Method for preparing high-purity 4-(2-hydroxyerhyl) piperazine-1-erhaesulfonic acid through nanofiltration

A technology of hydroxyethylpiperazineethanesulfonic acid and hydroxyethylpiperazine, which is applied in the field of organic matter preparation, can solve the problems of high salt content in wastewater, small treatment capacity, environmental pollution, etc., and achieve simple operation process and environmental protection in the production process , The effect of low purification cost

Inactive Publication Date: 2015-09-02
XZL BIO TECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The ion exchange resin method has the disadvantages of short life of ion exchange resin, frequent replacement, small processing capacity, and a large a

Method used

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  • Method for preparing high-purity 4-(2-hydroxyerhyl) piperazine-1-erhaesulfonic acid through nanofiltration
  • Method for preparing high-purity 4-(2-hydroxyerhyl) piperazine-1-erhaesulfonic acid through nanofiltration
  • Method for preparing high-purity 4-(2-hydroxyerhyl) piperazine-1-erhaesulfonic acid through nanofiltration

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Effect test

Embodiment 1

[0030] Add 0.5mol N-hydroxyethylpiperazine, 0.525mol sodium 2-isethionate and 500mL deionized water into a 1000mL four-necked flask with a reflux tube, and carry out condensation reaction under full stirring; React for 1.0 hour, then gradually increase the temperature and boil to reflux, and continue to react for 2.0 hours; then lower the temperature to obtain a reaction mother liquor containing sodium 4-hydroxyethylpiperazineethanesulfonate. Through high-performance liquid chromatography analysis, the calculated yield of sodium 4-hydroxyethylpiperazineethanesulfonate was 88.4%.

Embodiment 2

[0032] Add 0.5mol of N-hydroxyethylpiperazine, 0.575mol of sodium 2-isethionate and 500mL of methanol into a 1000mL four-neck flask with a reflux tube, and carry out condensation reaction under full stirring; first react at 50°C for 2.0 Hours, then gradually increase the temperature and boil to reflux, and continue to react for 3.0 hours; then cool down to obtain a reaction mother liquor containing 4-hydroxyethylpiperazine sodium ethanesulfonate. Through high-performance liquid chromatography analysis, the calculated yield of sodium 4-hydroxyethylpiperazineethanesulfonate was 93.6%.

Embodiment 3

[0034] Add 0.5mol N-hydroxyethylpiperazine, 0.5mol 2-isethionic acid and 500mL acetone into a 1000mL four-neck flask with a reflux tube, then slowly add aqueous sodium hydroxide solution (mass concentration of 30%, containing 1.1mol sodium hydroxide); then react at 40°C for 2.0 hours, then gradually heat up and boil to reflux, and continue to react for 1.5 hours; then cool down to obtain the reaction mother liquor containing 4-hydroxyethylpiperazine ethanesulfonate . Through high-performance liquid chromatography analysis, the calculated yield of sodium 4-hydroxyethylpiperazineethanesulfonate was 89.9%.

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Abstract

The invention belongs to the technical field of organic matter preparation, and particularly relates to a method for preparing a high-purity 4-(2-hydroxyerhyl) piperazine-1-erhaesulfonic acid through nanofiltration. The method comprises the following steps: firstly, carrying out a condensation reaction on a 2-isethionic acid or 2-isethionate and N-hydroxyethyl piperazine so as to obtain 4-hydroxyethyl piperazine esilate; by using an acidifier, converting the 4-hydroxyethyl piperazine esilate into a 4-(2-hydroxyerhyl) piperazine-1-erhaesulfonic acid and salts of corresponding acidifiers, so that acidified mother liquor is obtained; diluting the acidified mother liquor into a solution (1-10 wt %) by using deionized water, removing the salts of corresponding acidifiers through nanofiltration, and carrying out evaporation and concentration on the obtained object, so that a 4-(2-hydroxyerhyl) piperazine-1-erhaesulfonic acid crude product is obtained; and finally, washing and drying the 4-(2-hydroxyerhyl) piperazine-1-erhaesulfonic acid crude product, so that a high-purity 4-(2-hydroxyerhyl) piperazine-1-erhaesulfonic acid is obtained. The 4-(2-hydroxyerhyl) piperazine-1-erhaesulfonic acid prepared according to the invention is not only high in purity and simple in operation process, but also environmental-friendly in production process, low in purification cost, high in yield, and suitable for industrial mass production.

Description

technical field [0001] The invention belongs to the technical field of organic matter preparation, in particular to a method for preparing high-purity 4-hydroxyethylpiperazineethanesulfonic acid by nanofiltration. Background technique [0002] 4-Hydroxyethylpiperazineethanesulfonic acid (HEPES) is an important hydrogen ion buffer, which has good buffering capacity in the range of pH 6.8 to 8.2, and can control a constant pH for a long time. The use concentration is 10-50mmol / L, and the general culture medium containing 20mmol / L HEPES can have good buffering capacity and has no toxic effect on cells. [0003] Other applications of HEPES are also extensive. Patent CN201310477071.6 discloses a method for preparing porous zinc oxide microspheres using HEPES as a template. The method is simple to operate, and the prepared microspheres have the advantages of uniform size and multi-level pore structure, and can be used to produce catalysts and gas-sensing components. Patent CN20...

Claims

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Application Information

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IPC IPC(8): C07D295/088
CPCC07D295/088
Inventor 崔洪友朱丽伟王建刚刘然升杨涌王杨
Owner XZL BIO TECHNOLOGY CO LTD
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