Application of Lei Gonglian Extract in Preparation of Anti-myocardial Ischemia and Hypoxia Products

An anti-myocardial ischemia, extract technology, applied in cardiovascular system diseases, medical preparations containing active ingredients, drug combinations, etc., to achieve the effects of easy availability of raw materials, obvious activity, and improvement of normal pressure hypoxia tolerance

Active Publication Date: 2019-05-28
贵州中医药大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant research report on the use of Lei Gonglian in anti-myocardial ischemia and hypoxia products

Method used

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  • Application of Lei Gonglian Extract in Preparation of Anti-myocardial Ischemia and Hypoxia Products
  • Application of Lei Gonglian Extract in Preparation of Anti-myocardial Ischemia and Hypoxia Products
  • Application of Lei Gonglian Extract in Preparation of Anti-myocardial Ischemia and Hypoxia Products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: Preparation of Lei Gonglian Extract

[0050] Soak Lei Gonglian medicinal material in 95%, 75%, and 50% ethanol respectively for 4 times, each time for 15 hours, until the color becomes lighter, combine the soaking liquid, recover the ethanol, and obtain the ethanol extract; place the ethanol extract at 70°C Volatilize in a water bath until there is no alcohol smell, first extract 4 times with 1.5 times the volume of petroleum ether, discard the petroleum ether extract and aqueous solution, then extract 4 times with 1.5 times the volume of ethyl acetate, discard the ethyl acetate extract and aqueous solution Finally, extract 5 times with 2 times the volume of n-butanol, take the n-butanol extract and recover it under reduced pressure until it has no alcohol smell, and obtain the n-butanol extract, dissolve it in water, put it on a macroporous resin column, and wash it with distilled water first. After the eluate is colorless, use 12 times column volume of 5...

Embodiment 2

[0051] Embodiment 2: the preparation of Lei Gonglian extract

[0052] Soak Lei Gonglian medicinal material in 95%, 75%, and 50% ethanol respectively for 3 times, each time for 24 hours, until the color becomes lighter, combine the soaking liquid, recover the ethanol, and obtain the ethanol extract; place the ethanol extract at 70°C Volatilize in a water bath until there is no alcohol smell, first extract 5 times with 0.5 times the volume of petroleum ether, discard the petroleum ether extract and aqueous solution, then extract 3 times with 2 times the volume of ethyl acetate, discard the ethyl acetate extract and aqueous solution Finally, extract 6 times with 0.5 times the volume of n-butanol, take the n-butanol extract and recover it under reduced pressure until it has no alcohol smell, and obtain the n-butanol extract. After dissolving in water, put it on a macroporous resin column and wash it with distilled water first. After the eluate is colorless, elute with 8 times colu...

Embodiment 3

[0053] Embodiment 3: the preparation of Lei Gonglian extract

[0054] Soak Lei Gonglian medicinal material in 95%, 75%, and 50% ethanol respectively for 5 times, each time for 6 hours, until the color becomes lighter, combine the soaking liquid, recover the ethanol, and obtain the ethanol extract; place the ethanol extract at 70°C Volatilize in a water bath until there is no alcohol smell, first extract 3 times with 2 times the volume of petroleum ether, then extract 5 times with 0.5 times the volume of ethyl acetate, and finally extract 3 times with 3 times the volume of n-butanol, take n-butanol The extract is recovered under reduced pressure until it has no alcohol smell, and the n-butanol extract is obtained. After dissolving in water, put it on a macroporous resin column, wash it with distilled water until the eluate is colorless, and then use 50% of 15 times the column volume. Ethanol was eluted at a rate of 2 times the column volume / h, the ethanol eluate was collected, ...

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Abstract

The invention discloses an application of Lei Gonglian extract in preparing anti-myocardial ischemia and hypoxia products. The present invention has carried out in-depth research on the activity of the extract of Lei Gonglian, through the impact on the T wave of the electrocardiogram of acute myocardial ischemia in mice induced by ISO, the impact on the ability of mice to withstand hypoxia under normal pressure, and the effect on acute myocardial ischemia in mice induced by ISO. The influence test of blood normal pressure hypoxia tolerance and the impact test on mouse decapitation ischemia hypoxia wheezing time, it can be seen that the extract of Lei Gonglian can effectively inhibit the elevation of myocardial ST-T, and improve the normal pressure hypoxia tolerance of normal mice. Oxygen capacity, improve the ability of mice to withstand acute myocardial ischemia and normal pressure hypoxia caused by ISO, and prolong the time of decapitated mice ischemic and hypoxic wheezing, indicating that the extract of Lei Gonglian has a significant effect on myocardial ischemia and hypoxia. Therefore, the extract of Lei Gonglian has obvious activity, stable performance, safety and reliability, and can be used to prepare anti-myocardial ischemia and hypoxia products. In addition, the raw material is easy to obtain, the extraction process is simple, and the industrial production is easy.

Description

technical field [0001] The invention relates to the application of Lei Gonglian extract in the preparation of anti-myocardial ischemia and hypoxia products, and belongs to the technical field of application of active ingredients of medicinal materials. Background technique [0002] Myocardial ischemic disease is a focus of cardiovascular diseases, and myocardial ischemia and hypoxia are also the common pathological basis of myocardial infarction and ischemic heart disease. Insufficient myocardial blood supply and oxygen supply lead to myocardial metabolic disorder, insufficient energy supply, decreased myocardial contractile function, and reduced blood output, which in turn affects the function of the entire body and even causes myocardial cell death. Therefore, clinical research and experimental research on cardiovascular diseases, especially myocardial ischemia and hypoxia, have generally received attention. [0003] Myocardial ischemia and hypoxia belong to the category ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/888A61P9/10A61P9/00A23L33/105
Inventor 吴红梅王祥培李欣灿王远敏钱海兵
Owner 贵州中医药大学
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