemical detection process, light is used as an excitation signal, and an electrical signal is used as a detection signal. Due to the complete separation of excitation and detection signals resulting in lower background signals, photoelectrochemistr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 柳懿芯魏琴马洪敏王晓东吴丹胡丽华范大伟颜兆庆王超刘振
Owner UNIV OF JINAN
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